Cellulomonas sp. KH9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3U2F3|A0A1I3U2F3_9CELL Allantoinase OS=Cellulomonas sp. KH9 OX=1855324 GN=SAMN05216467_0891 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84TTKK5 pKa = 10.44NSRR8 pKa = 11.84TAVLTTGAVAALLALTACAGSSSAAEE34 pKa = 4.12GAGEE38 pKa = 4.01VTYY41 pKa = 10.89PEE43 pKa = 4.73LADD46 pKa = 3.47SAACVEE52 pKa = 4.49LRR54 pKa = 11.84EE55 pKa = 4.16QNPDD59 pKa = 3.1LVGTTKK65 pKa = 10.46TNALNPHH72 pKa = 5.18TPGYY76 pKa = 10.45EE77 pKa = 4.02VVNPEE82 pKa = 4.25NPDD85 pKa = 2.99EE86 pKa = 5.02FMGFDD91 pKa = 3.99IDD93 pKa = 5.04LGDD96 pKa = 5.7AIGDD100 pKa = 3.86CLGFDD105 pKa = 3.58VDD107 pKa = 3.99YY108 pKa = 11.17LPVGFSEE115 pKa = 4.74LVPTVASGQADD126 pKa = 4.09WIISNLYY133 pKa = 8.08ATEE136 pKa = 3.95EE137 pKa = 4.12RR138 pKa = 11.84AHH140 pKa = 6.5GGVDD144 pKa = 4.48FISYY148 pKa = 10.73SKK150 pKa = 11.02VFDD153 pKa = 5.15GILVTTGNPQGITGIDD169 pKa = 3.24TSLCGTTVALNKK181 pKa = 10.51GYY183 pKa = 11.09VEE185 pKa = 4.43VPLVEE190 pKa = 4.44ALVPEE195 pKa = 4.78CEE197 pKa = 4.21AAGLEE202 pKa = 4.22APTVSLFDD210 pKa = 3.8SSADD214 pKa = 3.46CVQAILAGRR223 pKa = 11.84ADD225 pKa = 3.81AYY227 pKa = 10.18INDD230 pKa = 3.59VNTVRR235 pKa = 11.84GYY237 pKa = 9.86VAQHH241 pKa = 6.96PDD243 pKa = 3.61DD244 pKa = 5.9LDD246 pKa = 3.66TAEE249 pKa = 4.61TVMLDD254 pKa = 3.38YY255 pKa = 11.0EE256 pKa = 4.21IGIGVLQGDD265 pKa = 3.91HH266 pKa = 7.04AFRR269 pKa = 11.84DD270 pKa = 4.02AVQAALVEE278 pKa = 4.37IQDD281 pKa = 4.5SGLQDD286 pKa = 3.39EE287 pKa = 5.46LAGKK291 pKa = 9.43WSLDD295 pKa = 3.5EE296 pKa = 4.23NALAEE301 pKa = 4.18PTVLSVGG308 pKa = 3.6

Molecular weight: 32.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3U6C6|A0A1I3U6C6_9CELL Amino acid ABC transporter ATP-binding protein PAAT family OS=Cellulomonas sp. KH9 OX=1855324 GN=SAMN05216467_0988 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3696

0

3696

1259060

29

2701

340.7

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.711 ± 0.065

0.551 ± 0.009

6.681 ± 0.037

4.937 ± 0.034

2.346 ± 0.025

9.544 ± 0.04

2.144 ± 0.019

2.418 ± 0.029

1.169 ± 0.023

10.28 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.472 ± 0.015

1.355 ± 0.022

6.359 ± 0.035

2.656 ± 0.018

8.129 ± 0.049

4.513 ± 0.026

6.624 ± 0.044

10.747 ± 0.049

1.602 ± 0.018

1.763 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski