Mycobacterium conspicuum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1T608|A0A1X1T608_9MYCO Transglutaminase OS=Mycobacterium conspicuum OX=44010 GN=AWC00_17595 PE=4 SV=1
MM1 pKa = 7.36SFVIAASDD9 pKa = 3.3AMAAATTDD17 pKa = 3.81LADD20 pKa = 4.3LGSTLRR26 pKa = 11.84SAHH29 pKa = 6.07AVAAPATTGLVAAGADD45 pKa = 3.58EE46 pKa = 4.49VSAAVATLFGSHH58 pKa = 5.68AQAYY62 pKa = 7.36QAISAHH68 pKa = 5.57AAEE71 pKa = 4.29FHH73 pKa = 6.74SRR75 pKa = 11.84FVQLLSAGAGQYY87 pKa = 11.68ALTEE91 pKa = 4.15ATNASPLQAVEE102 pKa = 4.57HH103 pKa = 6.29GLLNAINAPTQALVGRR119 pKa = 11.84PLIGNGADD127 pKa = 3.46GVAGGTLAQANGGAGGILFGNGGAGATDD155 pKa = 3.41AAGQGGSGGAAGLIGNGGAGGAGATGGAGGRR186 pKa = 11.84GGAGGLLYY194 pKa = 11.04GNGGAGGSGGDD205 pKa = 3.94STDD208 pKa = 3.34GAAGGAGGAGGSAGFFGNGGAGGSGGAGGDD238 pKa = 3.35ATVAGGNGGAGGNGGAGGNAGHH260 pKa = 6.49VLGDD264 pKa = 4.03GGAGGAGGAGGGGGAVTGGTVISGDD289 pKa = 2.97IGGAGGNGGAGGPTFVGQGGNGGAGGAGGAAGAVTDD325 pKa = 3.73STIYY329 pKa = 10.95SGDD332 pKa = 3.28TGGAGGNGGAGNLAGLFGNGGHH354 pKa = 6.75GGAGGNGGAAGTVTNSTVQTGDD376 pKa = 2.77TGGAGGAGGAASLGGFVGNGGNGGAGGAGGDD407 pKa = 3.57AGAVTGGSNIATGDD421 pKa = 3.41NGGAGGDD428 pKa = 3.44GGAGNIAAYY437 pKa = 10.08VGSGGNGGAGGNGGNAGAVSASSTVDD463 pKa = 3.01NGDD466 pKa = 3.32NGGDD470 pKa = 3.7GGDD473 pKa = 3.87GGTGTGGASSFGGTASHH490 pKa = 6.95GGNGGAGGAAGSVTDD505 pKa = 4.05SAVHH509 pKa = 6.3SGDD512 pKa = 3.3NGGAGGDD519 pKa = 3.59GGTIVAGKK527 pKa = 9.78GGNGGNGGNGGDD539 pKa = 3.86GGALTGSTVTSGDD552 pKa = 3.24NGGTGGAGGAGGMIGTSGNGGNGGDD577 pKa = 4.2GGDD580 pKa = 3.84GGAVSASSAVTVGDD594 pKa = 3.4NGGAGGAGGAGSSSGYY610 pKa = 9.1FANGGNGGAGGNGGAGGAVSGSSTVSTGDD639 pKa = 3.01NGGAGGAGGAGAIAGFIGNGGNGGAGGDD667 pKa = 3.68GGGGGGG673 pKa = 3.88

Molecular weight: 55.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1T242|A0A1X1T242_9MYCO Alanine and proline-rich secreted protein Apa OS=Mycobacterium conspicuum OX=44010 GN=AWC00_19620 PE=3 SV=1
MM1 pKa = 7.61PGITRR6 pKa = 11.84SSAHH10 pKa = 5.54STSSIAEE17 pKa = 4.1RR18 pKa = 11.84LTLRR22 pKa = 11.84PDD24 pKa = 3.4TTRR27 pKa = 11.84TASRR31 pKa = 11.84NTDD34 pKa = 2.81GAADD38 pKa = 3.86AGRR41 pKa = 11.84PRR43 pKa = 11.84VPEE46 pKa = 4.09AGKK49 pKa = 10.19AAGRR53 pKa = 11.84EE54 pKa = 4.28SGSGRR59 pKa = 11.84SDD61 pKa = 2.95GGSGVDD67 pKa = 3.72RR68 pKa = 11.84VPAGTNGLVICAKK81 pKa = 9.42SVRR84 pKa = 11.84DD85 pKa = 3.77TVDD88 pKa = 4.03GIVDD92 pKa = 3.93AEE94 pKa = 4.4LSASDD99 pKa = 3.81ALGLRR104 pKa = 11.84FPRR107 pKa = 11.84PNMVASAAALFEE119 pKa = 4.39PAEE122 pKa = 4.22PWAHH126 pKa = 5.62GRR128 pKa = 11.84DD129 pKa = 3.66RR130 pKa = 11.84LDD132 pKa = 3.37VEE134 pKa = 4.61PEE136 pKa = 3.81CGRR139 pKa = 11.84FRR141 pKa = 11.84ITIGPGVVRR150 pKa = 11.84LGWTNPVRR158 pKa = 11.84AEE160 pKa = 4.02KK161 pKa = 10.56ASEE164 pKa = 4.09RR165 pKa = 11.84TVGQHH170 pKa = 4.8MQDD173 pKa = 2.94VAGEE177 pKa = 3.99VDD179 pKa = 4.09RR180 pKa = 11.84IKK182 pKa = 10.86AGRR185 pKa = 11.84DD186 pKa = 3.27VPDD189 pKa = 3.89PSGRR193 pKa = 11.84AITEE197 pKa = 3.83WSRR200 pKa = 11.84KK201 pKa = 8.11SRR203 pKa = 11.84AAMCRR208 pKa = 11.84TFAEE212 pKa = 4.0LDD214 pKa = 3.51YY215 pKa = 11.07TPMVEE220 pKa = 4.21SGRR223 pKa = 11.84VPAMVTLTYY232 pKa = 10.39PGDD235 pKa = 3.94WEE237 pKa = 4.76SVAPDD242 pKa = 3.41GASVKK247 pKa = 10.04RR248 pKa = 11.84HH249 pKa = 3.72MMLWRR254 pKa = 11.84KK255 pKa = 9.49RR256 pKa = 11.84FQRR259 pKa = 11.84EE260 pKa = 3.24WGEE263 pKa = 3.53PARR266 pKa = 11.84YY267 pKa = 7.08IWKK270 pKa = 8.8MEE272 pKa = 3.95FQRR275 pKa = 11.84RR276 pKa = 11.84GAPHH280 pKa = 5.72IHH282 pKa = 6.48LWMAPPYY289 pKa = 8.9TVGRR293 pKa = 11.84SGRR296 pKa = 11.84RR297 pKa = 11.84FSEE300 pKa = 3.94WLSQEE305 pKa = 3.45WAEE308 pKa = 4.63VVAHH312 pKa = 7.05PDD314 pKa = 3.09PEE316 pKa = 3.96QRR318 pKa = 11.84TRR320 pKa = 11.84HH321 pKa = 5.3LRR323 pKa = 11.84AGTAIDD329 pKa = 3.61SLNGLRR335 pKa = 11.84ACDD338 pKa = 3.92PKK340 pKa = 10.89RR341 pKa = 11.84LAIYY345 pKa = 7.5FTKK348 pKa = 10.43HH349 pKa = 5.28SSPNRR354 pKa = 11.84LGDD357 pKa = 3.4KK358 pKa = 9.68EE359 pKa = 4.21YY360 pKa = 10.91QHH362 pKa = 6.79IVPEE366 pKa = 4.14AWRR369 pKa = 11.84RR370 pKa = 11.84PGRR373 pKa = 11.84GPGRR377 pKa = 11.84FWGVHH382 pKa = 3.94GLEE385 pKa = 4.08RR386 pKa = 11.84ATAVVEE392 pKa = 4.31IAQDD396 pKa = 3.18AYY398 pKa = 9.93LTARR402 pKa = 11.84RR403 pKa = 11.84IIRR406 pKa = 11.84RR407 pKa = 11.84WSRR410 pKa = 11.84SQAIHH415 pKa = 6.74SDD417 pKa = 3.57CTHH420 pKa = 7.07CFPTALVPRR429 pKa = 11.84MTRR432 pKa = 11.84VAVQRR437 pKa = 11.84TNSKK441 pKa = 7.97TGKK444 pKa = 8.26VRR446 pKa = 11.84HH447 pKa = 6.12RR448 pKa = 11.84NVCRR452 pKa = 11.84RR453 pKa = 11.84RR454 pKa = 11.84LLCRR458 pKa = 11.84QGGFAGGYY466 pKa = 10.28ALVNDD471 pKa = 5.09GPAFASQLAAALSADD486 pKa = 3.75RR487 pKa = 11.84AKK489 pKa = 10.73AVRR492 pKa = 11.84DD493 pKa = 3.44EE494 pKa = 4.02QLAGRR499 pKa = 11.84KK500 pKa = 8.49

Molecular weight: 55.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5457

0

5457

1826921

42

10008

334.8

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.137 ± 0.049

0.834 ± 0.01

6.189 ± 0.027

5.1 ± 0.036

3.081 ± 0.019

9.442 ± 0.145

2.256 ± 0.015

4.241 ± 0.023

2.195 ± 0.022

9.738 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.015

2.438 ± 0.032

5.831 ± 0.033

3.082 ± 0.015

7.166 ± 0.041

5.369 ± 0.023

5.828 ± 0.026

8.381 ± 0.035

1.496 ± 0.013

2.167 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski