Rice latent virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0WZF4|A0A2D0WZF4_9GEMI Replication-associated protein OS=Rice latent virus 2 OX=2012857 PE=3 SV=1
MM1 pKa = 7.63TNTEE5 pKa = 4.18SASEE9 pKa = 4.06QPHH12 pKa = 6.03NFAFQNKK19 pKa = 7.79NVFLTYY25 pKa = 9.47PRR27 pKa = 11.84CSQEE31 pKa = 3.66PHH33 pKa = 5.46TMGLFLWNLLRR44 pKa = 11.84PYY46 pKa = 10.81GVLCASEE53 pKa = 4.11LHH55 pKa = 6.94KK56 pKa = 11.21DD57 pKa = 3.9GTPHH61 pKa = 6.9LHH63 pKa = 6.99ALAQTRR69 pKa = 11.84HH70 pKa = 6.97RR71 pKa = 11.84IRR73 pKa = 11.84THH75 pKa = 6.75DD76 pKa = 3.52SSFFDD81 pKa = 4.11NMDD84 pKa = 3.44YY85 pKa = 10.92HH86 pKa = 7.07PNIQGTRR93 pKa = 11.84NPKK96 pKa = 10.15AVLAYY101 pKa = 9.52ILKK104 pKa = 9.97HH105 pKa = 5.53PTGEE109 pKa = 3.62WHH111 pKa = 6.77KK112 pKa = 11.24GSFRR116 pKa = 11.84PRR118 pKa = 11.84GSFGVPPTGNGEE130 pKa = 4.32AGPSTTIRR138 pKa = 11.84RR139 pKa = 11.84AAGDD143 pKa = 3.64SSSSAEE149 pKa = 4.04GGNTSSEE156 pKa = 4.2LANKK160 pKa = 9.02RR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84MTKK166 pKa = 9.73DD167 pKa = 2.84QIMADD172 pKa = 3.59ILRR175 pKa = 11.84SATSRR180 pKa = 11.84MEE182 pKa = 3.79YY183 pKa = 9.9MNGVKK188 pKa = 9.68KK189 pKa = 9.99WFPYY193 pKa = 9.99DD194 pKa = 3.23YY195 pKa = 10.39CARR198 pKa = 11.84LQAWEE203 pKa = 4.19YY204 pKa = 11.12ASQKK208 pKa = 10.26LFPDD212 pKa = 3.79PPVTYY217 pKa = 9.67EE218 pKa = 3.92PPFNDD223 pKa = 4.38SMFHH227 pKa = 5.2CHH229 pKa = 5.44EE230 pKa = 4.78TLTEE234 pKa = 3.83WAAEE238 pKa = 4.21NIYY241 pKa = 10.68HH242 pKa = 5.66VTPQVYY248 pKa = 10.36SLLHH252 pKa = 5.19PHH254 pKa = 6.63SDD256 pKa = 3.47AEE258 pKa = 4.5ADD260 pKa = 4.22LRR262 pKa = 11.84WLHH265 pKa = 6.0DD266 pKa = 3.09TCFASRR272 pKa = 11.84RR273 pKa = 11.84DD274 pKa = 3.71QSASTSADD282 pKa = 3.16HH283 pKa = 6.88QGQEE287 pKa = 4.15SLLGLEE293 pKa = 4.9ASADD297 pKa = 3.99TITTRR302 pKa = 11.84ITT304 pKa = 3.03

Molecular weight: 34.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0WZE5|A0A2D0WZE5_9GEMI Movement protein OS=Rice latent virus 2 OX=2012857 PE=3 SV=1
MM1 pKa = 7.63PARR4 pKa = 11.84TGSARR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 10.35RR12 pKa = 11.84KK13 pKa = 9.87DD14 pKa = 2.87DD15 pKa = 3.82WAWDD19 pKa = 3.32SATRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84VRR27 pKa = 11.84PRR29 pKa = 11.84TSYY32 pKa = 10.63VPRR35 pKa = 11.84GGPRR39 pKa = 11.84VRR41 pKa = 11.84RR42 pKa = 11.84PSLQYY47 pKa = 11.11VNPGLLGSTAVHH59 pKa = 5.78VADD62 pKa = 3.92TNGEE66 pKa = 3.93VRR68 pKa = 11.84IITGYY73 pKa = 10.68SRR75 pKa = 11.84GSDD78 pKa = 3.21EE79 pKa = 4.66QSRR82 pKa = 11.84HH83 pKa = 4.17TNEE86 pKa = 3.98TITYY90 pKa = 9.21KK91 pKa = 10.82LSLDD95 pKa = 3.79LYY97 pKa = 8.16VTVDD101 pKa = 3.7TEE103 pKa = 4.02CQKK106 pKa = 11.1YY107 pKa = 8.98VGKK110 pKa = 10.43GVAVAWLVYY119 pKa = 9.79DD120 pKa = 4.19AQPTGEE126 pKa = 4.46MPAASTIFPHH136 pKa = 5.72VADD139 pKa = 4.57MATAPCTWKK148 pKa = 10.46VGRR151 pKa = 11.84EE152 pKa = 3.84VCHH155 pKa = 6.21RR156 pKa = 11.84FVVKK160 pKa = 10.3RR161 pKa = 11.84RR162 pKa = 11.84WVITLEE168 pKa = 4.21TNGRR172 pKa = 11.84VAGTVFQGANGVPPCNRR189 pKa = 11.84TVYY192 pKa = 9.79FHH194 pKa = 7.13KK195 pKa = 9.97FCKK198 pKa = 10.03RR199 pKa = 11.84LGVRR203 pKa = 11.84TEE205 pKa = 3.77WKK207 pKa = 7.92NTSGGSIGDD216 pKa = 3.7IKK218 pKa = 10.2TGAPVHH224 pKa = 5.44SVRR227 pKa = 11.84GRR229 pKa = 11.84EE230 pKa = 3.75PDD232 pKa = 2.99

Molecular weight: 25.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1016

98

382

254.0

28.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.874 ± 0.367

2.264 ± 0.289

4.823 ± 0.417

4.626 ± 0.424

4.035 ± 0.592

7.283 ± 1.04

3.74 ± 0.748

3.642 ± 0.555

4.331 ± 0.488

6.398 ± 0.935

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.348

4.429 ± 0.816

6.693 ± 0.285

3.445 ± 0.53

7.185 ± 1.221

7.48 ± 0.692

7.874 ± 0.604

5.315 ± 2.027

2.264 ± 0.178

3.839 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski