Escherichia phage mEpX2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Shamshuipovirus; Escherichia virus mEpX2

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PGS0|K7PGS0_9CAUD Late gene regulator Q OS=Escherichia phage mEpX2 OX=1147154 GN=mEpX2_059 PE=3 SV=1
MM1 pKa = 7.68LSPSQSLQYY10 pKa = 10.38QKK12 pKa = 11.19EE13 pKa = 4.4SVEE16 pKa = 4.33RR17 pKa = 11.84ALTCANCGQKK27 pKa = 10.44LHH29 pKa = 5.86MLEE32 pKa = 3.97VHH34 pKa = 6.13VCSDD38 pKa = 4.12CCAEE42 pKa = 5.29LMSDD46 pKa = 4.35PNSSMYY52 pKa = 10.6EE53 pKa = 3.98EE54 pKa = 5.0EE55 pKa = 4.99DD56 pKa = 4.39DD57 pKa = 4.1EE58 pKa = 4.75

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PLN8|K7PLN8_9CAUD Uncharacterized protein OS=Escherichia phage mEpX2 OX=1147154 GN=mEpX2_014 PE=4 SV=1
MM1 pKa = 7.02QAGKK5 pKa = 9.89LRR7 pKa = 11.84HH8 pKa = 6.56RR9 pKa = 11.84ITLQEE14 pKa = 3.91PVKK17 pKa = 10.52VQNPTTGAVINTWRR31 pKa = 11.84DD32 pKa = 3.18VATVRR37 pKa = 11.84AEE39 pKa = 3.93VSPLSARR46 pKa = 11.84EE47 pKa = 3.97FIAAQASQGEE57 pKa = 4.39ITTRR61 pKa = 11.84IVIRR65 pKa = 11.84YY66 pKa = 7.05RR67 pKa = 11.84AGVTRR72 pKa = 11.84KK73 pKa = 9.73HH74 pKa = 6.46RR75 pKa = 11.84ILFRR79 pKa = 11.84GAVYY83 pKa = 10.34NIHH86 pKa = 6.74GVLPDD91 pKa = 3.77PKK93 pKa = 10.36SGRR96 pKa = 11.84EE97 pKa = 3.98YY98 pKa = 10.53LTLPCSEE105 pKa = 4.45GVNDD109 pKa = 4.16GG110 pKa = 3.76

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

11991

45

1151

179.0

19.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.465 ± 0.327

1.176 ± 0.164

5.954 ± 0.235

5.879 ± 0.285

3.327 ± 0.166

7.697 ± 0.329

1.635 ± 0.234

5.954 ± 0.204

5.971 ± 0.313

7.647 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.852 ± 0.165

4.487 ± 0.25

3.703 ± 0.2

4.67 ± 0.235

6.055 ± 0.32

6.98 ± 0.284

5.821 ± 0.36

6.263 ± 0.238

1.735 ± 0.152

2.727 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski