Bacillus marisflavi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Rossellomorea

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0G0X4|A0A0M0G0X4_9BACI Lysine 6-monooxygenase OS=Bacillus marisflavi OX=189381 GN=AF331_20080 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 6.54LINHH6 pKa = 7.05FIEE9 pKa = 4.98GLHH12 pKa = 5.47EE13 pKa = 4.44HH14 pKa = 7.25LLPEE18 pKa = 4.48EE19 pKa = 4.39LEE21 pKa = 4.32EE22 pKa = 4.1LQKK25 pKa = 11.16SHH27 pKa = 6.38GASPEE32 pKa = 3.85DD33 pKa = 3.72LEE35 pKa = 4.33EE36 pKa = 4.43LRR38 pKa = 11.84RR39 pKa = 11.84LYY41 pKa = 10.04PDD43 pKa = 3.85CPEE46 pKa = 4.46SLLSLLKK53 pKa = 11.06NIDD56 pKa = 3.32GTFWRR61 pKa = 11.84QYY63 pKa = 9.98GEE65 pKa = 4.0EE66 pKa = 4.29KK67 pKa = 10.39IAVCILGSDD76 pKa = 4.14VEE78 pKa = 4.56DD79 pKa = 3.45GRR81 pKa = 11.84YY82 pKa = 9.11PYY84 pKa = 10.99YY85 pKa = 10.88LLSTQQMIDD94 pKa = 3.18SSKK97 pKa = 10.88EE98 pKa = 3.97EE99 pKa = 3.83VDD101 pKa = 3.12TSYY104 pKa = 11.35LSEE107 pKa = 4.26YY108 pKa = 10.82EE109 pKa = 3.97EE110 pKa = 6.02DD111 pKa = 4.69DD112 pKa = 3.2VDD114 pKa = 5.29SRR116 pKa = 11.84INQDD120 pKa = 2.61GLYY123 pKa = 8.89PGTYY127 pKa = 9.15IHH129 pKa = 7.28FSDD132 pKa = 4.63CMNNGGTSSLFIDD145 pKa = 5.56FNPTDD150 pKa = 3.55QGVSGQIVCFLHH162 pKa = 7.4DD163 pKa = 4.27PDD165 pKa = 4.06EE166 pKa = 4.94FKK168 pKa = 11.28VIADD172 pKa = 3.7SFDD175 pKa = 4.14EE176 pKa = 4.31YY177 pKa = 11.55VQGLIDD183 pKa = 3.76GGFVFLEE190 pKa = 4.28EE191 pKa = 4.28EE192 pKa = 4.27EE193 pKa = 4.31

Molecular weight: 22.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J5V4S8|A0A0J5V4S8_9BACI Acyl carrier protein OS=Bacillus marisflavi OX=189381 GN=acpP PE=3 SV=1
MM1 pKa = 7.38AQVQYY6 pKa = 10.93YY7 pKa = 7.69GTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 10.2SSVARR18 pKa = 11.84VRR20 pKa = 11.84LVPGNGNIIINDD32 pKa = 3.91RR33 pKa = 11.84EE34 pKa = 4.22VEE36 pKa = 4.35VYY38 pKa = 10.39IPFAALRR45 pKa = 11.84EE46 pKa = 4.43VIKK49 pKa = 10.8QPLVATEE56 pKa = 3.92TLGNYY61 pKa = 9.24DD62 pKa = 3.21VLVNVHH68 pKa = 6.13GGGYY72 pKa = 8.19TGQAGAIRR80 pKa = 11.84HH81 pKa = 6.03GIARR85 pKa = 11.84ALLQVDD91 pKa = 3.74PEE93 pKa = 4.35YY94 pKa = 10.87RR95 pKa = 11.84GSLKK99 pKa = 10.54RR100 pKa = 11.84AGLLTRR106 pKa = 11.84DD107 pKa = 3.24SRR109 pKa = 11.84MKK111 pKa = 10.09EE112 pKa = 3.6RR113 pKa = 11.84KK114 pKa = 9.59KK115 pKa = 11.04YY116 pKa = 8.89GLKK119 pKa = 9.63GARR122 pKa = 11.84RR123 pKa = 11.84APQFSKK129 pKa = 10.95RR130 pKa = 3.57

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4143

0

4143

1204750

26

2702

290.8

32.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.1 ± 0.04

0.674 ± 0.011

5.52 ± 0.031

7.699 ± 0.048

4.458 ± 0.032

7.577 ± 0.033

2.272 ± 0.023

7.226 ± 0.037

6.309 ± 0.034

9.839 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.947 ± 0.021

3.534 ± 0.024

3.796 ± 0.02

3.348 ± 0.024

4.513 ± 0.034

6.21 ± 0.03

5.383 ± 0.032

7.184 ± 0.036

1.077 ± 0.016

3.335 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski