Medicago sativa amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2V0A0B8|A0A2V0A0B8_9VIRU ORF1+2p OS=Medicago sativa amalgavirus 1 OX=2069326 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 4.38FEE5 pKa = 4.39AQRR8 pKa = 11.84DD9 pKa = 3.92DD10 pKa = 5.06NIAANAPIGGLSSKK24 pKa = 8.01QQEE27 pKa = 4.29IDD29 pKa = 3.4TVTAAITPLLAAGFPQAIFNYY50 pKa = 10.32DD51 pKa = 3.5DD52 pKa = 4.24LLLRR56 pKa = 11.84GYY58 pKa = 7.12TAKK61 pKa = 10.03TFCDD65 pKa = 5.23FIKK68 pKa = 10.16PLSAITEE75 pKa = 4.04RR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.35LISLCALGNNRR89 pKa = 11.84FWDD92 pKa = 3.6MSVVAEE98 pKa = 4.57LDD100 pKa = 3.35EE101 pKa = 4.34FLNFVKK107 pKa = 10.02WLKK110 pKa = 10.79SPEE113 pKa = 3.9GRR115 pKa = 11.84DD116 pKa = 3.51AQTQAAKK123 pKa = 10.32KK124 pKa = 9.67RR125 pKa = 11.84ALNKK129 pKa = 9.82KK130 pKa = 9.92ASDD133 pKa = 3.5GMSTKK138 pKa = 10.57DD139 pKa = 3.32VALVQMGNAIIADD152 pKa = 3.74YY153 pKa = 10.58QRR155 pKa = 11.84EE156 pKa = 4.18RR157 pKa = 11.84KK158 pKa = 8.4QRR160 pKa = 11.84RR161 pKa = 11.84FPIEE165 pKa = 3.64EE166 pKa = 3.69EE167 pKa = 3.84MAEE170 pKa = 4.25LRR172 pKa = 11.84RR173 pKa = 11.84QLRR176 pKa = 11.84QLDD179 pKa = 4.0EE180 pKa = 4.07EE181 pKa = 4.34LQAVEE186 pKa = 4.6EE187 pKa = 4.45EE188 pKa = 4.43IKK190 pKa = 11.01VKK192 pKa = 10.49YY193 pKa = 10.57GPVALYY199 pKa = 8.86EE200 pKa = 4.64GPDD203 pKa = 3.4NTRR206 pKa = 11.84VKK208 pKa = 10.78SDD210 pKa = 3.59AYY212 pKa = 11.43LMYY215 pKa = 10.52QDD217 pKa = 4.71DD218 pKa = 4.42CRR220 pKa = 11.84KK221 pKa = 9.74KK222 pKa = 9.78GYY224 pKa = 9.7RR225 pKa = 11.84AIAQYY230 pKa = 10.71QGGFEE235 pKa = 4.14KK236 pKa = 10.62AVEE239 pKa = 3.94LFGNKK244 pKa = 7.29VRR246 pKa = 11.84EE247 pKa = 4.21AHH249 pKa = 5.71FCAYY253 pKa = 10.48LSDD256 pKa = 4.01PARR259 pKa = 11.84SDD261 pKa = 3.67FVRR264 pKa = 11.84DD265 pKa = 3.53YY266 pKa = 9.79YY267 pKa = 10.07NQKK270 pKa = 9.46IVHH273 pKa = 6.41LEE275 pKa = 3.55RR276 pKa = 11.84SGEE279 pKa = 4.08KK280 pKa = 9.92KK281 pKa = 9.73QAGSFRR287 pKa = 11.84SLLVAAHH294 pKa = 6.63GEE296 pKa = 4.24VAVNVPIVNPFNFDD310 pKa = 3.56GEE312 pKa = 4.4DD313 pKa = 3.37SGRR316 pKa = 11.84EE317 pKa = 3.86SSQTGGNANQPPATKK332 pKa = 10.0RR333 pKa = 11.84RR334 pKa = 11.84RR335 pKa = 11.84QKK337 pKa = 10.73SRR339 pKa = 11.84AEE341 pKa = 3.92RR342 pKa = 11.84EE343 pKa = 4.2AEE345 pKa = 3.9RR346 pKa = 11.84LQIDD350 pKa = 3.77QPVRR354 pKa = 11.84TQARR358 pKa = 11.84TSRR361 pKa = 11.84VHH363 pKa = 5.9NKK365 pKa = 9.3SRR367 pKa = 11.84NAGGQDD373 pKa = 2.87QDD375 pKa = 3.82LQIVGVGAGEE385 pKa = 4.81DD386 pKa = 3.72DD387 pKa = 3.76PHH389 pKa = 7.84VQEE392 pKa = 4.79QVV394 pKa = 2.81

Molecular weight:
44.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2V0A0B8|A0A2V0A0B8_9VIRU ORF1+2p OS=Medicago sativa amalgavirus 1 OX=2069326 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 4.38FEE5 pKa = 4.39AQRR8 pKa = 11.84DD9 pKa = 3.92DD10 pKa = 5.06NIAANAPIGGLSSKK24 pKa = 8.01QQEE27 pKa = 4.29IDD29 pKa = 3.4TVTAAITPLLAAGFPQAIFNYY50 pKa = 10.32DD51 pKa = 3.5DD52 pKa = 4.24LLLRR56 pKa = 11.84GYY58 pKa = 7.12TAKK61 pKa = 10.03TFCDD65 pKa = 5.23FIKK68 pKa = 10.16PLSAITEE75 pKa = 4.04RR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.35LISLCALGNNRR89 pKa = 11.84FWDD92 pKa = 3.6MSVVAEE98 pKa = 4.57LDD100 pKa = 3.35EE101 pKa = 4.34FLNFVKK107 pKa = 10.02WLKK110 pKa = 10.79SPEE113 pKa = 3.9GRR115 pKa = 11.84DD116 pKa = 3.51AQTQAAKK123 pKa = 10.32KK124 pKa = 9.67RR125 pKa = 11.84ALNKK129 pKa = 9.82KK130 pKa = 9.92ASDD133 pKa = 3.5GMSTKK138 pKa = 10.57DD139 pKa = 3.32VALVQMGNAIIADD152 pKa = 3.74YY153 pKa = 10.58QRR155 pKa = 11.84EE156 pKa = 4.18RR157 pKa = 11.84KK158 pKa = 8.4QRR160 pKa = 11.84RR161 pKa = 11.84FPIEE165 pKa = 3.64EE166 pKa = 3.69EE167 pKa = 3.84MAEE170 pKa = 4.25LRR172 pKa = 11.84RR173 pKa = 11.84QLRR176 pKa = 11.84QLDD179 pKa = 4.0EE180 pKa = 4.07EE181 pKa = 4.34LQAVEE186 pKa = 4.6EE187 pKa = 4.45EE188 pKa = 4.43IKK190 pKa = 11.01VKK192 pKa = 10.49YY193 pKa = 10.57GPVALYY199 pKa = 8.86EE200 pKa = 4.64GPDD203 pKa = 3.4NTRR206 pKa = 11.84VKK208 pKa = 10.78SDD210 pKa = 3.59AYY212 pKa = 11.43LMYY215 pKa = 10.52QDD217 pKa = 4.71DD218 pKa = 4.42CRR220 pKa = 11.84KK221 pKa = 9.74KK222 pKa = 9.78GYY224 pKa = 9.7RR225 pKa = 11.84AIAQYY230 pKa = 10.71QGGFEE235 pKa = 4.14KK236 pKa = 10.62AVEE239 pKa = 3.94LFGNKK244 pKa = 7.29VRR246 pKa = 11.84EE247 pKa = 4.21AHH249 pKa = 5.71FCAYY253 pKa = 10.48LSDD256 pKa = 4.01PARR259 pKa = 11.84SDD261 pKa = 3.67FVRR264 pKa = 11.84DD265 pKa = 3.53YY266 pKa = 9.79YY267 pKa = 10.07NQKK270 pKa = 9.46IVHH273 pKa = 6.41LEE275 pKa = 3.55RR276 pKa = 11.84SGEE279 pKa = 4.08KK280 pKa = 9.98KK281 pKa = 9.91QAGSFAVSWWPLMEE295 pKa = 4.54KK296 pKa = 9.44WLLMFPLSTRR306 pKa = 11.84STLMEE311 pKa = 5.3KK312 pKa = 10.09IPVGKK317 pKa = 8.72VHH319 pKa = 7.07KK320 pKa = 10.31PGEE323 pKa = 4.46TPTNRR328 pKa = 11.84PLLRR332 pKa = 11.84GADD335 pKa = 3.42KK336 pKa = 11.25SRR338 pKa = 11.84VLNVRR343 pKa = 11.84QSGYY347 pKa = 10.79KK348 pKa = 10.01SISRR352 pKa = 11.84LEE354 pKa = 4.25RR355 pKa = 11.84KK356 pKa = 9.69HH357 pKa = 6.23EE358 pKa = 3.98LQGCITSQGMPEE370 pKa = 3.72GRR372 pKa = 11.84IKK374 pKa = 10.4IFKK377 pKa = 9.66SWEE380 pKa = 4.05SEE382 pKa = 4.0PGRR385 pKa = 11.84MIPMSRR391 pKa = 11.84SKK393 pKa = 11.12YY394 pKa = 7.64EE395 pKa = 3.86CAVRR399 pKa = 11.84WVIGGGEE406 pKa = 3.96VRR408 pKa = 11.84NWKK411 pKa = 9.66VDD413 pKa = 2.82SSMYY417 pKa = 10.35RR418 pKa = 11.84GGGSSNDD425 pKa = 3.5ALKK428 pKa = 11.08LLANASTVRR437 pKa = 11.84PGKK440 pKa = 10.0LLRR443 pKa = 11.84DD444 pKa = 3.67VYY446 pKa = 10.47SFRR449 pKa = 11.84VARR452 pKa = 11.84ARR454 pKa = 11.84LQLPGDD460 pKa = 4.01FSVPDD465 pKa = 4.08GPDD468 pKa = 2.69ACRR471 pKa = 11.84VKK473 pKa = 10.94NFNNFATSGPVLKK486 pKa = 10.94AFGVRR491 pKa = 11.84SKK493 pKa = 11.42NGLRR497 pKa = 11.84QLLQDD502 pKa = 3.67EE503 pKa = 4.49AWWYY507 pKa = 10.52FNSFGNSDD515 pKa = 3.93FGVEE519 pKa = 3.94GLPWFGARR527 pKa = 11.84LGFRR531 pKa = 11.84SKK533 pKa = 10.97LVTEE537 pKa = 4.58EE538 pKa = 3.97KK539 pKa = 10.41ARR541 pKa = 11.84KK542 pKa = 9.2KK543 pKa = 10.28ISEE546 pKa = 4.15GDD548 pKa = 3.75SVGRR552 pKa = 11.84AVMMMDD558 pKa = 5.17ALEE561 pKa = 4.23QCCSSPLYY569 pKa = 10.51NVLSTYY575 pKa = 8.45TFHH578 pKa = 7.56KK579 pKa = 10.07RR580 pKa = 11.84LNRR583 pKa = 11.84RR584 pKa = 11.84SGFKK588 pKa = 10.34NAVVKK593 pKa = 10.89ASSDD597 pKa = 3.05WAHH600 pKa = 6.24IWNGVKK606 pKa = 10.23DD607 pKa = 3.51AAVIVEE613 pKa = 4.4LDD615 pKa = 2.89WSKK618 pKa = 10.98FDD620 pKa = 3.95RR621 pKa = 11.84EE622 pKa = 4.19RR623 pKa = 11.84PRR625 pKa = 11.84EE626 pKa = 3.97DD627 pKa = 3.66LEE629 pKa = 4.65FMVSLISSCFNPKK642 pKa = 9.27SARR645 pKa = 11.84EE646 pKa = 3.8EE647 pKa = 4.22RR648 pKa = 11.84LLHH651 pKa = 7.2AYY653 pKa = 5.92TTSNFRR659 pKa = 11.84ALVEE663 pKa = 4.16RR664 pKa = 11.84PVFLDD669 pKa = 3.17GGGVFGIEE677 pKa = 3.97GMVPSGSLWTGWLDD691 pKa = 3.31TALNILYY698 pKa = 8.53MKK700 pKa = 10.05AVCAEE705 pKa = 4.06IGVGDD710 pKa = 4.56DD711 pKa = 4.32DD712 pKa = 5.55VEE714 pKa = 4.64VMCAGDD720 pKa = 4.8DD721 pKa = 3.67NLTLFKK727 pKa = 10.59FDD729 pKa = 4.01PGEE732 pKa = 4.06ANLRR736 pKa = 11.84RR737 pKa = 11.84IRR739 pKa = 11.84DD740 pKa = 3.74LLNDD744 pKa = 3.7WFLAGISEE752 pKa = 4.74DD753 pKa = 3.12EE754 pKa = 4.4FLIHH758 pKa = 6.97RR759 pKa = 11.84PPYY762 pKa = 10.28HH763 pKa = 4.66VTKK766 pKa = 10.7VQACFPEE773 pKa = 4.8GTDD776 pKa = 3.77LSRR779 pKa = 11.84GTSGMLKK786 pKa = 9.64KK787 pKa = 10.59AVWVPFEE794 pKa = 4.32GEE796 pKa = 4.29VVIDD800 pKa = 3.58NARR803 pKa = 11.84GRR805 pKa = 11.84SHH807 pKa = 6.44RR808 pKa = 11.84WEE810 pKa = 4.06YY811 pKa = 10.99RR812 pKa = 11.84FSGCPKK818 pKa = 10.15FLSCYY823 pKa = 8.11WLHH826 pKa = 7.32DD827 pKa = 3.8GLPIRR832 pKa = 11.84PAHH835 pKa = 6.99DD836 pKa = 3.77NLEE839 pKa = 4.15KK840 pKa = 10.97LLWPEE845 pKa = 4.36GLHH848 pKa = 7.51DD849 pKa = 6.67DD850 pKa = 3.4IDD852 pKa = 4.38VYY854 pKa = 10.95EE855 pKa = 4.41GAVISMVVDD864 pKa = 3.69NPHH867 pKa = 5.86NHH869 pKa = 6.37HH870 pKa = 6.75NCNHH874 pKa = 5.61MLSRR878 pKa = 11.84FIIIRR883 pKa = 11.84EE884 pKa = 3.93ARR886 pKa = 11.84RR887 pKa = 11.84LGASVEE893 pKa = 4.39DD894 pKa = 3.7PFIPIKK900 pKa = 10.37HH901 pKa = 5.49GSIRR905 pKa = 11.84PVGDD909 pKa = 3.26EE910 pKa = 3.72PVPYY914 pKa = 10.02PEE916 pKa = 4.13LAPWRR921 pKa = 11.84RR922 pKa = 11.84APGGYY927 pKa = 9.84KK928 pKa = 10.4LEE930 pKa = 5.09DD931 pKa = 3.48YY932 pKa = 10.68PEE934 pKa = 4.29NAEE937 pKa = 4.42HH938 pKa = 6.15IQVFRR943 pKa = 11.84DD944 pKa = 3.97FMQGVSSLYY953 pKa = 10.32LRR955 pKa = 11.84EE956 pKa = 4.05ATGGVDD962 pKa = 2.83AWQFMDD968 pKa = 5.25IIRR971 pKa = 11.84GDD973 pKa = 3.53AFVGEE978 pKa = 4.47GQFGNDD984 pKa = 3.17LRR986 pKa = 11.84SWLNWLHH993 pKa = 5.94HH994 pKa = 6.34HH995 pKa = 7.41PISKK999 pKa = 10.09YY1000 pKa = 10.17LRR1002 pKa = 11.84EE1003 pKa = 4.18TKK1005 pKa = 9.23TFRR1008 pKa = 11.84LKK1010 pKa = 10.98DD1011 pKa = 3.52GADD1014 pKa = 3.3PDD1016 pKa = 4.0APKK1019 pKa = 10.72DD1020 pKa = 3.63LEE1022 pKa = 4.2KK1023 pKa = 10.86AGRR1026 pKa = 11.84ALRR1029 pKa = 11.84ALRR1032 pKa = 11.84GRR1034 pKa = 11.84LEE1036 pKa = 4.34LGGFACVEE1044 pKa = 4.13DD1045 pKa = 4.49FVRR1048 pKa = 11.84WLDD1051 pKa = 4.05GIRR1054 pKa = 11.84SSRR1057 pKa = 11.84EE1058 pKa = 3.58

Molecular weight:
120.01 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1452

394

1058

726.0

82.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.609 ± 1.223

1.446 ± 0.229

6.749 ± 0.19

7.163 ± 0.374

4.683 ± 0.33

7.576 ± 0.518

1.86 ± 0.313

4.339 ± 0.013

5.854 ± 0.126

8.54 ± 0.76

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.325

4.063 ± 0.268

4.545 ± 0.392

3.926 ± 1.687

8.196 ± 0.096

6.129 ± 0.559

2.961 ± 0.179

6.405 ± 0.032

1.928 ± 0.754

2.893 ± 0.081

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski