Beihai picorna-like virus 118

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH81|A0A1L3KH81_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 118 OX=1922547 PE=4 SV=1
MM1 pKa = 7.57SDD3 pKa = 4.1KK4 pKa = 10.8DD5 pKa = 3.42AAMPPTASGEE15 pKa = 4.04ITEE18 pKa = 4.66GMGVPPEE25 pKa = 4.13MPVDD29 pKa = 3.85AGIDD33 pKa = 3.46RR34 pKa = 11.84PGGITAEE41 pKa = 4.24VQQVHH46 pKa = 5.63TSRR49 pKa = 11.84NDD51 pKa = 3.34SIEE54 pKa = 3.88AFLRR58 pKa = 11.84SVPILQKK65 pKa = 10.13IIKK68 pKa = 9.18WSVNDD73 pKa = 3.67LPGTILYY80 pKa = 7.77QVPVHH85 pKa = 6.31PSYY88 pKa = 11.46VNYY91 pKa = 8.4MVKK94 pKa = 9.67WFKK97 pKa = 10.83KK98 pKa = 9.74AFKK101 pKa = 9.25YY102 pKa = 9.99WNGPIIFMIDD112 pKa = 3.04LAGTGFHH119 pKa = 6.53GGKK122 pKa = 10.26LIIFHH127 pKa = 6.4TPPGKK132 pKa = 10.03RR133 pKa = 11.84FKK135 pKa = 10.0PSKK138 pKa = 8.35MTMAQWFTFSHH149 pKa = 6.4GLVDD153 pKa = 5.84AKK155 pKa = 11.22SQGNNLACAPDD166 pKa = 3.86VIGRR170 pKa = 11.84TLHH173 pKa = 7.23DD174 pKa = 4.26NGPFDD179 pKa = 4.78EE180 pKa = 4.87NNEE183 pKa = 3.99DD184 pKa = 4.15TYY186 pKa = 11.73GGWFGIGVVMEE197 pKa = 5.1LKK199 pKa = 10.16TSGTGDD205 pKa = 3.36QEE207 pKa = 4.17IQFSLEE213 pKa = 3.57ACLAPSFSFNYY224 pKa = 9.55IVPTNLLEE232 pKa = 4.29EE233 pKa = 4.62DD234 pKa = 4.22EE235 pKa = 4.62EE236 pKa = 4.73VLTFDD241 pKa = 5.53LDD243 pKa = 3.8PVPILSQFPEE253 pKa = 4.06LTEE256 pKa = 4.11LRR258 pKa = 11.84FHH260 pKa = 6.74ATSAMNGNQVVYY272 pKa = 10.07LNPLGSDD279 pKa = 2.84KK280 pKa = 11.3GLLKK284 pKa = 10.71EE285 pKa = 4.72PIRR288 pKa = 11.84TLVTTTTAIQGDD300 pKa = 3.89VSVPQSQSTTKK311 pKa = 10.19PPEE314 pKa = 3.92YY315 pKa = 9.59PPTVGSGKK323 pKa = 10.22KK324 pKa = 9.51VIEE327 pKa = 4.24YY328 pKa = 7.49EE329 pKa = 4.25TDD331 pKa = 3.16SSSCGLFCPNNNNPLPINTVTLVLDD356 pKa = 4.33GGAILIAAKK365 pKa = 8.98ITDD368 pKa = 3.52NTPRR372 pKa = 11.84SGVVSLGVDD381 pKa = 3.69GALTQVRR388 pKa = 11.84HH389 pKa = 5.81NSSTSQVITQVAPNGEE405 pKa = 4.38AIITFHH411 pKa = 6.53WKK413 pKa = 10.47GDD415 pKa = 3.69TEE417 pKa = 4.18QDD419 pKa = 4.26GVCMTPGMAKK429 pKa = 9.37QCQNIDD435 pKa = 3.7PSTGTPIYY443 pKa = 8.93TLRR446 pKa = 11.84DD447 pKa = 3.37AKK449 pKa = 10.11TDD451 pKa = 3.69QVLLWVRR458 pKa = 11.84LNYY461 pKa = 10.68NGFFSTTPTNVLTRR475 pKa = 11.84ISLANKK481 pKa = 8.49VLKK484 pKa = 10.54FEE486 pKa = 4.07YY487 pKa = 10.18RR488 pKa = 11.84LAITEE493 pKa = 4.21RR494 pKa = 11.84LPIPEE499 pKa = 4.27GSEE502 pKa = 3.95LNRR505 pKa = 11.84IAAEE509 pKa = 3.8QTSFVLADD517 pKa = 4.45LKK519 pKa = 11.19QEE521 pKa = 4.09MEE523 pKa = 4.5SKK525 pKa = 10.02FRR527 pKa = 11.84NYY529 pKa = 9.13FAAFLSRR536 pKa = 11.84HH537 pKa = 5.76PMSAHH542 pKa = 5.47DD543 pKa = 4.85HH544 pKa = 6.17DD545 pKa = 4.28WKK547 pKa = 11.06DD548 pKa = 3.41FEE550 pKa = 5.5DD551 pKa = 3.75RR552 pKa = 11.84VRR554 pKa = 11.84PLDD557 pKa = 3.92TPLLDD562 pKa = 4.45PKK564 pKa = 10.17LFCATTPFSAPDD576 pKa = 3.33QASTSLGRR584 pKa = 11.84QLSDD588 pKa = 2.99IGDD591 pKa = 3.58EE592 pKa = 4.82LEE594 pKa = 4.29YY595 pKa = 11.1SNCGHH600 pKa = 7.34RR601 pKa = 11.84DD602 pKa = 3.19CKK604 pKa = 10.15YY605 pKa = 11.09AKK607 pKa = 9.86SCLKK611 pKa = 10.92AEE613 pKa = 4.22TDD615 pKa = 3.27

Molecular weight: 67.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH81|A0A1L3KH81_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 118 OX=1922547 PE=4 SV=1
MM1 pKa = 7.31AAAAAAAAGVKK12 pKa = 10.39AGGDD16 pKa = 3.75VVSTTTKK23 pKa = 10.7GLFDD27 pKa = 3.9FFQNRR32 pKa = 11.84EE33 pKa = 3.92NIDD36 pKa = 3.39YY37 pKa = 10.88KK38 pKa = 11.04KK39 pKa = 11.02FEE41 pKa = 4.58LATNQNNQLYY51 pKa = 8.09NTFLYY56 pKa = 9.57HH57 pKa = 7.05QNFFLPEE64 pKa = 4.07LEE66 pKa = 4.64LKK68 pKa = 10.44RR69 pKa = 11.84KK70 pKa = 8.53QVQSQYY76 pKa = 11.67DD77 pKa = 3.72VAALNQIEE85 pKa = 4.34SRR87 pKa = 11.84RR88 pKa = 11.84TAQQLSAYY96 pKa = 9.49NRR98 pKa = 11.84EE99 pKa = 3.93TALQLGQQNFSNALKK114 pKa = 10.63LGNNQFEE121 pKa = 4.42QSRR124 pKa = 11.84KK125 pKa = 9.94SMQLATKK132 pKa = 10.3LNLEE136 pKa = 4.6ADD138 pKa = 3.42VSAYY142 pKa = 7.79QRR144 pKa = 11.84KK145 pKa = 8.43LATQTNVLNRR155 pKa = 11.84DD156 pKa = 3.74GLPSSLAILGAGAPRR171 pKa = 11.84EE172 pKa = 3.88RR173 pKa = 11.84IYY175 pKa = 11.19AGNRR179 pKa = 11.84TFVSAYY185 pKa = 9.44QGNGPAYY192 pKa = 10.43SSTPSQMMLGNIKK205 pKa = 10.49LEE207 pKa = 4.38PKK209 pKa = 10.38

Molecular weight: 23.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2837

209

2013

945.7

106.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.768 ± 1.57

1.692 ± 0.479

6.591 ± 1.063

5.675 ± 0.303

4.935 ± 0.113

5.746 ± 0.737

2.82 ± 0.769

5.816 ± 0.968

6.768 ± 0.866

9.059 ± 0.571

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.151

4.406 ± 1.358

5.746 ± 0.988

3.913 ± 1.582

4.582 ± 0.531

7.191 ± 0.175

6.838 ± 0.679

5.146 ± 0.604

1.128 ± 0.314

3.243 ± 0.432
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski