Streptococcus phage IPP5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S7N3|A0A1S5S7N3_9CAUD Portal protein OS=Streptococcus phage IPP5 OX=1916187 GN=IPP5_00030 PE=4 SV=1
MM1 pKa = 7.34MEE3 pKa = 4.11EE4 pKa = 4.75LKK6 pKa = 10.89QKK8 pKa = 10.51VNAVYY13 pKa = 10.45NWTVEE18 pKa = 4.22DD19 pKa = 4.87GKK21 pKa = 9.82PQPPQQDD28 pKa = 3.22LPQTVKK34 pKa = 11.08DD35 pKa = 3.45RR36 pKa = 11.84VDD38 pKa = 3.92YY39 pKa = 9.5FWEE42 pKa = 4.0MAEE45 pKa = 5.33DD46 pKa = 3.88GMTFMGAMEE55 pKa = 5.67CIFADD60 pKa = 4.73EE61 pKa = 5.02KK62 pKa = 10.3PTDD65 pKa = 3.84YY66 pKa = 11.54DD67 pKa = 3.97LGATKK72 pKa = 10.44GWLPKK77 pKa = 10.56SKK79 pKa = 10.58EE80 pKa = 3.54FDD82 pKa = 3.25DD83 pKa = 3.91WVGYY87 pKa = 8.92SPSMAQVVIAVYY99 pKa = 10.36LIYY102 pKa = 10.64RR103 pKa = 11.84GNN105 pKa = 3.24

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S7M1|A0A1S5S7M1_9CAUD Uncharacterized protein OS=Streptococcus phage IPP5 OX=1916187 GN=IPP5_00018 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.18PYY8 pKa = 10.05SGKK11 pKa = 10.15KK12 pKa = 9.81EE13 pKa = 4.19STFVKK18 pKa = 10.19ADD20 pKa = 3.42PEE22 pKa = 4.15IVEE25 pKa = 4.52KK26 pKa = 10.56ILRR29 pKa = 11.84NTSFVEE35 pKa = 3.87RR36 pKa = 11.84LQVLLATKK44 pKa = 10.25LL45 pKa = 3.53

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10201

38

2153

217.0

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.588 ± 0.613

0.559 ± 0.102

6.029 ± 0.327

8.058 ± 0.393

3.784 ± 0.191

5.627 ± 0.412

1.441 ± 0.187

6.784 ± 0.252

8.519 ± 0.391

8.646 ± 0.431

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.247

5.892 ± 0.35

2.725 ± 0.312

4.205 ± 0.208

4.833 ± 0.338

6.362 ± 0.549

6.627 ± 0.396

5.95 ± 0.197

1.333 ± 0.159

3.725 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski