endosymbiont of Sipalinus gigas

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Nardonella

Average proteome isoelectric point is 8.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5T4H4|A0A2Z5T4H4_9GAMM 50S ribosomal protein L22 OS=endosymbiont of Sipalinus gigas OX=1972134 GN=rplV PE=3 SV=1
MM1 pKa = 7.34SGKK4 pKa = 10.42GINKK8 pKa = 9.54VILIGNLGQDD18 pKa = 3.25PEE20 pKa = 4.26IRR22 pKa = 11.84YY23 pKa = 8.06MPNGNAVTNIRR34 pKa = 11.84LATSEE39 pKa = 3.9NWKK42 pKa = 10.48DD43 pKa = 3.49KK44 pKa = 9.72QTGEE48 pKa = 4.26VKK50 pKa = 10.54EE51 pKa = 3.92KK52 pKa = 10.31TEE54 pKa = 3.22WHH56 pKa = 6.44RR57 pKa = 11.84IVFFGKK63 pKa = 9.98LAEE66 pKa = 4.28IANEE70 pKa = 3.88FLKK73 pKa = 10.7KK74 pKa = 10.49GSQIYY79 pKa = 10.14VEE81 pKa = 4.68GQLQTRR87 pKa = 11.84KK88 pKa = 8.8WQDD91 pKa = 2.92NNGNNKK97 pKa = 10.18YY98 pKa = 7.74ITEE101 pKa = 4.47VIVSIGGTMQMLGNKK116 pKa = 8.49NNKK119 pKa = 9.65NIEE122 pKa = 4.13NKK124 pKa = 10.26EE125 pKa = 3.99DD126 pKa = 4.23FLNEE130 pKa = 4.49DD131 pKa = 5.23NILLDD136 pKa = 3.6NKK138 pKa = 10.73KK139 pKa = 10.53DD140 pKa = 3.73FEE142 pKa = 5.43DD143 pKa = 4.41IKK145 pKa = 11.44DD146 pKa = 3.83DD147 pKa = 5.27DD148 pKa = 4.16ISSSLSEE155 pKa = 4.21FDD157 pKa = 5.63DD158 pKa = 4.79EE159 pKa = 5.64ISFF162 pKa = 4.41

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5T9J0|A0A2Z5T9J0_9GAMM 60 kDa chaperonin OS=endosymbiont of Sipalinus gigas OX=1972134 GN=groL PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.61RR14 pKa = 11.84KK15 pKa = 7.93IGFRR19 pKa = 11.84IRR21 pKa = 11.84MSTKK25 pKa = 9.88SGRR28 pKa = 11.84KK29 pKa = 8.52ILNNRR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.58KK38 pKa = 9.85RR39 pKa = 11.84NNLIVAA45 pKa = 4.68

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

70736

37

1353

310.2

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.723 ± 0.117

1.008 ± 0.046

4.61 ± 0.11

4.757 ± 0.155

5.46 ± 0.131

4.132 ± 0.149

1.031 ± 0.045

16.402 ± 0.247

12.904 ± 0.203

9.548 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.393 ± 0.057

12.746 ± 0.242

2.041 ± 0.075

1.193 ± 0.069

2.511 ± 0.094

6.697 ± 0.134

2.967 ± 0.087

3.085 ± 0.116

0.561 ± 0.053

5.231 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski