archaeon HR02

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1939 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5V324|A0A2H5V324_9ARCH Trehalose transport system permease protein SugB OS=archaeon HR02 OX=2035438 GN=sugB_1 PE=3 SV=1
MM1 pKa = 7.71NITDD5 pKa = 4.08VDD7 pKa = 3.9DD8 pKa = 5.43KK9 pKa = 11.32IIDD12 pKa = 3.53RR13 pKa = 11.84AAEE16 pKa = 3.87EE17 pKa = 4.42GVARR21 pKa = 11.84INIIYY26 pKa = 9.91LYY28 pKa = 8.98EE29 pKa = 3.89QIYY32 pKa = 10.35LYY34 pKa = 9.85EE35 pKa = 3.99QQLII39 pKa = 3.88

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5V141|A0A2H5V141_9ARCH Uncharacterized protein OS=archaeon HR02 OX=2035438 GN=HRbin02_00191 PE=3 SV=1
MM1 pKa = 7.72EE2 pKa = 4.68NQSFICLPMVNPPGDD17 pKa = 3.57LTGGDD22 pKa = 3.3GRR24 pKa = 11.84MMVRR28 pKa = 11.84LTTLPDD34 pKa = 3.5EE35 pKa = 4.79PRR37 pKa = 11.84GGAWPSPVRR46 pKa = 11.84AVRR49 pKa = 11.84CPVNSGNGRR58 pKa = 11.84DD59 pKa = 4.16PRR61 pKa = 11.84PLLPSAEE68 pKa = 4.13RR69 pKa = 11.84RR70 pKa = 11.84WALRR74 pKa = 11.84GDD76 pKa = 3.69RR77 pKa = 11.84PRR79 pKa = 11.84KK80 pKa = 8.93GEE82 pKa = 3.73EE83 pKa = 3.73GAGYY87 pKa = 9.88GRR89 pKa = 11.84SVCPEE94 pKa = 3.84SPGPHH99 pKa = 5.42ATFNGGDD106 pKa = 3.37SGTPPRR112 pKa = 11.84KK113 pKa = 10.11GEE115 pKa = 4.66LIPKK119 pKa = 9.78LRR121 pKa = 11.84LSWNRR126 pKa = 11.84GLQLALVNAEE136 pKa = 4.14SLVTARR142 pKa = 11.84YY143 pKa = 8.49HH144 pKa = 6.08RR145 pKa = 11.84AVNTSPLLAHH155 pKa = 6.34TAHH158 pKa = 7.36RR159 pKa = 11.84STRR162 pKa = 11.84AGDD165 pKa = 3.32GRR167 pKa = 11.84EE168 pKa = 3.9PLKK171 pKa = 11.17LGGPSLDD178 pKa = 3.38PVRR181 pKa = 11.84GEE183 pKa = 4.04KK184 pKa = 10.42LL185 pKa = 3.18

Molecular weight: 19.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1939

0

1939

506736

29

1589

261.3

29.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.092 ± 0.055

0.944 ± 0.021

4.382 ± 0.034

7.256 ± 0.056

3.579 ± 0.04

7.94 ± 0.048

1.722 ± 0.02

6.867 ± 0.047

4.685 ± 0.052

10.917 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.025

2.576 ± 0.031

4.662 ± 0.033

2.21 ± 0.027

7.468 ± 0.057

6.432 ± 0.042

4.637 ± 0.039

8.471 ± 0.046

1.27 ± 0.024

3.533 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski