Boiling Springs Lake RNA-DNA hybrid virus

Taxonomy: Viruses; environmental samples

Average proteome isoelectric point is 8.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0CKB4|I0CKB4_9VIRU ORF-4 OS=Boiling Springs Lake RNA-DNA hybrid virus OX=1379788 PE=4 SV=1
MM1 pKa = 7.78SKK3 pKa = 10.0RR4 pKa = 11.84QPFSYY9 pKa = 10.52RR10 pKa = 11.84LQSLNYY16 pKa = 8.39TFARR20 pKa = 11.84NPPRR24 pKa = 11.84GVRR27 pKa = 11.84ISKK30 pKa = 10.4DD31 pKa = 3.54LLHH34 pKa = 6.91AMYY37 pKa = 8.74VTKK40 pKa = 10.87VNGEE44 pKa = 3.85QYY46 pKa = 10.83AWNMNDD52 pKa = 2.94NDD54 pKa = 3.54AWPNGICPDD63 pKa = 3.12SFGNVKK69 pKa = 9.91IKK71 pKa = 10.59RR72 pKa = 11.84ALKK75 pKa = 10.32DD76 pKa = 3.29LSYY79 pKa = 11.49DD80 pKa = 3.67NSPVNPDD87 pKa = 3.25TPFGRR92 pKa = 11.84LQTRR96 pKa = 11.84GLCALFSVLFKK107 pKa = 11.26RR108 pKa = 11.84MVEE111 pKa = 3.89NLGPDD116 pKa = 3.33EE117 pKa = 4.67ALSEE121 pKa = 4.11ILNPRR126 pKa = 11.84VNWLEE131 pKa = 4.14LINFFIYY138 pKa = 10.06EE139 pKa = 3.84LHH141 pKa = 6.5

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0CKB4|I0CKB4_9VIRU ORF-4 OS=Boiling Springs Lake RNA-DNA hybrid virus OX=1379788 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 4.08MTDD5 pKa = 3.37EE6 pKa = 4.92DD7 pKa = 3.52IEE9 pKa = 5.12RR10 pKa = 11.84ILRR13 pKa = 11.84HH14 pKa = 5.2TLPPVSHH21 pKa = 5.99VTNEE25 pKa = 4.76DD26 pKa = 3.3ILFQPWWDD34 pKa = 3.48MGKK37 pKa = 9.61GHH39 pKa = 8.32AIPDD43 pKa = 3.57YY44 pKa = 9.61MAKK47 pKa = 10.54DD48 pKa = 3.58FNNPFEE54 pKa = 4.18FYY56 pKa = 10.65KK57 pKa = 11.15YY58 pKa = 10.42FLSKK62 pKa = 10.32NNKK65 pKa = 8.57RR66 pKa = 11.84FLYY69 pKa = 9.17YY70 pKa = 10.77LSRR73 pKa = 11.84TFIKK77 pKa = 10.47KK78 pKa = 10.57FSFNKK83 pKa = 9.1AKK85 pKa = 10.52RR86 pKa = 11.84YY87 pKa = 7.26GRR89 pKa = 11.84KK90 pKa = 8.66WSEE93 pKa = 3.67EE94 pKa = 3.63GWRR97 pKa = 11.84KK98 pKa = 10.16LIDD101 pKa = 3.35YY102 pKa = 10.38CDD104 pKa = 3.88YY105 pKa = 11.05IKK107 pKa = 11.25NSLSLKK113 pKa = 9.97KK114 pKa = 11.04YY115 pKa = 9.71NITVKK120 pKa = 10.07EE121 pKa = 3.95RR122 pKa = 11.84NEE124 pKa = 3.67IFEE127 pKa = 6.07KK128 pKa = 10.58IMEE131 pKa = 4.92DD132 pKa = 2.75NGYY135 pKa = 9.81FLPP138 pKa = 5.0

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

821

138

542

273.7

30.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.795 ± 2.336

0.853 ± 0.169

4.994 ± 0.847

3.776 ± 1.213

5.603 ± 0.794

7.674 ± 1.831

2.314 ± 0.303

5.116 ± 0.662

5.968 ± 1.779

8.283 ± 0.907

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.579

6.699 ± 1.098

4.507 ± 0.8

3.654 ± 1.09

4.629 ± 0.985

7.065 ± 0.764

7.065 ± 2.041

5.359 ± 1.026

1.583 ± 0.522

4.872 ± 0.742
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski