Bacillus phage Gxv1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Picovirinae; Salasvirus; Bacillus virus Gxv1

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z7E5|A0A6M9Z7E5_9CAUD Morphogenesis protein OS=Bacillus phage Gxv1 OX=2736266 PE=4 SV=1
MM1 pKa = 6.55VQNDD5 pKa = 3.87FVDD8 pKa = 4.13SYY10 pKa = 11.74DD11 pKa = 3.58VTMLLQDD18 pKa = 4.71DD19 pKa = 4.87DD20 pKa = 4.15GKK22 pKa = 11.02QYY24 pKa = 11.3YY25 pKa = 9.11EE26 pKa = 3.93YY27 pKa = 10.81HH28 pKa = 6.64KK29 pKa = 10.8GLSLSDD35 pKa = 3.86FEE37 pKa = 4.66VLYY40 pKa = 11.22GNTADD45 pKa = 4.77EE46 pKa = 4.67IIKK49 pKa = 10.58LRR51 pKa = 11.84LDD53 pKa = 3.13KK54 pKa = 10.79MLL56 pKa = 4.73

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z6Y1|A0A6M9Z6Y1_9CAUD Head morphogensis protein OS=Bacillus phage Gxv1 OX=2736266 PE=4 SV=1
MM1 pKa = 7.18SRR3 pKa = 11.84LWKK6 pKa = 10.36SISFSITYY14 pKa = 10.26RR15 pKa = 11.84GGNKK19 pKa = 8.89MNQKK23 pKa = 9.48EE24 pKa = 4.21FQAVLDD30 pKa = 3.7WMLSHH35 pKa = 7.45TIIQFHH41 pKa = 7.02EE42 pKa = 4.22YY43 pKa = 10.86NYY45 pKa = 9.75MLQKK49 pKa = 10.53SLPFLRR55 pKa = 11.84RR56 pKa = 3.17

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

6740

53

854

204.2

23.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.475 ± 0.367

0.801 ± 0.171

6.365 ± 0.332

6.513 ± 0.42

4.347 ± 0.371

6.558 ± 0.757

1.484 ± 0.124

6.766 ± 0.271

7.418 ± 0.48

8.027 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.245

6.78 ± 0.516

2.745 ± 0.176

3.591 ± 0.292

4.362 ± 0.309

6.884 ± 0.421

6.439 ± 0.477

6.914 ± 0.462

1.202 ± 0.207

4.525 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski