Circovirus-like genome DCCV-4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHI9|A0A190WHI9_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-4 OX=1788444 PE=4 SV=1
MM1 pKa = 6.74KK2 pKa = 9.5TAMTCSEE9 pKa = 4.46TLSLDD14 pKa = 2.93KK15 pKa = 11.33SEE17 pKa = 4.93NVFLNPLISPIWPDD31 pKa = 3.17YY32 pKa = 7.94SQRR35 pKa = 11.84MPWTYY40 pKa = 10.0PLTVIDD46 pKa = 4.46PQTLQSQEE54 pKa = 4.04LFQTLSSSEE63 pKa = 4.15SEE65 pKa = 4.22EE66 pKa = 4.0EE67 pKa = 4.13EE68 pKa = 3.9EE69 pKa = 4.89VDD71 pKa = 5.16FEE73 pKa = 4.87TEE75 pKa = 3.69FAEE78 pKa = 5.19IPTPTQPHH86 pKa = 5.7RR87 pKa = 11.84HH88 pKa = 5.39QYY90 pKa = 9.21HH91 pKa = 6.85PLYY94 pKa = 10.36QKK96 pKa = 10.94SLTRR100 pKa = 11.84EE101 pKa = 3.79YY102 pKa = 10.63FRR104 pKa = 11.84PLWQQLEE111 pKa = 4.11PDD113 pKa = 4.42AFDD116 pKa = 4.25PLYY119 pKa = 11.14DD120 pKa = 4.12PFLDD124 pKa = 3.73NN125 pKa = 5.22

Molecular weight: 14.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHI9|A0A190WHI9_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-4 OX=1788444 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84PNYY5 pKa = 9.28KK6 pKa = 10.0RR7 pKa = 11.84KK8 pKa = 10.11LYY10 pKa = 10.7EE11 pKa = 3.73DD12 pKa = 3.75TFNDD16 pKa = 2.84RR17 pKa = 11.84MKK19 pKa = 10.65RR20 pKa = 11.84IRR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 3.77EE25 pKa = 3.94MQDD28 pKa = 2.79AVYY31 pKa = 10.35RR32 pKa = 11.84ALPRR36 pKa = 11.84GGVPAVNRR44 pKa = 11.84PAQRR48 pKa = 11.84AGYY51 pKa = 9.98GSTARR56 pKa = 11.84ARR58 pKa = 11.84GAAVTGEE65 pKa = 4.13MKK67 pKa = 10.96YY68 pKa = 10.65FDD70 pKa = 4.66CEE72 pKa = 4.03RR73 pKa = 11.84DD74 pKa = 3.2SGAISAVTTTWVAGCMVDD92 pKa = 3.19PTRR95 pKa = 11.84TINLGDD101 pKa = 3.78ASVATPLCLFAPKK114 pKa = 10.17VSAALNGRR122 pKa = 11.84IGRR125 pKa = 11.84KK126 pKa = 7.54AHH128 pKa = 5.38VYY130 pKa = 9.21KK131 pKa = 10.92VRR133 pKa = 11.84IAGTIKK139 pKa = 10.34IPPQTAQGTADD150 pKa = 3.64ASPTIRR156 pKa = 11.84LALVLDD162 pKa = 4.07KK163 pKa = 11.09QSNAAQMTAATLFNDD178 pKa = 3.5AAGAEE183 pKa = 4.28TTITSFQNPNNFGRR197 pKa = 11.84FRR199 pKa = 11.84VLKK202 pKa = 9.1EE203 pKa = 3.57KK204 pKa = 10.79YY205 pKa = 8.88YY206 pKa = 10.73QFSNVNLAGDD216 pKa = 3.84PTAANLVQCGCTINFKK232 pKa = 10.26MSVSFKK238 pKa = 10.99QPVQVNFNATNGGTVADD255 pKa = 4.78IIDD258 pKa = 3.85NSLHH262 pKa = 5.72IVCGATSVAMAPVIAYY278 pKa = 7.81YY279 pKa = 10.88SRR281 pKa = 11.84VCYY284 pKa = 10.47KK285 pKa = 10.41EE286 pKa = 3.65

Molecular weight: 31.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

812

125

401

270.7

30.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.867 ± 2.438

2.094 ± 0.378

6.158 ± 0.699

5.296 ± 1.813

4.187 ± 0.411

6.404 ± 1.88

1.847 ± 0.489

4.557 ± 0.395

3.941 ± 0.533

7.759 ± 1.394

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.279

3.818 ± 1.061

5.419 ± 1.698

5.049 ± 0.9

7.02 ± 1.123

7.02 ± 0.944

7.882 ± 0.306

5.911 ± 1.204

1.232 ± 0.446

3.325 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski