Hubei tombus-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGK3|A0A1L3KGK3_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 23 OX=1923270 PE=4 SV=1
MM1 pKa = 7.62GICLGSLEE9 pKa = 4.17PSKK12 pKa = 10.79LIKK15 pKa = 10.63AKK17 pKa = 10.16ILNIKK22 pKa = 8.72TDD24 pKa = 3.58PCQHH28 pKa = 5.88KK29 pKa = 9.24TYY31 pKa = 9.78IYY33 pKa = 10.06PLINPPPGIDD43 pKa = 3.11HH44 pKa = 5.59QTQVVYY50 pKa = 9.67QPCQLNEE57 pKa = 3.84LHH59 pKa = 6.65SLNKK63 pKa = 9.65RR64 pKa = 11.84HH65 pKa = 6.4LVFHH69 pKa = 6.97KK70 pKa = 9.87WPKK73 pKa = 8.21EE74 pKa = 3.74QLVNEE79 pKa = 5.63AIQHH83 pKa = 5.33TKK85 pKa = 10.37QLLKK89 pKa = 10.85DD90 pKa = 3.86GEE92 pKa = 4.56NVQPITPMEE101 pKa = 3.88YY102 pKa = 10.74AMTRR106 pKa = 11.84KK107 pKa = 8.81GAKK110 pKa = 8.78RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84YY114 pKa = 8.3QQAAEE119 pKa = 3.92EE120 pKa = 3.85LDD122 pKa = 3.96YY123 pKa = 11.92YY124 pKa = 10.99GMKK127 pKa = 10.36PSATNVTAFIKK138 pKa = 10.05IEE140 pKa = 3.66KK141 pKa = 8.4WDD143 pKa = 3.89RR144 pKa = 11.84EE145 pKa = 4.14VAMVKK150 pKa = 9.67SPRR153 pKa = 11.84MIQYY157 pKa = 8.78RR158 pKa = 11.84HH159 pKa = 5.45PTYY162 pKa = 10.87VSTIASRR169 pKa = 11.84LYY171 pKa = 10.14PFEE174 pKa = 4.27EE175 pKa = 4.41KK176 pKa = 10.41LWEE179 pKa = 4.15TEE181 pKa = 4.03RR182 pKa = 11.84NGCRR186 pKa = 11.84VFAKK190 pKa = 10.65GLDD193 pKa = 3.8SYY195 pKa = 10.07QTAAQLRR202 pKa = 11.84MKK204 pKa = 9.96WEE206 pKa = 4.01RR207 pKa = 11.84CDD209 pKa = 3.57RR210 pKa = 11.84PIAIMKK216 pKa = 9.83DD217 pKa = 3.45FSKK220 pKa = 10.71FDD222 pKa = 3.56SCITLPWIKK231 pKa = 10.47GEE233 pKa = 4.05KK234 pKa = 9.54EE235 pKa = 3.62IYY237 pKa = 10.05EE238 pKa = 4.17LTGTMDD244 pKa = 3.09EE245 pKa = 4.78MDD247 pKa = 3.57YY248 pKa = 11.21QFNNTCSTVNGVSYY262 pKa = 10.49KK263 pKa = 10.71CEE265 pKa = 3.66ARR267 pKa = 11.84KK268 pKa = 9.14MSGEE272 pKa = 4.23YY273 pKa = 8.66NTSCGDD279 pKa = 4.38SIVNWSATDD288 pKa = 3.48KK289 pKa = 10.34AFHH292 pKa = 6.95MITGHH297 pKa = 4.89YY298 pKa = 9.57RR299 pKa = 11.84YY300 pKa = 9.99YY301 pKa = 10.55PNINGDD307 pKa = 4.19DD308 pKa = 3.61DD309 pKa = 5.24VMIVDD314 pKa = 4.12EE315 pKa = 5.06SEE317 pKa = 3.7IGDD320 pKa = 3.59IEE322 pKa = 4.32EE323 pKa = 4.56FLPKK327 pKa = 10.37LIEE330 pKa = 4.14NLEE333 pKa = 4.06LLGFKK338 pKa = 9.8TDD340 pKa = 3.09ASYY343 pKa = 10.61TRR345 pKa = 11.84TFEE348 pKa = 4.68EE349 pKa = 4.91IEE351 pKa = 4.33FCQSQPVEE359 pKa = 4.13VEE361 pKa = 4.11SNIWRR366 pKa = 11.84MIRR369 pKa = 11.84KK370 pKa = 7.14PARR373 pKa = 11.84AISRR377 pKa = 11.84DD378 pKa = 3.65CYY380 pKa = 10.43SVRR383 pKa = 11.84KK384 pKa = 9.95YY385 pKa = 11.06GGTAWYY391 pKa = 10.04RR392 pKa = 11.84LAASLGYY399 pKa = 10.03CEE401 pKa = 4.8YY402 pKa = 11.03ALNDD406 pKa = 4.17GVPVLQSWAKK416 pKa = 9.01YY417 pKa = 8.06LQRR420 pKa = 11.84SSKK423 pKa = 9.73GKK425 pKa = 9.98KK426 pKa = 9.3VLNQEE431 pKa = 2.93IEE433 pKa = 4.11YY434 pKa = 9.97RR435 pKa = 11.84VKK437 pKa = 11.02VEE439 pKa = 4.19IKK441 pKa = 10.67NKK443 pKa = 8.05TKK445 pKa = 10.51YY446 pKa = 8.62RR447 pKa = 11.84TTIDD451 pKa = 3.27PVARR455 pKa = 11.84EE456 pKa = 3.96SFAIAFGIEE465 pKa = 3.91PTQQIAIEE473 pKa = 4.18SWLDD477 pKa = 3.52NQEE480 pKa = 4.24SPEE483 pKa = 4.07LLPYY487 pKa = 10.28SEE489 pKa = 5.71GYY491 pKa = 8.63WGG493 pKa = 3.75

Molecular weight: 57.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGK3|A0A1L3KGK3_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 23 OX=1923270 PE=4 SV=1
MM1 pKa = 7.53NDD3 pKa = 3.16IIKK6 pKa = 10.7SKK8 pKa = 10.61EE9 pKa = 3.96EE10 pKa = 3.79PVQDD14 pKa = 3.69ISGTTPSTADD24 pKa = 3.19EE25 pKa = 4.33SRR27 pKa = 11.84ASFGKK32 pKa = 10.66APGEE36 pKa = 4.24GFQSKK41 pKa = 9.88PIQTNQSMKK50 pKa = 10.6KK51 pKa = 8.25NSKK54 pKa = 9.69VNSSQRR60 pKa = 11.84RR61 pKa = 11.84AAEE64 pKa = 4.02RR65 pKa = 11.84KK66 pKa = 9.44NEE68 pKa = 4.05RR69 pKa = 11.84DD70 pKa = 3.36DD71 pKa = 3.59KK72 pKa = 11.87ANVSNHH78 pKa = 4.5AVKK81 pKa = 10.81NGLSGGMRR89 pKa = 11.84FKK91 pKa = 10.96FNKK94 pKa = 9.44KK95 pKa = 9.94SKK97 pKa = 9.04LTYY100 pKa = 9.98DD101 pKa = 3.24QQKK104 pKa = 11.34ANFEE108 pKa = 4.26AMLNPVQKK116 pKa = 10.92ANNNPQDD123 pKa = 4.07FPNKK127 pKa = 7.46WRR129 pKa = 11.84QVAARR134 pKa = 11.84NKK136 pKa = 10.41RR137 pKa = 11.84KK138 pKa = 9.45DD139 pKa = 3.57AKK141 pKa = 10.73SATQRR146 pKa = 11.84QCAFTGRR153 pKa = 11.84QYY155 pKa = 11.11IATNATQRR163 pKa = 11.84LIEE166 pKa = 4.41KK167 pKa = 9.45RR168 pKa = 11.84NQSIHH173 pKa = 6.05HH174 pKa = 6.33LRR176 pKa = 11.84MEE178 pKa = 4.16NQTIRR183 pKa = 11.84RR184 pKa = 11.84EE185 pKa = 4.11LQSLKK190 pKa = 10.85EE191 pKa = 4.44CINDD195 pKa = 3.36LTKK198 pKa = 10.97LLRR201 pKa = 11.84GGKK204 pKa = 7.55QQNLRR209 pKa = 11.84KK210 pKa = 8.47PTLNSTKK217 pKa = 10.38SGEE220 pKa = 4.26SSSKK224 pKa = 10.22KK225 pKa = 10.16SRR227 pKa = 11.84TSEE230 pKa = 3.85VGTMSEE236 pKa = 4.25MNSEE240 pKa = 4.28RR241 pKa = 11.84FTTRR245 pKa = 11.84QSLDD249 pKa = 3.32PLKK252 pKa = 10.09RR253 pKa = 11.84TQSLQCLSYY262 pKa = 11.06KK263 pKa = 10.53DD264 pKa = 4.8ALLKK268 pKa = 10.48HH269 pKa = 6.48LNVKK273 pKa = 9.56PKK275 pKa = 10.56SITRR279 pKa = 11.84TSSLKK284 pKa = 10.97LNTKK288 pKa = 7.28QEE290 pKa = 4.46RR291 pKa = 11.84KK292 pKa = 10.39HH293 pKa = 5.63MMNSTEE299 pKa = 3.9QSTSAMRR306 pKa = 11.84NISSGSEE313 pKa = 3.59RR314 pKa = 11.84NKK316 pKa = 8.18QTKK319 pKa = 9.38GCQKK323 pKa = 10.5AEE325 pKa = 4.25SRR327 pKa = 11.84GHH329 pKa = 4.75QAQNVSTKK337 pKa = 9.76RR338 pKa = 11.84SRR340 pKa = 11.84MTTRR344 pKa = 11.84HH345 pKa = 5.4STDD348 pKa = 2.66GTQVIKK354 pKa = 10.51MEE356 pKa = 4.71SKK358 pKa = 8.42GTAPEE363 pKa = 3.94THH365 pKa = 6.26LTDD368 pKa = 4.2SGMQTEE374 pKa = 4.44EE375 pKa = 3.93VDD377 pKa = 3.18NEE379 pKa = 4.18TRR381 pKa = 11.84DD382 pKa = 3.86TIVDD386 pKa = 3.7DD387 pKa = 4.29VRR389 pKa = 11.84LINQGLSEE397 pKa = 4.64EE398 pKa = 4.6DD399 pKa = 3.87SLPKK403 pKa = 10.49PMIATKK409 pKa = 10.11IANKK413 pKa = 10.11GKK415 pKa = 10.23EE416 pKa = 3.7SGNRR420 pKa = 11.84FKK422 pKa = 11.22RR423 pKa = 11.84NLLKK427 pKa = 10.4QRR429 pKa = 11.84EE430 pKa = 4.31PVLQLPLGEE439 pKa = 4.17IQEE442 pKa = 4.25EE443 pKa = 4.54LISYY447 pKa = 9.52LRR449 pKa = 11.84IKK451 pKa = 10.78AGFHH455 pKa = 6.94AMDD458 pKa = 3.02TMMIRR463 pKa = 11.84RR464 pKa = 11.84LFQHH468 pKa = 6.66AEE470 pKa = 3.61TWADD474 pKa = 3.77KK475 pKa = 10.63YY476 pKa = 11.09CKK478 pKa = 9.64EE479 pKa = 4.63LSWQEE484 pKa = 3.96KK485 pKa = 9.23EE486 pKa = 4.35RR487 pKa = 11.84LIADD491 pKa = 4.09GVVTAMVPSEE501 pKa = 3.88HH502 pKa = 6.46MMVLRR507 pKa = 11.84QKK509 pKa = 11.04LKK511 pKa = 11.08DD512 pKa = 3.34SDD514 pKa = 4.19VQSKK518 pKa = 7.57LHH520 pKa = 6.45KK521 pKa = 10.52ASDD524 pKa = 3.64MAKK527 pKa = 10.45GDD529 pKa = 3.47LGKK532 pKa = 10.81RR533 pKa = 11.84NFFGKK538 pKa = 10.29EE539 pKa = 3.21ISLPTASKK547 pKa = 10.97

Molecular weight: 62.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1040

493

547

520.0

59.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.05

1.635 ± 0.507

4.615 ± 0.032

7.596 ± 0.586

2.596 ± 0.155

5.0 ± 0.045

2.019 ± 0.123

5.577 ± 1.094

9.423 ± 1.088

7.019 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.365 ± 0.333

5.385 ± 0.714

4.038 ± 0.655

5.865 ± 0.761

6.538 ± 0.674

8.077 ± 1.264

6.538 ± 0.545

4.135 ± 0.465

1.346 ± 0.561

3.269 ± 1.658
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski