Nocardia seriolae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B8NSQ9|A0A0B8NSQ9_9NOCA Uncharacterized protein OS=Nocardia seriolae OX=37332 GN=NS07_v2contig00239-0003 PE=4 SV=1
MM1 pKa = 6.51TTIAEE6 pKa = 4.18YY7 pKa = 10.53LAASQIQATPVDD19 pKa = 4.14PAAPGAPKK27 pKa = 10.54VSVGVPEE34 pKa = 5.4GWQQVPPGMFPGSYY48 pKa = 10.13GVWTQPPVNGWADD61 pKa = 3.53NAVLLVATFSPAIDD75 pKa = 3.35ARR77 pKa = 11.84EE78 pKa = 4.03LLSHH82 pKa = 6.91AFDD85 pKa = 5.61DD86 pKa = 5.01ARR88 pKa = 11.84QLPGWQDD95 pKa = 3.28LDD97 pKa = 3.68SDD99 pKa = 4.15TEE101 pKa = 4.17DD102 pKa = 3.74FEE104 pKa = 5.63GYY106 pKa = 9.45PSAGITGTYY115 pKa = 10.34DD116 pKa = 3.3VAPLTLWAYY125 pKa = 9.17NRR127 pKa = 11.84YY128 pKa = 9.72IIVEE132 pKa = 4.28SEE134 pKa = 3.46NGQYY138 pKa = 10.66LVQLTITTRR147 pKa = 11.84TDD149 pKa = 3.03SDD151 pKa = 3.54GTAPTTILEE160 pKa = 4.32GLSISAA166 pKa = 4.57

Molecular weight: 17.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B8N3T5|A0A0B8N3T5_9NOCA Molybdate-binding protein OS=Nocardia seriolae OX=37332 GN=NS07_v2contig00004-0099 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.41GRR42 pKa = 11.84AKK44 pKa = 9.65LTAA47 pKa = 4.21

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7386

0

7386

2267378

37

9498

307.0

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.336 ± 0.045

0.776 ± 0.01

5.994 ± 0.022

5.415 ± 0.027

2.988 ± 0.017

9.029 ± 0.032

2.243 ± 0.016

4.2 ± 0.019

2.098 ± 0.023

10.127 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.012

2.108 ± 0.018

6.015 ± 0.03

2.851 ± 0.016

7.64 ± 0.036

5.175 ± 0.019

6.178 ± 0.022

8.308 ± 0.032

1.483 ± 0.012

2.083 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski