Nitrospirales bacterium LBB_01

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; unclassified Nitrospirales

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7X592|A0A4Y7X592_9BACT Uncharacterized protein OS=Nitrospirales bacterium LBB_01 OX=2552947 GN=E2O03_06600 PE=4 SV=1
MM1 pKa = 7.63IKK3 pKa = 10.49LGDD6 pKa = 3.35ITLPNDD12 pKa = 3.86LTWPNCFEE20 pKa = 3.86WTGISEE26 pKa = 4.53TVEE29 pKa = 3.91RR30 pKa = 11.84TLSGDD35 pKa = 3.39VVIYY39 pKa = 8.8GTEE42 pKa = 3.56ITGRR46 pKa = 11.84TIDD49 pKa = 3.57LTGGSDD55 pKa = 3.87YY56 pKa = 11.66GWITYY61 pKa = 10.3KK62 pKa = 10.6DD63 pKa = 4.15LKK65 pKa = 9.92TLLDD69 pKa = 3.51MASVYY74 pKa = 10.77EE75 pKa = 4.26EE76 pKa = 4.56IYY78 pKa = 11.1

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7X511|A0A4Y7X511_9BACT Glycosyltransferase family 1 protein (Fragment) OS=Nitrospirales bacterium LBB_01 OX=2552947 GN=E2O03_05995 PE=4 SV=1
MM1 pKa = 7.47GNVKK5 pKa = 9.7KK6 pKa = 9.58WRR8 pKa = 11.84KK9 pKa = 9.84KK10 pKa = 9.99KK11 pKa = 9.66MSKK14 pKa = 10.02HH15 pKa = 3.98KK16 pKa = 10.12HH17 pKa = 4.57KK18 pKa = 10.73KK19 pKa = 9.25LRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.1MKK25 pKa = 8.64FQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 9.33

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2708

0

2708

637417

18

1274

235.4

26.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.169 ± 0.053

1.127 ± 0.025

5.506 ± 0.037

6.386 ± 0.051

4.656 ± 0.047

6.486 ± 0.048

1.908 ± 0.023

8.089 ± 0.043

7.337 ± 0.048

9.651 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.023

4.392 ± 0.032

3.592 ± 0.032

2.6 ± 0.026

4.279 ± 0.034

6.784 ± 0.048

5.82 ± 0.041

7.08 ± 0.041

0.838 ± 0.018

3.581 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski