Mytilus sp. clam associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL85|A0A0K1RL85_9CIRC Putative replication initiation protein OS=Mytilus sp. clam associated circular virus OX=1692256 PE=4 SV=1
MM1 pKa = 7.09SQAKK5 pKa = 9.28RR6 pKa = 11.84WCFTINNPTPAEE18 pKa = 4.03EE19 pKa = 4.14EE20 pKa = 4.49SLFLHH25 pKa = 6.61GTNIDD30 pKa = 3.97HH31 pKa = 7.24PPCDD35 pKa = 3.52FKK37 pKa = 11.76YY38 pKa = 10.65LVFGRR43 pKa = 11.84EE44 pKa = 4.05TGEE47 pKa = 3.73SGTPHH52 pKa = 5.65LQGYY56 pKa = 8.86FEE58 pKa = 4.63LVKK61 pKa = 10.49KK62 pKa = 10.62LRR64 pKa = 11.84IHH66 pKa = 6.03QIKK69 pKa = 8.42GTLGFEE75 pKa = 4.13RR76 pKa = 11.84SHH78 pKa = 8.51LEE80 pKa = 3.88VARR83 pKa = 11.84GTSLQASDD91 pKa = 3.71YY92 pKa = 10.09CKK94 pKa = 10.61KK95 pKa = 10.48EE96 pKa = 3.61NAFEE100 pKa = 4.14EE101 pKa = 5.23FGALPPPPGNAAHH114 pKa = 6.91FAQLRR119 pKa = 11.84EE120 pKa = 4.18WVAAQPSKK128 pKa = 10.04PTIKK132 pKa = 10.54DD133 pKa = 3.06VWDD136 pKa = 3.69VFPTLAARR144 pKa = 11.84YY145 pKa = 8.34NRR147 pKa = 11.84AVMEE151 pKa = 4.96CIDD154 pKa = 4.59LFGKK158 pKa = 10.56KK159 pKa = 9.1PVLVEE164 pKa = 3.58GDD166 pKa = 3.31LRR168 pKa = 11.84LWQLRR173 pKa = 11.84LDD175 pKa = 4.83GIANQEE181 pKa = 3.65ADD183 pKa = 3.63DD184 pKa = 3.91RR185 pKa = 11.84RR186 pKa = 11.84VIFVIDD192 pKa = 3.55PEE194 pKa = 4.61GNKK197 pKa = 9.95GKK199 pKa = 10.11SWLVSYY205 pKa = 9.59WLSTRR210 pKa = 11.84DD211 pKa = 3.29DD212 pKa = 3.88TQFMSVGKK220 pKa = 10.22RR221 pKa = 11.84DD222 pKa = 3.82DD223 pKa = 3.2LAYY226 pKa = 10.34AVDD229 pKa = 4.03ISTCLFVFDD238 pKa = 4.47IPRR241 pKa = 11.84GNMQYY246 pKa = 9.32IQYY249 pKa = 11.03GIFEE253 pKa = 4.15QLKK256 pKa = 8.84NRR258 pKa = 11.84VVFSNKK264 pKa = 9.43YY265 pKa = 9.35SSQTKK270 pKa = 9.71ILLNTPHH277 pKa = 6.41VFVFANEE284 pKa = 4.06LPDD287 pKa = 3.86MNALSADD294 pKa = 3.83RR295 pKa = 11.84YY296 pKa = 10.46KK297 pKa = 11.09VINII301 pKa = 3.92

Molecular weight: 34.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL85|A0A0K1RL85_9CIRC Putative replication initiation protein OS=Mytilus sp. clam associated circular virus OX=1692256 PE=4 SV=1
MM1 pKa = 7.52SGQLVRR7 pKa = 11.84SFARR11 pKa = 11.84AQYY14 pKa = 10.52PRR16 pKa = 11.84FRR18 pKa = 11.84NYY20 pKa = 10.81AATRR24 pKa = 11.84LQAAGRR30 pKa = 11.84GWLSRR35 pKa = 11.84RR36 pKa = 11.84TRR38 pKa = 11.84GAGQTFQRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84MWSGRR54 pKa = 11.84SRR56 pKa = 11.84KK57 pKa = 8.5RR58 pKa = 11.84TGQMRR63 pKa = 11.84KK64 pKa = 9.04RR65 pKa = 11.84MAVGTRR71 pKa = 11.84IGSSLAKK78 pKa = 9.99CDD80 pKa = 3.53QVEE83 pKa = 4.72DD84 pKa = 4.01NFSATTKK91 pKa = 10.37EE92 pKa = 3.9LYY94 pKa = 9.76QYY96 pKa = 11.56KK97 pKa = 10.58LLDD100 pKa = 3.59LAKK103 pKa = 10.81NNNADD108 pKa = 3.54DD109 pKa = 4.03TQVRR113 pKa = 11.84NRR115 pKa = 11.84DD116 pKa = 3.34VVNFRR121 pKa = 11.84GIKK124 pKa = 10.04LCINTTNTTTTGTIYY139 pKa = 10.46PNRR142 pKa = 11.84HH143 pKa = 4.95LHH145 pKa = 4.99CHH147 pKa = 5.19YY148 pKa = 11.06AVVSPKK154 pKa = 10.85ALDD157 pKa = 3.34ATQAVPYY164 pKa = 9.92GHH166 pKa = 7.13FFRR169 pKa = 11.84SQGEE173 pKa = 4.04VGKK176 pKa = 10.37RR177 pKa = 11.84GKK179 pKa = 10.48DD180 pKa = 3.26FGVDD184 pKa = 3.57LNGMDD189 pKa = 4.81FQCLPINTDD198 pKa = 3.47LYY200 pKa = 10.7TIHH203 pKa = 5.12QHH205 pKa = 6.43YY206 pKa = 10.22RR207 pKa = 11.84FKK209 pKa = 11.15LNKK212 pKa = 9.09AANSSTSPEE221 pKa = 3.93KK222 pKa = 10.76LYY224 pKa = 11.07EE225 pKa = 4.42KK226 pKa = 10.56YY227 pKa = 10.99LNLNRR232 pKa = 11.84QVRR235 pKa = 11.84YY236 pKa = 9.99EE237 pKa = 3.99NTGDD241 pKa = 3.48KK242 pKa = 11.02PEE244 pKa = 4.23GKK246 pKa = 9.52NVYY249 pKa = 10.25LLIWFSFIDD258 pKa = 3.98EE259 pKa = 4.68AGGTAGIPCADD270 pKa = 3.46TKK272 pKa = 11.28LRR274 pKa = 11.84ITRR277 pKa = 11.84FFKK280 pKa = 10.68EE281 pKa = 4.07SSNN284 pKa = 3.58

Molecular weight: 32.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

585

284

301

292.5

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.179 ± 0.149

1.709 ± 0.035

5.641 ± 0.493

4.615 ± 1.002

5.641 ± 0.493

6.838 ± 0.63

2.222 ± 0.076

4.444 ± 0.64

6.154 ± 0.128

8.205 ± 0.562

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.709 ± 0.035

5.812 ± 0.609

4.274 ± 1.009

4.615 ± 0.218

8.034 ± 1.753

5.47 ± 0.113

6.325 ± 1.229

5.641 ± 0.737

1.538 ± 0.334

3.932 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski