Trueperella bernardiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Trueperella

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W1KKY7|A0A0W1KKY7_9ACTO Putative conjugal transfer protein OS=Trueperella bernardiae OX=59561 GN=AQZ59_00623 PE=3 SV=1
MM1 pKa = 7.34SLCAAASLLVACSSGGQNAGPSTSPAPEE29 pKa = 3.94PTNVAGAVAIQAEE42 pKa = 4.88MPAAPAGLEE51 pKa = 3.81EE52 pKa = 5.55FYY54 pKa = 10.98DD55 pKa = 4.98QEE57 pKa = 4.74VEE59 pKa = 3.84WSACGSGFEE68 pKa = 4.45CAEE71 pKa = 3.77ATVPLDD77 pKa = 3.36YY78 pKa = 10.99DD79 pKa = 3.63NPGGKK84 pKa = 8.4TITISMKK91 pKa = 10.73KK92 pKa = 8.98MLATGDD98 pKa = 4.02PIGTLFVNPGGPGGSALDD116 pKa = 3.85MVDD119 pKa = 3.17QANLYY124 pKa = 10.67FSDD127 pKa = 4.06QILANFDD134 pKa = 3.21IVGADD139 pKa = 3.33PRR141 pKa = 11.84GVGEE145 pKa = 4.34STPVDD150 pKa = 3.97CLDD153 pKa = 4.48DD154 pKa = 5.08ADD156 pKa = 4.18LAAYY160 pKa = 10.52LDD162 pKa = 3.72TSYY165 pKa = 11.46PDD167 pKa = 3.58TPEE170 pKa = 3.98GKK172 pKa = 9.93AQAKK176 pKa = 9.92VDD178 pKa = 3.89TEE180 pKa = 4.18KK181 pKa = 11.14LVAGCKK187 pKa = 10.11DD188 pKa = 2.95KK189 pKa = 11.67SGDD192 pKa = 3.52LLEE195 pKa = 4.61FVGTRR200 pKa = 11.84SAAKK204 pKa = 10.29DD205 pKa = 3.68LDD207 pKa = 3.65VLRR210 pKa = 11.84QVVGDD215 pKa = 3.6PKK217 pKa = 10.59LYY219 pKa = 10.76YY220 pKa = 10.66VGFSYY225 pKa = 8.94GTSLGGMYY233 pKa = 10.73AEE235 pKa = 5.2LFPGNVGRR243 pKa = 11.84MILDD247 pKa = 3.67GAVDD251 pKa = 4.03DD252 pKa = 5.49SISSFDD258 pKa = 3.34QAKK261 pKa = 9.37AQVVGFTEE269 pKa = 4.98AFDD272 pKa = 5.5AYY274 pKa = 10.47LQHH277 pKa = 6.56CVEE280 pKa = 4.67GGKK283 pKa = 9.97CALGATVDD291 pKa = 3.81EE292 pKa = 5.3GWAKK296 pKa = 10.4LAEE299 pKa = 4.28LTDD302 pKa = 3.83QIKK305 pKa = 10.14EE306 pKa = 4.21KK307 pKa = 10.17PVSAGSGRR315 pKa = 11.84VLGEE319 pKa = 3.51TGFFYY324 pKa = 10.89GIITPLYY331 pKa = 10.55DD332 pKa = 4.24DD333 pKa = 4.91SLWFALDD340 pKa = 3.42AGFDD344 pKa = 3.93SLIHH348 pKa = 7.32DD349 pKa = 4.72DD350 pKa = 4.71DD351 pKa = 5.88GSIFALLFDD360 pKa = 3.77QYY362 pKa = 10.64MGRR365 pKa = 11.84EE366 pKa = 3.61GDD368 pKa = 3.54KK369 pKa = 10.61FVNNMFEE376 pKa = 4.47ANFAISCADD385 pKa = 3.2THH387 pKa = 7.95VEE389 pKa = 3.95GTEE392 pKa = 4.01ADD394 pKa = 3.58WDD396 pKa = 4.02RR397 pKa = 11.84MSDD400 pKa = 3.53EE401 pKa = 4.81LKK403 pKa = 10.57EE404 pKa = 3.9ISPVFGAAMGYY415 pKa = 9.16SEE417 pKa = 5.03YY418 pKa = 10.88ACQIMPGGEE427 pKa = 4.19DD428 pKa = 3.42GSLGPFVAAGSDD440 pKa = 3.8PIVVVGTTGDD450 pKa = 3.51PATPYY455 pKa = 10.21EE456 pKa = 4.09WGKK459 pKa = 10.94AFADD463 pKa = 3.42NLEE466 pKa = 4.0NSRR469 pKa = 11.84FVTWEE474 pKa = 4.26GEE476 pKa = 3.85GHH478 pKa = 5.33TAYY481 pKa = 10.7SRR483 pKa = 11.84AGEE486 pKa = 4.91CISQPLDD493 pKa = 3.15QYY495 pKa = 11.49LLTGEE500 pKa = 4.32APEE503 pKa = 6.05DD504 pKa = 4.06GLTCPAEE511 pKa = 3.95

Molecular weight: 53.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W1KLS9|A0A0W1KLS9_9ACTO Putative competence-damage inducible protein OS=Trueperella bernardiae OX=59561 GN=cinA PE=4 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.84RR5 pKa = 11.84TFQPNNRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84AKK16 pKa = 8.68THH18 pKa = 5.34GFRR21 pKa = 11.84KK22 pKa = 9.94RR23 pKa = 11.84MSTRR27 pKa = 11.84AGRR30 pKa = 11.84AVLAARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.74GRR41 pKa = 11.84AKK43 pKa = 10.68LSAA46 pKa = 3.92

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1762

0

1762

607078

29

3009

344.5

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.341 ± 0.089

0.63 ± 0.019

6.042 ± 0.049

6.472 ± 0.06

3.211 ± 0.032

8.71 ± 0.053

2.008 ± 0.027

5.063 ± 0.038

3.059 ± 0.044

9.484 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.022

2.589 ± 0.035

4.962 ± 0.038

3.028 ± 0.029

6.489 ± 0.067

5.504 ± 0.041

5.827 ± 0.045

8.513 ± 0.053

1.377 ± 0.026

2.376 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski