Myxococcus xanthus (strain DK1622)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Myxococcaceae; Myxococcus; Myxococcus xanthus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7314 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1CYQ9|Q1CYQ9_MYXXD Vitamin K epoxide reductase family/thioredoxin domain protein OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=MXAN_6339 PE=3 SV=1
MM1 pKa = 7.34EE2 pKa = 4.2QTAHH6 pKa = 6.35MGTNRR11 pKa = 11.84QTWLALTLTVVGLLAGCSPGASPTEE36 pKa = 3.87EE37 pKa = 4.72DD38 pKa = 3.51ADD40 pKa = 4.08GDD42 pKa = 4.42GVADD46 pKa = 4.92AADD49 pKa = 4.46CAPGDD54 pKa = 3.82ANRR57 pKa = 11.84WRR59 pKa = 11.84ILEE62 pKa = 3.87GHH64 pKa = 7.09ADD66 pKa = 3.83DD67 pKa = 6.17DD68 pKa = 4.79RR69 pKa = 11.84DD70 pKa = 4.01GVGAGALRR78 pKa = 11.84EE79 pKa = 4.26VCAGEE84 pKa = 3.86QLPEE88 pKa = 4.01GWSQQSGDD96 pKa = 4.52CAPGDD101 pKa = 3.46ATRR104 pKa = 11.84WRR106 pKa = 11.84EE107 pKa = 3.89QAGLYY112 pKa = 9.54PDD114 pKa = 3.94QDD116 pKa = 3.44NDD118 pKa = 3.61GATGEE123 pKa = 4.58GPVTACVGDD132 pKa = 3.67TLEE135 pKa = 5.28GYY137 pKa = 9.79RR138 pKa = 11.84EE139 pKa = 4.02QPGAPDD145 pKa = 4.41CDD147 pKa = 4.18DD148 pKa = 3.65NAPRR152 pKa = 11.84FAASTQLWPDD162 pKa = 3.07ADD164 pKa = 3.89FDD166 pKa = 5.14GIGNGEE172 pKa = 4.68PISHH176 pKa = 7.07CAGKK180 pKa = 10.1DD181 pKa = 3.4LPRR184 pKa = 11.84GYY186 pKa = 10.83AAVDD190 pKa = 3.92GDD192 pKa = 4.41CAPDD196 pKa = 3.39DD197 pKa = 3.95RR198 pKa = 11.84ARR200 pKa = 11.84STWRR204 pKa = 11.84DD205 pKa = 3.36YY206 pKa = 7.45THH208 pKa = 7.57RR209 pKa = 11.84DD210 pKa = 2.94ADD212 pKa = 3.77GDD214 pKa = 4.01GFTVPEE220 pKa = 4.29EE221 pKa = 4.16GRR223 pKa = 11.84VCSGMTLPAGYY234 pKa = 10.38ALEE237 pKa = 4.95ARR239 pKa = 11.84GLDD242 pKa = 4.15CDD244 pKa = 4.36DD245 pKa = 4.87TNAAWWQKK253 pKa = 7.72RR254 pKa = 11.84TVYY257 pKa = 11.17ADD259 pKa = 3.1VDD261 pKa = 3.7GDD263 pKa = 4.2GFGSGDD269 pKa = 3.12PVMRR273 pKa = 11.84CTGRR277 pKa = 11.84TAPPGYY283 pKa = 10.7ALDD286 pKa = 4.46SVDD289 pKa = 5.18CAPEE293 pKa = 4.64DD294 pKa = 3.37ATRR297 pKa = 11.84WQWRR301 pKa = 11.84SYY303 pKa = 9.37AHH305 pKa = 7.25RR306 pKa = 11.84DD307 pKa = 3.13SDD309 pKa = 4.18GDD311 pKa = 3.7GDD313 pKa = 4.22TVPEE317 pKa = 4.08QGVTCSGAALPPGYY331 pKa = 10.2AQWPTGHH338 pKa = 7.33DD339 pKa = 3.81CNDD342 pKa = 3.22RR343 pKa = 11.84DD344 pKa = 3.96ATVMVTWHH352 pKa = 6.51VYY354 pKa = 10.83SDD356 pKa = 3.96DD357 pKa = 4.24DD358 pKa = 4.46GDD360 pKa = 3.86RR361 pKa = 11.84FGAGTRR367 pKa = 11.84QTFCAGRR374 pKa = 11.84QIPVGYY380 pKa = 10.02SVVNTDD386 pKa = 4.89CDD388 pKa = 3.7DD389 pKa = 3.81TAPLLWRR396 pKa = 11.84LLAYY400 pKa = 8.0SHH402 pKa = 7.27RR403 pKa = 11.84DD404 pKa = 3.01ADD406 pKa = 4.03GDD408 pKa = 4.12SFTAPEE414 pKa = 4.19VGTVCSGMALPPGHH428 pKa = 6.71ATTPRR433 pKa = 11.84GNDD436 pKa = 3.69CDD438 pKa = 4.63DD439 pKa = 4.86ADD441 pKa = 4.18PSIHH445 pKa = 5.67TTVQAWTDD453 pKa = 3.22ADD455 pKa = 3.94GDD457 pKa = 4.41GVGAGDD463 pKa = 4.94AVALCTDD470 pKa = 4.09GTVPAPWSARR480 pKa = 11.84GTDD483 pKa = 3.94CAAEE487 pKa = 4.08DD488 pKa = 3.49AARR491 pKa = 11.84WRR493 pKa = 11.84QAAYY497 pKa = 10.53AHH499 pKa = 6.48VDD501 pKa = 3.28RR502 pKa = 11.84DD503 pKa = 4.15ADD505 pKa = 3.74GHH507 pKa = 4.83THH509 pKa = 6.93PEE511 pKa = 3.93AGTVCTGASLPEE523 pKa = 4.29PYY525 pKa = 8.66FTQATGNDD533 pKa = 4.12CDD535 pKa = 5.23DD536 pKa = 3.95SDD538 pKa = 3.72AARR541 pKa = 11.84YY542 pKa = 8.31RR543 pKa = 11.84WTVLYY548 pKa = 10.17PDD550 pKa = 3.89MDD552 pKa = 4.73GDD554 pKa = 4.72GVGTSPRR561 pKa = 11.84TIPCIGAQTPLGFSLKK577 pKa = 10.49GYY579 pKa = 10.6DD580 pKa = 4.36VDD582 pKa = 6.27DD583 pKa = 4.39SDD585 pKa = 6.46ASIQEE590 pKa = 4.24DD591 pKa = 4.26PEE593 pKa = 6.58DD594 pKa = 4.52DD595 pKa = 4.87LMLEE599 pKa = 5.14LILDD603 pKa = 4.07

Molecular weight: 63.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1CXK1|Q1CXK1_MYXXD Uncharacterized protein OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=MXAN_6755 PE=4 SV=1
MM1 pKa = 7.34TNEE4 pKa = 4.26SVNPQLTDD12 pKa = 3.58DD13 pKa = 4.37VSPSPAEE20 pKa = 4.0AGIAPTPRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84TATKK34 pKa = 9.67RR35 pKa = 11.84KK36 pKa = 8.09SASRR40 pKa = 11.84TGSTRR45 pKa = 11.84KK46 pKa = 9.4SARR49 pKa = 11.84KK50 pKa = 8.2ATAKK54 pKa = 10.4RR55 pKa = 11.84GTPRR59 pKa = 11.84AKK61 pKa = 9.24KK62 pKa = 7.44TAARR66 pKa = 11.84GKK68 pKa = 7.71TATRR72 pKa = 11.84KK73 pKa = 9.32SATRR77 pKa = 11.84KK78 pKa = 8.68SATRR82 pKa = 11.84ATGRR86 pKa = 11.84GARR89 pKa = 11.84KK90 pKa = 7.88ATPRR94 pKa = 11.84KK95 pKa = 7.83TGTAGRR101 pKa = 11.84TARR104 pKa = 11.84KK105 pKa = 7.6TGTRR109 pKa = 11.84GTATKK114 pKa = 9.5RR115 pKa = 11.84TTTGGGARR123 pKa = 11.84KK124 pKa = 9.78SPARR128 pKa = 11.84RR129 pKa = 11.84TTRR132 pKa = 11.84RR133 pKa = 3.17

Molecular weight: 14.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7314

0

7314

2775111

30

14274

379.4

41.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.228 ± 0.045

0.95 ± 0.013

5.119 ± 0.02

6.339 ± 0.032

3.305 ± 0.016

8.711 ± 0.03

2.178 ± 0.015

2.914 ± 0.022

2.81 ± 0.031

10.911 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.013

2.098 ± 0.018

6.173 ± 0.027

3.427 ± 0.02

8.168 ± 0.032

5.688 ± 0.026

5.367 ± 0.026

8.209 ± 0.03

1.362 ± 0.012

2.019 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski