Escherichia phage RCS47

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Punavirus; Escherichia virus RCS47

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077SK30|A0A077SK30_9CAUD Maturation control protein OS=Escherichia phage RCS47 OX=1590550 GN=mat PE=4 SV=1
MM1 pKa = 7.64AFIQPTIDD9 pKa = 4.42DD10 pKa = 3.8VRR12 pKa = 11.84HH13 pKa = 6.05CSNALSVDD21 pKa = 3.55PAEE24 pKa = 4.25TDD26 pKa = 3.0AARR29 pKa = 11.84AIAEE33 pKa = 4.36HH34 pKa = 6.3YY35 pKa = 10.88SKK37 pKa = 10.62ISNQEE42 pKa = 3.46YY43 pKa = 10.42RR44 pKa = 11.84ITQDD48 pKa = 4.89DD49 pKa = 4.74LDD51 pKa = 5.05DD52 pKa = 4.06LTDD55 pKa = 4.01TIEE58 pKa = 4.42YY59 pKa = 10.97LMATNQLDD67 pKa = 3.87SQQ69 pKa = 4.02

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077SLN8|A0A077SLN8_9CAUD Tail sheath protein Gp22 OS=Escherichia phage RCS47 OX=1590550 GN=22 PE=4 SV=1
MM1 pKa = 7.57ASVSISCPSCSATDD15 pKa = 3.7GVVRR19 pKa = 11.84NGKK22 pKa = 8.27STAGHH27 pKa = 4.88QRR29 pKa = 11.84YY30 pKa = 8.94LCSHH34 pKa = 6.46CRR36 pKa = 11.84KK37 pKa = 7.49TWQLQFTYY45 pKa = 9.73TASQPGTHH53 pKa = 5.79QKK55 pKa = 10.33IIDD58 pKa = 3.52MAMNGVGCRR67 pKa = 11.84ATARR71 pKa = 11.84IMGVGLNTIFRR82 pKa = 11.84HH83 pKa = 5.05LKK85 pKa = 9.21KK86 pKa = 10.41LRR88 pKa = 11.84PQSVTSRR95 pKa = 11.84IQPGSDD101 pKa = 3.24VIVCAEE107 pKa = 4.04MDD109 pKa = 3.97EE110 pKa = 4.08QWGYY114 pKa = 11.45VGAKK118 pKa = 9.33SRR120 pKa = 11.84QRR122 pKa = 11.84WLFYY126 pKa = 10.59AYY128 pKa = 10.32DD129 pKa = 3.89RR130 pKa = 11.84LRR132 pKa = 11.84KK133 pKa = 7.56TVVAHH138 pKa = 5.58VFGEE142 pKa = 4.23RR143 pKa = 11.84TMATLGRR150 pKa = 11.84LMSLLSPFDD159 pKa = 3.51VVIWMTDD166 pKa = 2.56GWPLYY171 pKa = 9.76EE172 pKa = 4.63SRR174 pKa = 11.84LKK176 pKa = 11.14GKK178 pKa = 9.61LHH180 pKa = 6.35VISKK184 pKa = 10.15RR185 pKa = 11.84YY186 pKa = 4.5TQRR189 pKa = 11.84IEE191 pKa = 3.79RR192 pKa = 11.84HH193 pKa = 5.1NLNLRR198 pKa = 11.84QHH200 pKa = 6.9LARR203 pKa = 11.84LGRR206 pKa = 11.84KK207 pKa = 8.76SLSFSKK213 pKa = 10.61SVEE216 pKa = 3.91LHH218 pKa = 6.59DD219 pKa = 5.11KK220 pKa = 11.16VIGHH224 pKa = 6.18YY225 pKa = 10.87LNIKK229 pKa = 9.58HH230 pKa = 5.41YY231 pKa = 9.45QQ232 pKa = 3.14

Molecular weight: 26.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

122

0

122

33425

31

2255

274.0

30.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.699 ± 0.256

1.203 ± 0.117

5.789 ± 0.194

6.516 ± 0.209

3.458 ± 0.119

6.827 ± 0.205

1.903 ± 0.118

5.783 ± 0.129

5.631 ± 0.228

8.802 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.104

4.476 ± 0.136

3.973 ± 0.128

4.227 ± 0.136

6.142 ± 0.209

6.39 ± 0.167

5.81 ± 0.254

6.405 ± 0.148

1.328 ± 0.084

3.034 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski