Enterococcus phage LY0323

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PZU0|A0A2Z4PZU0_9CAUD Uncharacterized protein OS=Enterococcus phage LY0323 OX=2233540 PE=4 SV=1
MM1 pKa = 6.97TVKK4 pKa = 9.88TNYY7 pKa = 9.87YY8 pKa = 9.42ICLTNNEE15 pKa = 4.58LFTLYY20 pKa = 10.13TEE22 pKa = 4.3EE23 pKa = 5.74PILVMYY29 pKa = 9.04EE30 pKa = 3.86KK31 pKa = 10.61AVEE34 pKa = 3.98NDD36 pKa = 3.07EE37 pKa = 5.09KK38 pKa = 11.13LLKK41 pKa = 10.46LEE43 pKa = 4.21KK44 pKa = 10.36PEE46 pKa = 4.73AIEE49 pKa = 5.19IDD51 pKa = 4.12GEE53 pKa = 4.31MQDD56 pKa = 3.57TFITIPLDD64 pKa = 3.76SILYY68 pKa = 8.73VLEE71 pKa = 4.49DD72 pKa = 3.8AKK74 pKa = 11.25CC75 pKa = 3.22

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4PZB7|A0A2Z4PZB7_9CAUD Holin OS=Enterococcus phage LY0323 OX=2233540 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 4.74EE3 pKa = 3.59WRR5 pKa = 11.84EE6 pKa = 3.55VDD8 pKa = 3.82NLNKK12 pKa = 10.54YY13 pKa = 10.0KK14 pKa = 10.38ISNLGRR20 pKa = 11.84IKK22 pKa = 10.67SKK24 pKa = 10.95ARR26 pKa = 11.84IVRR29 pKa = 11.84CNTGVLEE36 pKa = 4.26TKK38 pKa = 10.38EE39 pKa = 4.59IILKK43 pKa = 9.51NQTRR47 pKa = 11.84GKK49 pKa = 10.46RR50 pKa = 11.84GFEE53 pKa = 4.22YY54 pKa = 10.93NCVRR58 pKa = 11.84LTDD61 pKa = 3.56NKK63 pKa = 10.81GYY65 pKa = 8.03TKK67 pKa = 9.88TYY69 pKa = 9.32SVHH72 pKa = 6.71RR73 pKa = 11.84LVAEE77 pKa = 3.98AFIPNPEE84 pKa = 4.12NKK86 pKa = 8.62PTVDD90 pKa = 3.45HH91 pKa = 6.89INRR94 pKa = 11.84DD95 pKa = 3.42RR96 pKa = 11.84LDD98 pKa = 3.41NRR100 pKa = 11.84VEE102 pKa = 3.87NLRR105 pKa = 11.84WATYY109 pKa = 10.11IEE111 pKa = 3.92QQLNKK116 pKa = 10.09KK117 pKa = 8.56ATGLKK122 pKa = 10.18KK123 pKa = 10.34KK124 pKa = 9.97IKK126 pKa = 10.67SIDD129 pKa = 3.3KK130 pKa = 10.13EE131 pKa = 4.44GNEE134 pKa = 4.53TYY136 pKa = 10.62YY137 pKa = 11.28SSIRR141 pKa = 11.84EE142 pKa = 3.95ASNALNIDD150 pKa = 3.46EE151 pKa = 4.36GTISKK156 pKa = 10.32VISDD160 pKa = 3.62KK161 pKa = 11.26YY162 pKa = 9.74INKK165 pKa = 8.63TAGGFKK171 pKa = 10.44FEE173 pKa = 4.83SIEE176 pKa = 3.96NN177 pKa = 3.76

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12399

47

1410

206.7

23.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.775 ± 0.405

0.669 ± 0.142

6.017 ± 0.258

8.138 ± 0.378

4.105 ± 0.22

6.315 ± 0.532

1.54 ± 0.186

6.831 ± 0.225

8.92 ± 0.412

8.162 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.173

6.622 ± 0.255

2.871 ± 0.201

3.71 ± 0.145

3.605 ± 0.255

5.517 ± 0.227

6.251 ± 0.392

6.791 ± 0.244

1.266 ± 0.1

4.089 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski