Fistulifera solaris (Oleaginous diatom)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Bacillariophyceae; Bacillariophycidae; Naviculales; Naviculaceae; Fistulifera

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z5KJQ0|A0A1Z5KJQ0_FISSO Uncharacterized protein OS=Fistulifera solaris OX=1519565 GN=FisN_16Lh186 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 9.1ITTFATSLLLASVLVPNQASQVDD25 pKa = 3.71EE26 pKa = 4.48ALPHH30 pKa = 5.48FRR32 pKa = 11.84ARR34 pKa = 11.84SLLTAVEE41 pKa = 4.5LGNGAQLSVNINAPATGSSVVVDD64 pKa = 4.17DD65 pKa = 4.19TCAASTPFDD74 pKa = 3.45GSASVGKK81 pKa = 10.36GNPDD85 pKa = 2.69VTYY88 pKa = 10.65IFIIDD93 pKa = 3.81EE94 pKa = 4.45SGSTSGIIAGIKK106 pKa = 10.44AFFEE110 pKa = 4.63DD111 pKa = 3.25LTDD114 pKa = 3.41IVFSEE119 pKa = 5.16GSALNAGVVRR129 pKa = 11.84FGSSAIRR136 pKa = 11.84MSALTDD142 pKa = 4.51DD143 pKa = 3.39IAAIKK148 pKa = 10.4DD149 pKa = 3.83VIDD152 pKa = 3.64NPFLNGPSTNCADD165 pKa = 3.97SLVKK169 pKa = 10.52ARR171 pKa = 11.84EE172 pKa = 3.88LAEE175 pKa = 4.06TSTAATTIVLFAGDD189 pKa = 4.5GACNSGGSFVGPANDD204 pKa = 4.24LAGLANTNVIVEE216 pKa = 4.77TIAIGIGCQGDD227 pKa = 3.91LGSIPRR233 pKa = 11.84NGGSCKK239 pKa = 10.42AVTSVNDD246 pKa = 3.3FDD248 pKa = 3.59ITTVIGTTLKK258 pKa = 10.85DD259 pKa = 3.12VAYY262 pKa = 9.85NLNAGAFASLTTSPSGDD279 pKa = 3.29TAGPATKK286 pKa = 9.75TFVQAIPINLGPNDD300 pKa = 4.37LCVKK304 pKa = 9.77ATGNDD309 pKa = 3.09EE310 pKa = 4.78VGQQDD315 pKa = 3.65VDD317 pKa = 3.81VLEE320 pKa = 5.36CISIVGVDD328 pKa = 3.85ASAPTITCPANIITATDD345 pKa = 3.21AGLCEE350 pKa = 4.48SSVATGSATAIDD362 pKa = 3.98NCSDD366 pKa = 3.47GLSPQASATGPFPLGISTVTFSVVDD391 pKa = 3.65ASGNPASCDD400 pKa = 3.33MSVEE404 pKa = 4.29VYY406 pKa = 10.62DD407 pKa = 3.95GEE409 pKa = 4.53EE410 pKa = 4.52PILLCPADD418 pKa = 3.72MTVSTDD424 pKa = 3.86AGICTATIEE433 pKa = 4.38IPVATVTDD441 pKa = 3.66NCDD444 pKa = 3.07QGLTPSTTAVNPFALGSTTVEE465 pKa = 3.92YY466 pKa = 9.95TVADD470 pKa = 3.6NAGNTDD476 pKa = 2.94ACTFAVVVEE485 pKa = 4.61DD486 pKa = 4.39NEE488 pKa = 4.63APTATCSPANNPGGNVPKK506 pKa = 10.72ANNQDD511 pKa = 3.27GFFVLGGEE519 pKa = 4.62DD520 pKa = 3.36NCSVLSYY527 pKa = 10.58QVVDD531 pKa = 3.93SVSGFVFGPFPSGTVFKK548 pKa = 9.86YY549 pKa = 9.47TEE551 pKa = 4.58APGSTPSQKK560 pKa = 10.39PGTGNVNYY568 pKa = 9.75FLKK571 pKa = 10.89GKK573 pKa = 10.03GDD575 pKa = 3.76AYY577 pKa = 11.63VLVTDD582 pKa = 4.9GAGNTAMAPCLVPPKK597 pKa = 9.86PSRR600 pKa = 11.84LLRR603 pKa = 11.84GII605 pKa = 4.15

Molecular weight: 60.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z5KD27|A0A1Z5KD27_FISSO Isoform of A0A1Z5KCW6 Uncharacterized protein (Fragment) OS=Fistulifera solaris OX=1519565 GN=FisN_26Lh116 PE=4 SV=1
RR1 pKa = 7.04RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SS14 pKa = 3.02

Molecular weight: 2.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20304

15

20319

10061793

14

7171

495.2

55.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.031 ± 0.014

1.622 ± 0.008

6.036 ± 0.013

6.699 ± 0.018

3.845 ± 0.011

5.942 ± 0.015

2.406 ± 0.009

4.721 ± 0.008

5.108 ± 0.016

9.451 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.006

3.894 ± 0.008

5.081 ± 0.017

4.423 ± 0.014

5.764 ± 0.012

8.275 ± 0.021

5.83 ± 0.012

6.493 ± 0.011

1.318 ± 0.006

2.657 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski