Blastococcus sp. DSM 44272

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238UNR6|A0A238UNR6_9ACTN Homogentisate 1 2-dioxygenase OS=Blastococcus sp. DSM 44272 OX=1938746 GN=SAMN06272737_101157 PE=3 SV=1
MM1 pKa = 7.31FLLRR5 pKa = 11.84PALVPLASVAVAVLLAGCGAQVEE28 pKa = 4.79DD29 pKa = 4.78PDD31 pKa = 4.65AAASPDD37 pKa = 3.58AAGTSAACDD46 pKa = 3.79DD47 pKa = 4.87PDD49 pKa = 4.07ISGDD53 pKa = 3.45PFAAPEE59 pKa = 4.09LTEE62 pKa = 5.09LEE64 pKa = 4.57ADD66 pKa = 3.74TEE68 pKa = 4.42LAGQVPDD75 pKa = 4.27TYY77 pKa = 10.84TEE79 pKa = 3.91QPLVIGVAPDD89 pKa = 3.88LPPINFGGEE98 pKa = 3.9DD99 pKa = 3.88GEE101 pKa = 4.4QRR103 pKa = 11.84GYY105 pKa = 10.77EE106 pKa = 4.01ADD108 pKa = 3.69LLRR111 pKa = 11.84AAGQLVGVEE120 pKa = 4.33IVFQEE125 pKa = 4.3SQNALQEE132 pKa = 4.2YY133 pKa = 10.3SAGQVDD139 pKa = 5.7GIAGAFTDD147 pKa = 3.46TLEE150 pKa = 4.11RR151 pKa = 11.84QKK153 pKa = 11.18LGDD156 pKa = 4.34FIDD159 pKa = 4.03YY160 pKa = 8.63STGSRR165 pKa = 11.84AALTQKK171 pKa = 10.33CNPKK175 pKa = 10.36GIEE178 pKa = 4.13STEE181 pKa = 4.07DD182 pKa = 3.23LCGLEE187 pKa = 4.24VVAAVGTVQLAQLTDD202 pKa = 3.54SEE204 pKa = 5.24TPGSLLTLCEE214 pKa = 4.18EE215 pKa = 4.84AGLPAPIPVQADD227 pKa = 3.6TSVSAITSLTAGRR240 pKa = 11.84AAAMVTGEE248 pKa = 4.55PIAVNAVKK256 pKa = 10.4QSEE259 pKa = 4.42GALTIAYY266 pKa = 9.81VEE268 pKa = 4.25EE269 pKa = 4.63LPVPVGVMISKK280 pKa = 9.25EE281 pKa = 4.16HH282 pKa = 6.79AEE284 pKa = 4.07LTAVLAQAYY293 pKa = 8.41QKK295 pKa = 11.05LVDD298 pKa = 4.57DD299 pKa = 4.4GTYY302 pKa = 10.85AEE304 pKa = 4.77IMNAYY309 pKa = 9.04GIEE312 pKa = 4.04YY313 pKa = 10.43GLLDD317 pKa = 4.63TITINGATSS326 pKa = 2.88

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238ZQI6|A0A238ZQI6_9ACTN Methyltransferase domain-containing protein OS=Blastococcus sp. DSM 44272 OX=1938746 GN=SAMN06272737_1322 PE=4 SV=1
MM1 pKa = 7.4GALPARR7 pKa = 11.84PLPVGPLPVPQLPAEE22 pKa = 4.42PLPAVPLPAVPLPAVPLPAVPLPAVPLPAVPLPVVHH58 pKa = 7.37RR59 pKa = 11.84LRR61 pKa = 11.84ARR63 pKa = 11.84GRR65 pKa = 11.84VRR67 pKa = 11.84ARR69 pKa = 11.84RR70 pKa = 11.84PVRR73 pKa = 11.84ATTPSAARR81 pKa = 11.84HH82 pKa = 4.57ARR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84PVARR90 pKa = 11.84LAPVRR95 pKa = 11.84PHH97 pKa = 6.73PALLARR103 pKa = 11.84VRR105 pKa = 11.84RR106 pKa = 11.84PAAPARR112 pKa = 11.84VRR114 pKa = 11.84ADD116 pKa = 3.69LVPLRR121 pKa = 11.84VPVVRR126 pKa = 11.84VRR128 pKa = 11.84LPVPAVPARR137 pKa = 11.84LPVPVVRR144 pKa = 11.84VPTRR148 pKa = 11.84GG149 pKa = 3.08

Molecular weight: 15.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4923

0

4923

1496879

25

2014

304.1

32.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.008 ± 0.054

0.726 ± 0.009

6.155 ± 0.027

5.629 ± 0.035

2.613 ± 0.019

9.529 ± 0.037

2.075 ± 0.016

3.088 ± 0.023

1.471 ± 0.019

10.616 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.705 ± 0.011

1.535 ± 0.015

6.197 ± 0.029

2.746 ± 0.02

8.22 ± 0.041

4.988 ± 0.022

5.915 ± 0.024

9.514 ± 0.039

1.467 ± 0.015

1.803 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski