Mycobacterium phage Imvubu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L7G6|A0A6B9L7G6_9CAUD Uncharacterized protein OS=Mycobacterium phage Imvubu OX=2686233 GN=46 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84CGSGRR8 pKa = 11.84ISDD11 pKa = 3.71MMEE14 pKa = 4.45AMTQPDD20 pKa = 3.91PSDD23 pKa = 3.47PTFDD27 pKa = 3.59PAEE30 pKa = 4.17FEE32 pKa = 3.99WALGYY37 pKa = 10.68EE38 pKa = 4.44YY39 pKa = 10.59TSDD42 pKa = 3.96PLGLADD48 pKa = 4.42DD49 pKa = 4.39WEE51 pKa = 5.09FIYY54 pKa = 11.03QSMPYY59 pKa = 10.1EE60 pKa = 4.11LALEE64 pKa = 4.28NYY66 pKa = 8.34RR67 pKa = 11.84ATSSAIEE74 pKa = 4.08NTPGGIPNIRR84 pKa = 11.84NLGVYY89 pKa = 7.35FTPPVTWQPFDD100 pKa = 3.86VPPAPAAEE108 pKa = 4.33VVADD112 pKa = 4.61EE113 pKa = 5.31RR114 pKa = 11.84PADD117 pKa = 3.8TPNQQ121 pKa = 3.56

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9L7P5|A0A6B9L7P5_9CAUD Uncharacterized protein OS=Mycobacterium phage Imvubu OX=2686233 GN=55 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84EE3 pKa = 3.8STRR6 pKa = 11.84QARR9 pKa = 11.84AGAFLGPARR18 pKa = 11.84LRR20 pKa = 11.84PVKK23 pKa = 8.75ITKK26 pKa = 8.34YY27 pKa = 8.11TLCDD31 pKa = 3.44TPGCNHH37 pKa = 6.84DD38 pKa = 3.86RR39 pKa = 11.84AAHH42 pKa = 5.72PRR44 pKa = 11.84GEE46 pKa = 4.48GCRR49 pKa = 11.84HH50 pKa = 5.7NRR52 pKa = 11.84GQGPFSCPCEE62 pKa = 4.05QFTVRR67 pKa = 11.84LPDD70 pKa = 3.46VPKK73 pKa = 10.32RR74 pKa = 11.84PAPRR78 pKa = 11.84VYY80 pKa = 10.28TPEE83 pKa = 5.15DD84 pKa = 3.34VAAWRR89 pKa = 11.84ADD91 pKa = 3.49LEE93 pKa = 4.57RR94 pKa = 11.84GLSYY98 pKa = 11.01RR99 pKa = 11.84EE100 pKa = 3.31VHH102 pKa = 6.06RR103 pKa = 11.84RR104 pKa = 11.84HH105 pKa = 6.01KK106 pKa = 10.8VSEE109 pKa = 3.85HH110 pKa = 4.72TLRR113 pKa = 11.84SKK115 pKa = 11.22LPGYY119 pKa = 9.7NGNSTHH125 pKa = 7.2PNGTNGIRR133 pKa = 11.84NRR135 pKa = 11.84TT136 pKa = 3.56

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

21950

31

2127

215.2

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.665 ± 0.416

1.093 ± 0.157

6.569 ± 0.307

5.476 ± 0.251

2.378 ± 0.13

9.718 ± 0.49

1.941 ± 0.184

4.055 ± 0.161

2.374 ± 0.146

7.85 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.103

2.852 ± 0.213

6.674 ± 0.219

3.171 ± 0.154

7.39 ± 0.418

4.907 ± 0.199

7.052 ± 0.19

7.526 ± 0.255

1.927 ± 0.146

2.114 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski