Mycobacterium phage Qyrzula

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Rosebushvirus; Mycobacterium virus Godines

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19Z54|Q19Z54_9CAUD Uncharacterized protein OS=Mycobacterium phage Qyrzula OX=373414 GN=32 PE=4 SV=1
MM1 pKa = 8.02PEE3 pKa = 3.76NDD5 pKa = 3.18QNTWAVRR12 pKa = 11.84WEE14 pKa = 3.93AAQLPVGLIPEE25 pKa = 4.82CPPPPVYY32 pKa = 9.91PADD35 pKa = 3.46QLPPVTYY42 pKa = 10.52DD43 pKa = 3.38PATGNPIPPEE53 pKa = 3.95YY54 pKa = 10.31TPEE57 pKa = 3.94QQEE60 pKa = 4.82LVDD63 pKa = 4.31NYY65 pKa = 9.81QAQAEE70 pKa = 4.74VYY72 pKa = 9.54ADD74 pKa = 5.94CVAQWNAAVDD84 pKa = 3.69AVLADD89 pKa = 3.85DD90 pKa = 5.07ANWTIAMTTASSEE103 pKa = 4.4SVARR107 pKa = 11.84ALLAEE112 pKa = 4.65LRR114 pKa = 11.84LAHH117 pKa = 7.08AGDD120 pKa = 3.62KK121 pKa = 8.38YY122 pKa = 9.58TRR124 pKa = 11.84NFRR127 pKa = 11.84LVTAPPRR134 pKa = 11.84SWTVVEE140 pKa = 4.3

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19Z22|Q19Z22_9CAUD Uncharacterized protein OS=Mycobacterium phage Qyrzula OX=373414 GN=64 PE=4 SV=1
MM1 pKa = 7.77ANRR4 pKa = 11.84GAPMSKK10 pKa = 9.91GQRR13 pKa = 11.84PADD16 pKa = 3.47KK17 pKa = 9.39QTRR20 pKa = 11.84KK21 pKa = 9.95LKK23 pKa = 10.44RR24 pKa = 11.84QLEE27 pKa = 4.33RR28 pKa = 11.84AGLRR32 pKa = 11.84VTLGGNGHH40 pKa = 6.14WKK42 pKa = 10.2VYY44 pKa = 10.46DD45 pKa = 3.48GEE47 pKa = 4.84RR48 pKa = 11.84YY49 pKa = 10.08LLTFAGTSADD59 pKa = 3.5NMQSRR64 pKa = 11.84HH65 pKa = 4.43VRR67 pKa = 11.84RR68 pKa = 11.84DD69 pKa = 2.92LRR71 pKa = 11.84RR72 pKa = 11.84YY73 pKa = 9.62HH74 pKa = 6.81GIEE77 pKa = 4.6LEE79 pKa = 4.29EE80 pKa = 4.05QQ81 pKa = 3.19

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

20597

43

1882

254.3

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.084 ± 0.381

0.961 ± 0.121

6.229 ± 0.211

5.792 ± 0.266

2.617 ± 0.113

9.03 ± 0.546

2.078 ± 0.179

3.661 ± 0.155

2.767 ± 0.188

8.516 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.126

2.884 ± 0.133

6.006 ± 0.206

3.496 ± 0.148

6.671 ± 0.274

5.049 ± 0.166

6.87 ± 0.228

7.933 ± 0.228

1.966 ± 0.146

2.224 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski