Apis mellifera associated microvirus 9

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTX7|A0A3S8UTX7_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 9 OX=2494796 PE=4 SV=1
MM1 pKa = 7.27HH2 pKa = 7.49ASNYY6 pKa = 9.99KK7 pKa = 8.94MPSNMTHH14 pKa = 5.99NFSRR18 pKa = 11.84VPQVHH23 pKa = 5.96NPRR26 pKa = 11.84STFVSPYY33 pKa = 8.78TYY35 pKa = 9.28KK36 pKa = 10.25TALNAGFLYY45 pKa = 10.06PFFMDD50 pKa = 3.71EE51 pKa = 4.4VLPGDD56 pKa = 3.99TFNDD60 pKa = 3.61DD61 pKa = 3.38FTVFARR67 pKa = 11.84LTTPIKK73 pKa = 10.43PIMDD77 pKa = 4.32NLYY80 pKa = 10.07LDD82 pKa = 3.56TFFFYY87 pKa = 10.39IPNRR91 pKa = 11.84LLDD94 pKa = 4.11DD95 pKa = 3.29TWEE98 pKa = 4.35KK99 pKa = 11.43LNGAQDD105 pKa = 3.75NPGDD109 pKa = 4.17SIDD112 pKa = 3.84YY113 pKa = 9.17VVPQIPAPAVTGFASEE129 pKa = 4.43SLFDD133 pKa = 3.83YY134 pKa = 10.35MGIRR138 pKa = 11.84PGVTNFSIDD147 pKa = 3.18AYY149 pKa = 10.98HH150 pKa = 6.88PLAYY154 pKa = 10.57NLLFNTNFRR163 pKa = 11.84DD164 pKa = 3.47EE165 pKa = 4.24NLQDD169 pKa = 3.11SVYY172 pKa = 10.06FSKK175 pKa = 11.24GPGPHH180 pKa = 6.98AATNFNLLRR189 pKa = 11.84RR190 pKa = 11.84GKK192 pKa = 10.18RR193 pKa = 11.84FDD195 pKa = 3.87YY196 pKa = 8.15FTSCLPFPQKK206 pKa = 10.69GPEE209 pKa = 3.76VTIPILGDD217 pKa = 3.34APLIGIGTKK226 pKa = 9.98TNVGFSPGVTGYY238 pKa = 10.75DD239 pKa = 3.14YY240 pKa = 11.43DD241 pKa = 3.91GAVTYY246 pKa = 8.07TQGRR250 pKa = 11.84ATDD253 pKa = 3.58AGGANNAIIVDD264 pKa = 3.87VDD266 pKa = 3.71PATGLPKK273 pKa = 9.73WAASMAEE280 pKa = 3.91VSGVSINQFRR290 pKa = 11.84TSYY293 pKa = 9.7QIQMLLEE300 pKa = 4.04NDD302 pKa = 3.13ARR304 pKa = 11.84GGTRR308 pKa = 11.84YY309 pKa = 9.96PEE311 pKa = 3.8ILRR314 pKa = 11.84AHH316 pKa = 6.7FNVISPDD323 pKa = 3.24FRR325 pKa = 11.84LQRR328 pKa = 11.84PEE330 pKa = 3.9YY331 pKa = 10.47LGGTSVPVTVTPVPQTSSTDD351 pKa = 3.27NTSPQGNLAAYY362 pKa = 7.08ATSLNRR368 pKa = 11.84CGYY371 pKa = 7.33TKK373 pKa = 10.66SFVEE377 pKa = 4.15HH378 pKa = 6.35GVILGLCSIRR388 pKa = 11.84ADD390 pKa = 3.38LTYY393 pKa = 10.61QYY395 pKa = 11.07GLRR398 pKa = 11.84RR399 pKa = 11.84MWSRR403 pKa = 11.84QTRR406 pKa = 11.84YY407 pKa = 10.68DD408 pKa = 3.82FYY410 pKa = 10.82WPAFAHH416 pKa = 5.97LGEE419 pKa = 4.15MAVLNKK425 pKa = 10.06EE426 pKa = 4.09IYY428 pKa = 9.82IQGTADD434 pKa = 3.5DD435 pKa = 4.53NLVFGYY441 pKa = 10.16QEE443 pKa = 3.16AWADD447 pKa = 3.88YY448 pKa = 9.56KK449 pKa = 10.59YY450 pKa = 10.56HH451 pKa = 7.31PSLITGKK458 pKa = 8.75MRR460 pKa = 11.84SQDD463 pKa = 3.81PQSLDD468 pKa = 2.74IWHH471 pKa = 7.5LSQEE475 pKa = 4.43FQSLPLLNDD484 pKa = 3.05EE485 pKa = 5.48FIVDD489 pKa = 3.7RR490 pKa = 11.84TDD492 pKa = 3.78DD493 pKa = 3.57VVNRR497 pKa = 11.84ISAVQDD503 pKa = 3.19EE504 pKa = 4.49PQFYY508 pKa = 10.17FDD510 pKa = 5.37CFGHH514 pKa = 6.93LKK516 pKa = 10.17RR517 pKa = 11.84ARR519 pKa = 11.84QMPVYY524 pKa = 8.72STPGLITRR532 pKa = 11.84LL533 pKa = 3.6

Molecular weight: 59.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTX7|A0A3S8UTX7_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 9 OX=2494796 PE=4 SV=1
MM1 pKa = 7.56GLLSSIGKK9 pKa = 9.73AVGSITGGDD18 pKa = 3.45LLGFGGTLATGLLSNAGASAAQNFSAAQTQSQMDD52 pKa = 3.67FQEE55 pKa = 4.45RR56 pKa = 11.84MSNTAHH62 pKa = 5.61QRR64 pKa = 11.84QVADD68 pKa = 4.09LRR70 pKa = 11.84AAGLNPLLSATYY82 pKa = 9.67GGSSTPSGASAVGIDD97 pKa = 4.63KK98 pKa = 8.96ITPALASARR107 pKa = 11.84EE108 pKa = 4.09SARR111 pKa = 11.84VNAEE115 pKa = 4.2LKK117 pKa = 10.89NMQATNSKK125 pKa = 9.7IDD127 pKa = 3.08SDD129 pKa = 3.92TRR131 pKa = 11.84LNNALTVSAKK141 pKa = 10.61ADD143 pKa = 3.83ALSKK147 pKa = 10.4AASARR152 pKa = 11.84ASNAAADD159 pKa = 4.06TTLAMLPGRR168 pKa = 11.84EE169 pKa = 4.03AQSKK173 pKa = 10.48NSAKK177 pKa = 9.67EE178 pKa = 3.94AKK180 pKa = 10.15SFWATHH186 pKa = 4.47IRR188 pKa = 11.84TRR190 pKa = 11.84TKK192 pKa = 10.56PFFEE196 pKa = 5.13SIGDD200 pKa = 3.5ATGAVGNLFSGSKK213 pKa = 9.52QDD215 pKa = 3.19SGSMSRR221 pKa = 11.84FLSGGLL227 pKa = 3.47

Molecular weight: 23.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1352

87

533

270.4

30.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.876 ± 1.608

1.109 ± 0.634

6.731 ± 0.425

3.846 ± 0.66

5.251 ± 0.597

7.544 ± 0.63

2.441 ± 0.414

3.846 ± 0.47

4.29 ± 0.834

7.766 ± 0.489

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.225

4.808 ± 0.501

5.991 ± 1.145

3.624 ± 0.562

6.361 ± 0.832

7.988 ± 1.142

6.509 ± 0.593

5.547 ± 0.566

0.962 ± 0.232

4.512 ± 0.945
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski