Proteobacteria bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; unclassified Proteobacteria

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q6B808|A0A4Q6B808_9PROT Methyltransferase domain-containing protein OS=Proteobacteria bacterium OX=1977087 GN=EOP07_07600 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.65YY3 pKa = 10.26FIPKK7 pKa = 9.46IFVIILASAPSFGYY21 pKa = 10.01SQAAQINGNTSSEE34 pKa = 4.21PAVRR38 pKa = 11.84AADD41 pKa = 3.69EE42 pKa = 4.36NVVPNYY48 pKa = 10.78NFAFPQNTTVNEE60 pKa = 4.11RR61 pKa = 11.84WSSEE65 pKa = 3.87GQVEE69 pKa = 4.7VPTEE73 pKa = 4.09GQVQFPVGGQVTFEE87 pKa = 4.41PEE89 pKa = 3.91GQVEE93 pKa = 4.35DD94 pKa = 5.03FEE96 pKa = 5.05TGNIPPYY103 pKa = 10.55VYY105 pKa = 10.58AVPNEE110 pKa = 4.29MEE112 pKa = 4.78AQTQMQNQVQTYY124 pKa = 8.58VDD126 pKa = 3.87PNAGQTEE133 pKa = 4.21EE134 pKa = 4.74QYY136 pKa = 11.3VEE138 pKa = 4.08PTVDD142 pKa = 3.75ANMEE146 pKa = 4.19VQTDD150 pKa = 3.9PNAGMTVDD158 pKa = 3.49RR159 pKa = 5.33

Molecular weight: 17.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2AXH3|A0A4V2AXH3_9PROT PAS domain S-box protein (Fragment) OS=Proteobacteria bacterium OX=1977087 GN=EOP07_14815 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.16RR13 pKa = 11.84KK14 pKa = 9.08RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MASPGGRR29 pKa = 11.84NVLKK33 pKa = 10.49RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.13GRR40 pKa = 11.84QRR42 pKa = 11.84LVPTIYY48 pKa = 10.63KK49 pKa = 10.12KK50 pKa = 10.86

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5266

0

5266

1581447

26

3829

300.3

33.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.956 ± 0.038

1.012 ± 0.013

5.613 ± 0.024

6.223 ± 0.039

4.309 ± 0.024

7.047 ± 0.036

1.916 ± 0.019

6.048 ± 0.03

6.122 ± 0.038

10.127 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.018

3.908 ± 0.028

4.355 ± 0.024

3.568 ± 0.02

5.04 ± 0.032

7.595 ± 0.042

5.366 ± 0.046

6.418 ± 0.031

1.221 ± 0.014

2.803 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski