Pea enation mosaic virus-2 (PEMV-2)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q84694|Q84694_PEMV2 RNA-directed RNA polymerase (Fragment) OS=Pea enation mosaic virus-2 OX=193120 PE=2 SV=1
MM1 pKa = 7.2NCVARR6 pKa = 11.84IKK8 pKa = 10.5RR9 pKa = 11.84WFTPDD14 pKa = 3.54FTPGEE19 pKa = 4.44GVKK22 pKa = 10.42SRR24 pKa = 11.84AQLEE28 pKa = 4.46RR29 pKa = 11.84EE30 pKa = 4.44LDD32 pKa = 3.76PTWALLVCQEE42 pKa = 4.1RR43 pKa = 11.84ARR45 pKa = 11.84RR46 pKa = 11.84DD47 pKa = 3.18ADD49 pKa = 3.79SIANEE54 pKa = 4.02WYY56 pKa = 9.57EE57 pKa = 4.82GSMEE61 pKa = 4.51CNLLIPRR68 pKa = 11.84PTTEE72 pKa = 4.82DD73 pKa = 3.32VFGPSIAPEE82 pKa = 3.73PVALVEE88 pKa = 3.89EE89 pKa = 4.92TTRR92 pKa = 11.84SRR94 pKa = 11.84APCVDD99 pKa = 3.27VPAEE103 pKa = 4.13EE104 pKa = 4.44SCKK107 pKa = 10.45SAEE110 pKa = 4.1IDD112 pKa = 3.7PVDD115 pKa = 3.64LAKK118 pKa = 10.36FDD120 pKa = 3.88SLHH123 pKa = 6.36RR124 pKa = 11.84RR125 pKa = 11.84LLAEE129 pKa = 4.48ANPCRR134 pKa = 11.84EE135 pKa = 4.0MVLWVPPGLPAEE147 pKa = 4.22RR148 pKa = 11.84DD149 pKa = 3.54VLPRR153 pKa = 11.84ARR155 pKa = 11.84GVIMIPEE162 pKa = 4.43VPASAHH168 pKa = 4.81TLSVKK173 pKa = 10.31VMEE176 pKa = 4.68AVRR179 pKa = 11.84LAQEE183 pKa = 4.31VLASLAKK190 pKa = 9.94RR191 pKa = 11.84ALEE194 pKa = 4.01KK195 pKa = 10.66RR196 pKa = 11.84STPTLTAQAQPEE208 pKa = 4.27ATLSGCDD215 pKa = 3.33YY216 pKa = 10.53PYY218 pKa = 11.0QEE220 pKa = 4.71TGAAAAWITPGCIAMEE236 pKa = 4.24LRR238 pKa = 11.84AKK240 pKa = 10.67FGVCKK245 pKa = 9.71RR246 pKa = 11.84TPANLEE252 pKa = 3.58MGSRR256 pKa = 11.84VARR259 pKa = 11.84EE260 pKa = 4.06LLRR263 pKa = 11.84DD264 pKa = 3.42NCVTCRR270 pKa = 11.84EE271 pKa = 4.47TTWYY275 pKa = 9.21TSAIAVDD282 pKa = 4.23LWLTPTVVDD291 pKa = 4.33LACGRR296 pKa = 11.84RR297 pKa = 11.84AADD300 pKa = 3.98FWW302 pKa = 5.89

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q84696|Q84696_PEMV2 27kDa protein OS=Pea enation mosaic virus-2 OX=193120 PE=2 SV=1
MM1 pKa = 7.66AVGKK5 pKa = 10.66YY6 pKa = 6.42MTIIINVNNDD16 pKa = 2.65EE17 pKa = 4.23RR18 pKa = 11.84KK19 pKa = 9.36QPEE22 pKa = 4.32GATGSSVRR30 pKa = 11.84RR31 pKa = 11.84GDD33 pKa = 3.53NKK35 pKa = 9.44RR36 pKa = 11.84TRR38 pKa = 11.84GNKK41 pKa = 8.59PRR43 pKa = 11.84SHH45 pKa = 6.71HH46 pKa = 5.96PGSRR50 pKa = 11.84EE51 pKa = 3.51RR52 pKa = 11.84KK53 pKa = 8.9GYY55 pKa = 9.09NHH57 pKa = 7.64PSPTPKK63 pKa = 10.09NSKK66 pKa = 9.6QGQLRR71 pKa = 11.84TEE73 pKa = 4.48AVQEE77 pKa = 4.25HH78 pKa = 7.11PKK80 pKa = 10.66HH81 pKa = 6.2GGTAFRR87 pKa = 11.84RR88 pKa = 11.84EE89 pKa = 4.5SGGSVHH95 pKa = 7.01PSHH98 pKa = 7.17PRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84ARR104 pKa = 11.84RR105 pKa = 11.84GGDD108 pKa = 3.06MAPRR112 pKa = 11.84QHH114 pKa = 6.96PPPPRR119 pKa = 11.84EE120 pKa = 3.54RR121 pKa = 11.84RR122 pKa = 11.84TKK124 pKa = 10.5TEE126 pKa = 3.69TQAEE130 pKa = 4.04RR131 pKa = 11.84RR132 pKa = 11.84AQALSVLPTLLDD144 pKa = 4.38CIGGLDD150 pKa = 3.94LGPAEE155 pKa = 4.56VLLHH159 pKa = 5.4CHH161 pKa = 5.7RR162 pKa = 11.84AVRR165 pKa = 11.84RR166 pKa = 11.84QLRR169 pKa = 11.84TGVQPIQPVPHH180 pKa = 6.27VEE182 pKa = 4.12STHH185 pKa = 6.73RR186 pKa = 11.84SSDD189 pKa = 3.56PQLLEE194 pKa = 4.23SSTTCSANLQNDD206 pKa = 3.98GAGRR210 pKa = 11.84VIGGGIPTAIPEE222 pKa = 4.52GSDD225 pKa = 3.41VEE227 pKa = 4.66QVCHH231 pKa = 6.42ASHH234 pKa = 6.62YY235 pKa = 10.39GG236 pKa = 3.23

Molecular weight: 25.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1369

236

581

342.3

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.889 ± 1.406

2.63 ± 0.486

4.383 ± 0.626

5.771 ± 1.039

3.214 ± 1.031

6.866 ± 1.024

2.557 ± 0.839

3.871 ± 0.257

4.748 ± 0.731

8.327 ± 0.958

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.293

3.579 ± 0.617

8.035 ± 0.473

3.287 ± 0.559

7.962 ± 1.103

6.939 ± 0.823

5.844 ± 0.714

7.962 ± 0.618

1.461 ± 0.447

2.337 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski