Synechococcus phage S-EIVl

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4K413|A0A0C4K413_9VIRU Uncharacterized protein OS=Synechococcus phage S-EIVl OX=1468179 PE=4 SV=1
MM1 pKa = 7.55TEE3 pKa = 4.0HH4 pKa = 7.06TDD6 pKa = 3.52EE7 pKa = 4.45QLLAMAMANLGEE19 pKa = 4.35YY20 pKa = 9.48IHH22 pKa = 7.37DD23 pKa = 4.31GSLHH27 pKa = 6.08YY28 pKa = 10.49ILIEE32 pKa = 3.88EE33 pKa = 4.62DD34 pKa = 3.2PRR36 pKa = 11.84NDD38 pKa = 3.13EE39 pKa = 6.4DD40 pKa = 4.9YY41 pKa = 9.83DD42 pKa = 3.58TWEE45 pKa = 4.12VGMEE49 pKa = 4.42PLPGDD54 pKa = 3.92HH55 pKa = 6.68TWRR58 pKa = 11.84AASIDD63 pKa = 3.77VAVSPTEE70 pKa = 4.01STDD73 pKa = 2.98

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4K424|A0A0C4K424_9VIRU Uncharacterized protein OS=Synechococcus phage S-EIVl OX=1468179 PE=4 SV=1
MM1 pKa = 7.39NKK3 pKa = 9.46VKK5 pKa = 9.72TLTQKK10 pKa = 10.96KK11 pKa = 8.54EE12 pKa = 3.82GRR14 pKa = 11.84PKK16 pKa = 9.97GTRR19 pKa = 11.84QGQGMSSKK27 pKa = 10.09PNHH30 pKa = 5.63GRR32 pKa = 11.84KK33 pKa = 7.91KK34 pKa = 9.21TRR36 pKa = 11.84GQGKK40 pKa = 9.23GG41 pKa = 3.16

Molecular weight: 4.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

23154

29

901

179.5

19.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.292 ± 0.361

1.037 ± 0.11

5.818 ± 0.234

6.37 ± 0.334

3.779 ± 0.159

8.12 ± 0.412

1.468 ± 0.148

5.23 ± 0.202

5.727 ± 0.355

8.15 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.165

4.604 ± 0.172

4.846 ± 0.25

4.872 ± 0.205

4.846 ± 0.235

6.457 ± 0.225

7.126 ± 0.569

5.783 ± 0.248

1.386 ± 0.124

3.589 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski