Variovorax sp. PBL-E5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Variovorax; unclassified Variovorax

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6867 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P2F9P9|A0A6P2F9P9_9BURK Abasic site processing protein OS=Variovorax sp. PBL-E5 OX=434014 GN=E5CHR_04246 PE=3 SV=1
MM1 pKa = 7.77SDD3 pKa = 3.0SPLYY7 pKa = 10.25GKK9 pKa = 10.41YY10 pKa = 9.97RR11 pKa = 11.84GTVVDD16 pKa = 5.31NVDD19 pKa = 3.43PLQIGRR25 pKa = 11.84IQAMVPDD32 pKa = 4.72LAGVIPGTWAMPCVPAAGINTGFFTVPMIGAGVWIEE68 pKa = 4.0FEE70 pKa = 4.81RR71 pKa = 11.84GDD73 pKa = 3.83PDD75 pKa = 3.5YY76 pKa = 10.39PIWVGGYY83 pKa = 7.96WGSAAEE89 pKa = 4.46VPTLAHH95 pKa = 6.64AVPPGVTGFVLQTPLNNGLVISDD118 pKa = 3.72VPGPTGGILIQTTTGAMISVSDD140 pKa = 3.8AGIVISNGQGAVINMTGPTVDD161 pKa = 4.49LNLGALTVVV170 pKa = 3.78

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P2FNJ9|A0A6P2FNJ9_9BURK Homoserine O-succinyltransferase OS=Variovorax sp. PBL-E5 OX=434014 GN=metX PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.71QASKK9 pKa = 9.04VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6867

0

6867

2151331

29

3309

313.3

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.256 ± 0.044

0.97 ± 0.009

5.276 ± 0.02

5.249 ± 0.027

3.593 ± 0.017

8.426 ± 0.031

2.285 ± 0.014

4.389 ± 0.02

3.17 ± 0.028

10.525 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.012

2.439 ± 0.016

5.382 ± 0.021

3.615 ± 0.019

7.177 ± 0.033

5.429 ± 0.022

5.08 ± 0.024

7.662 ± 0.025

1.409 ± 0.014

2.185 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski