Grapevine Cabernet Sauvignon reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1YWF1|A0A0K1YWF1_9REOV Protein P4 (Fragment) OS=Grapevine Cabernet Sauvignon reovirus OX=1640277 PE=4 SV=1
TT1 pKa = 6.81SQDD4 pKa = 3.34FNSDD8 pKa = 3.28RR9 pKa = 11.84LRR11 pKa = 11.84QIYY14 pKa = 9.98LVEE17 pKa = 4.29PEE19 pKa = 4.39VNDD22 pKa = 3.23ARR24 pKa = 11.84AEE26 pKa = 4.08IPHH29 pKa = 5.85VAEE32 pKa = 4.55SDD34 pKa = 3.82QNDD37 pKa = 3.82EE38 pKa = 5.13DD39 pKa = 4.14YY40 pKa = 11.32QDD42 pKa = 4.09YY43 pKa = 11.19EE44 pKa = 6.17RR45 pKa = 11.84MDD47 pKa = 3.66QDD49 pKa = 3.76PQIQHH54 pKa = 6.9DD55 pKa = 5.27PIPMVADD62 pKa = 3.62AAIRR66 pKa = 11.84EE67 pKa = 4.31LVPARR72 pKa = 11.84MIEE75 pKa = 4.07NVNEE79 pKa = 3.95YY80 pKa = 10.57NEE82 pKa = 4.24NVSDD86 pKa = 4.26SGDD89 pKa = 3.84DD90 pKa = 3.76EE91 pKa = 4.72VDD93 pKa = 3.43EE94 pKa = 4.84MVDD97 pKa = 3.06NDD99 pKa = 3.81NVQEE103 pKa = 4.27GPIPQVEE110 pKa = 4.24DD111 pKa = 3.95QEE113 pKa = 4.68DD114 pKa = 3.53QPAYY118 pKa = 10.25VIPRR122 pKa = 11.84LSTVRR127 pKa = 11.84SVYY130 pKa = 10.39LEE132 pKa = 3.68RR133 pKa = 11.84LFARR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84VGEE142 pKa = 3.93DD143 pKa = 2.7QYY145 pKa = 11.56IQIRR149 pKa = 11.84PRR151 pKa = 11.84FAPDD155 pKa = 3.26VVPDD159 pKa = 4.35FATVMDD165 pKa = 4.58FNYY168 pKa = 8.97KK169 pKa = 8.72TVPQDD174 pKa = 2.5GWLNEE179 pKa = 4.09AGLEE183 pKa = 4.01LSLSGGLINGEE194 pKa = 4.61RR195 pKa = 11.84IPVSQFDD202 pKa = 3.91NLISLTIPQEE212 pKa = 4.02EE213 pKa = 4.29QTQTNYY219 pKa = 10.0VAPRR223 pKa = 11.84PLIFNVIPKK232 pKa = 9.89RR233 pKa = 11.84FSPVLLDD240 pKa = 4.29DD241 pKa = 4.17RR242 pKa = 11.84VNLEE246 pKa = 3.88GMLLEE251 pKa = 4.66LLQANVLPEE260 pKa = 4.13GLLDD264 pKa = 6.43RR265 pKa = 11.84IDD267 pKa = 3.94QVCGNYY273 pKa = 10.44LYY275 pKa = 9.77GTEE278 pKa = 4.03PQFLDD283 pKa = 3.91RR284 pKa = 11.84LTTPAQRR291 pKa = 11.84NSQTIVMTVSGLVCSCYY308 pKa = 9.2NTCPKK313 pKa = 10.3KK314 pKa = 10.41YY315 pKa = 9.33RR316 pKa = 11.84NSIVPIEE323 pKa = 4.45VGRR326 pKa = 11.84QLAVKK331 pKa = 9.75VRR333 pKa = 11.84QLIATIRR340 pKa = 11.84EE341 pKa = 4.15GTYY344 pKa = 10.82VDD346 pKa = 3.77VTTLFANWFRR356 pKa = 11.84LLYY359 pKa = 9.84PEE361 pKa = 5.81AYY363 pKa = 9.2MPLTNQLTNSFFHH376 pKa = 6.91WKK378 pKa = 9.99RR379 pKa = 11.84MPAYY383 pKa = 9.53EE384 pKa = 4.66DD385 pKa = 3.25FHH387 pKa = 8.24LVVPFLEE394 pKa = 4.78GAEE397 pKa = 4.12DD398 pKa = 3.67QQNRR402 pKa = 11.84PTDD405 pKa = 3.79LYY407 pKa = 11.13KK408 pKa = 10.81YY409 pKa = 6.0WTPTITPEE417 pKa = 4.38ILRR420 pKa = 11.84QSIRR424 pKa = 11.84TLPFFRR430 pKa = 11.84LLLDD434 pKa = 3.57VCSTDD439 pKa = 3.16ANLPFEE445 pKa = 4.37EE446 pKa = 4.63RR447 pKa = 11.84RR448 pKa = 11.84NTMSEE453 pKa = 3.68NMIVYY458 pKa = 9.49LKK460 pKa = 10.5IIFHH464 pKa = 6.69CLSLAYY470 pKa = 10.09VLYY473 pKa = 9.99WDD475 pKa = 4.62PCDD478 pKa = 3.36PSYY481 pKa = 11.58YY482 pKa = 10.06LIGMYY487 pKa = 9.8PPNDD491 pKa = 3.23IKK493 pKa = 11.41SSVTTKK499 pKa = 10.31IISKK503 pKa = 10.36ANPLTEE509 pKa = 3.96KK510 pKa = 11.05AFII513 pKa = 4.39

Molecular weight: 59.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1YWH9|A0A0K1YWH9_9REOV Structural protein P4 (Fragment) OS=Grapevine Cabernet Sauvignon reovirus OX=1640277 PE=4 SV=1
MM1 pKa = 7.74ANTLTIDD8 pKa = 3.44SLLPSLSATYY18 pKa = 10.3GATSQSPKK26 pKa = 10.49NPTNILVALSKK37 pKa = 10.54IFNSPSQGAPQRR49 pKa = 11.84IVQQGLGLTTCTSISLLVTLEE70 pKa = 3.98QVKK73 pKa = 9.8EE74 pKa = 3.95LHH76 pKa = 6.53EE77 pKa = 4.61LLLLYY82 pKa = 9.23TSRR85 pKa = 11.84WQDD88 pKa = 3.05DD89 pKa = 3.88RR90 pKa = 11.84LTGIVSLLQDD100 pKa = 4.01IINRR104 pKa = 11.84ARR106 pKa = 11.84KK107 pKa = 7.13TRR109 pKa = 11.84RR110 pKa = 11.84CYY112 pKa = 10.68LSPTFIARR120 pKa = 11.84LRR122 pKa = 11.84SVVRR126 pKa = 11.84VQLKK130 pKa = 9.99KK131 pKa = 10.3EE132 pKa = 3.81LRR134 pKa = 11.84AMISRR139 pKa = 11.84RR140 pKa = 11.84TIDD143 pKa = 3.71LFVMCSAPQEE153 pKa = 4.22FTFNPFQNRR162 pKa = 11.84IALTNEE168 pKa = 4.52SITKK172 pKa = 9.68IYY174 pKa = 10.67EE175 pKa = 4.03DD176 pKa = 3.3VTRR179 pKa = 11.84RR180 pKa = 11.84SVADD184 pKa = 3.38TAFQWFGMDD193 pKa = 4.76FGVTPYY199 pKa = 10.53RR200 pKa = 11.84YY201 pKa = 10.03GNHH204 pKa = 6.07VYY206 pKa = 10.52TNDD209 pKa = 3.44SLAMIEE215 pKa = 4.38PSSHH219 pKa = 6.19LLAMGQAIQNQAYY232 pKa = 10.34AEE234 pKa = 4.16IFAQSVPFSSPPQCVPXGEE253 pKa = 4.3FTLAACPMANRR264 pKa = 11.84VLKK267 pKa = 10.57DD268 pKa = 3.05SYY270 pKa = 10.04TWYY273 pKa = 10.83SKK275 pKa = 11.18AFNVQQRR282 pKa = 11.84ARR284 pKa = 11.84MIPNFWNEE292 pKa = 4.25DD293 pKa = 3.43LTDD296 pKa = 3.83VNWNSGGNTFGFPTWITDD314 pKa = 3.69STIKK318 pKa = 9.01WQYY321 pKa = 8.22TLYY324 pKa = 10.72FIKK327 pKa = 10.4DD328 pKa = 3.71YY329 pKa = 11.17INTFTALYY337 pKa = 10.22VMRR340 pKa = 11.84GG341 pKa = 3.29

Molecular weight: 38.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7907

128

1246

718.8

81.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.298 ± 0.227

1.72 ± 0.235

5.792 ± 0.32

6.071 ± 0.395

4.325 ± 0.137

5.249 ± 0.326

1.884 ± 0.14

6.488 ± 0.322

4.996 ± 0.351

9.346 ± 0.422

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.131

4.528 ± 0.23

5.109 ± 0.429

4.136 ± 0.33

5.514 ± 0.248

8.372 ± 0.487

6.071 ± 0.308

6.576 ± 0.24

1.277 ± 0.101

3.604 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski