Klebsiella sp. RIT-PI-d

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; unclassified Klebsiella

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0AQS7|A0A0L0AQS7_9ENTR Uncharacterized protein OS=Klebsiella sp. RIT-PI-d OX=1681196 GN=AC791_16135 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.31HH3 pKa = 4.87TEE5 pKa = 3.62IRR7 pKa = 11.84SAVIDD12 pKa = 3.83ALEE15 pKa = 4.12IAVGDD20 pKa = 3.92SVIYY24 pKa = 10.37FDD26 pKa = 4.92GRR28 pKa = 11.84PAVIEE33 pKa = 4.32EE34 pKa = 4.17EE35 pKa = 4.48DD36 pKa = 4.04FPAIAVYY43 pKa = 10.56LSDD46 pKa = 4.9AEE48 pKa = 4.39YY49 pKa = 10.02TGEE52 pKa = 5.81NMDD55 pKa = 4.33CDD57 pKa = 3.52TWQATLHH64 pKa = 6.47IEE66 pKa = 3.98IFLPAQVPDD75 pKa = 4.11SEE77 pKa = 5.1LDD79 pKa = 3.09MWVEE83 pKa = 3.87SRR85 pKa = 11.84IYY87 pKa = 10.54SAISDD92 pKa = 3.76IPALARR98 pKa = 11.84LITVMVPQGYY108 pKa = 9.56EE109 pKa = 3.77YY110 pKa = 10.92QRR112 pKa = 11.84DD113 pKa = 3.65DD114 pKa = 5.51SLALWSSADD123 pKa = 3.38LKK125 pKa = 11.39YY126 pKa = 10.94SINYY130 pKa = 8.57EE131 pKa = 3.99MM132 pKa = 5.77

Molecular weight: 14.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0AX32|A0A0L0AX32_9ENTR Iron-sulfur cluster repair protein YtfE OS=Klebsiella sp. RIT-PI-d OX=1681196 GN=ytfE PE=3 SV=1
MM1 pKa = 6.32TAYY4 pKa = 9.42WLAQGVGVVAFLIGITTFINRR25 pKa = 11.84DD26 pKa = 2.87EE27 pKa = 4.45RR28 pKa = 11.84RR29 pKa = 11.84FKK31 pKa = 11.04LQLAVYY37 pKa = 8.82SAVIGLHH44 pKa = 5.79FFLMGAWPAGMSAEE58 pKa = 4.16LNAIRR63 pKa = 11.84TVISLRR69 pKa = 11.84TRR71 pKa = 11.84RR72 pKa = 11.84LWVMSVFIVLTLVLGLSKK90 pKa = 10.33LDD92 pKa = 3.65HH93 pKa = 7.04AMEE96 pKa = 5.02LLPIIGTTVSTWALFRR112 pKa = 11.84CRR114 pKa = 11.84GLTTRR119 pKa = 11.84CVMWCSTVCWVAHH132 pKa = 5.35NFWLGSIGGTLIEE145 pKa = 4.95GSFLLMNGLNIIRR158 pKa = 11.84FWRR161 pKa = 11.84MQRR164 pKa = 11.84RR165 pKa = 11.84GIDD168 pKa = 3.15PFKK171 pKa = 10.62IEE173 pKa = 4.63KK174 pKa = 9.97AVQEE178 pKa = 4.48EE179 pKa = 4.53NPSARR184 pKa = 3.72

Molecular weight: 20.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3739

0

3739

1233813

29

6598

330.0

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.819 ± 0.049

1.059 ± 0.015

5.4 ± 0.037

5.482 ± 0.044

3.802 ± 0.032

7.383 ± 0.05

2.308 ± 0.022

5.923 ± 0.031

4.112 ± 0.04

10.795 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.025

3.766 ± 0.039

4.45 ± 0.033

4.52 ± 0.029

5.693 ± 0.046

5.93 ± 0.042

5.512 ± 0.056

7.064 ± 0.038

1.518 ± 0.019

2.745 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski