Saccharicrinis fermentans DSM 9555 = JCM 21142

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Saccharicrinis; Saccharicrinis fermentans

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7YMF9|W7YMF9_9BACT Uncharacterized protein OS=Saccharicrinis fermentans DSM 9555 = JCM 21142 OX=869213 GN=JCM21142_114612 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.49KK3 pKa = 10.32LLLFLGVMVLFKK15 pKa = 10.45LTSCDD20 pKa = 4.02VLDD23 pKa = 5.35DD24 pKa = 4.87DD25 pKa = 6.07SDD27 pKa = 4.28YY28 pKa = 11.88SLDD31 pKa = 3.78NFSLSSGTVLMDD43 pKa = 3.11VDD45 pKa = 4.46SYY47 pKa = 11.62SIKK50 pKa = 9.69TDD52 pKa = 3.0NGKK55 pKa = 9.22VLWPSASNVSVSLLEE70 pKa = 4.19DD71 pKa = 3.38SMRR74 pKa = 11.84VLVNYY79 pKa = 7.63TILGEE84 pKa = 4.19ATDD87 pKa = 4.0NDD89 pKa = 4.01SYY91 pKa = 11.85DD92 pKa = 3.35YY93 pKa = 10.56YY94 pKa = 11.68VRR96 pKa = 11.84VNGLSKK102 pKa = 10.65ILTKK106 pKa = 10.39PVFEE110 pKa = 4.63FTSEE114 pKa = 3.87TTADD118 pKa = 3.93VIDD121 pKa = 5.05SIGDD125 pKa = 3.62DD126 pKa = 3.29AVTIVDD132 pKa = 3.23TWFTDD137 pKa = 3.38DD138 pKa = 3.61YY139 pKa = 11.32LNVEE143 pKa = 4.48FEE145 pKa = 4.55YY146 pKa = 11.29GGGATVHH153 pKa = 6.19YY154 pKa = 9.99INLVFDD160 pKa = 4.51AEE162 pKa = 4.35NPTTEE167 pKa = 4.6DD168 pKa = 3.1GAIILEE174 pKa = 4.5LKK176 pKa = 10.03HH177 pKa = 5.85NHH179 pKa = 6.14NGDD182 pKa = 3.81PYY184 pKa = 11.51SYY186 pKa = 9.16LQWGIASFDD195 pKa = 3.32VSAFQTAEE203 pKa = 4.0KK204 pKa = 9.8DD205 pKa = 3.59TIDD208 pKa = 3.52FFVRR212 pKa = 11.84SKK214 pKa = 11.42GKK216 pKa = 10.38DD217 pKa = 2.83GAYY220 pKa = 10.49NYY222 pKa = 10.45NQVLTYY228 pKa = 10.14SYY230 pKa = 10.94GSASVEE236 pKa = 3.64EE237 pKa = 5.06SINKK241 pKa = 9.73QYY243 pKa = 9.96DD244 pKa = 3.78TEE246 pKa = 4.36NVSLLQSIQQ255 pKa = 3.06

Molecular weight: 28.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7Y3L8|W7Y3L8_9BACT 50S ribosomal protein L3 OS=Saccharicrinis fermentans DSM 9555 = JCM 21142 OX=869213 GN=rplC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.84HH16 pKa = 3.75GFRR19 pKa = 11.84ARR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.12VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.34KK41 pKa = 10.71LSVSSEE47 pKa = 3.93KK48 pKa = 10.19RR49 pKa = 11.84HH50 pKa = 5.73KK51 pKa = 10.69AA52 pKa = 3.17

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4663

0

4663

1609379

34

4517

345.1

39.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.241 ± 0.03

0.935 ± 0.014

5.722 ± 0.026

6.433 ± 0.035

4.867 ± 0.024

6.656 ± 0.04

2.041 ± 0.018

7.569 ± 0.034

7.429 ± 0.044

9.044 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.017

5.74 ± 0.031

3.437 ± 0.02

3.503 ± 0.019

3.754 ± 0.023

6.706 ± 0.036

5.233 ± 0.033

6.461 ± 0.036

1.259 ± 0.015

4.411 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski