Arthrobacter phage Bumble

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3VBW2|A0A7G3VBW2_9CAUD Uncharacterized protein OS=Arthrobacter phage Bumble OX=2743904 GN=51 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.55LTVHH6 pKa = 7.09LAHH9 pKa = 7.3PSGDD13 pKa = 3.51VTLEE17 pKa = 3.87SPEE20 pKa = 3.87IDD22 pKa = 5.17DD23 pKa = 3.6RR24 pKa = 11.84TEE26 pKa = 4.11DD27 pKa = 3.62YY28 pKa = 9.33EE29 pKa = 4.61ARR31 pKa = 11.84AILAAMYY38 pKa = 10.32FDD40 pKa = 3.98VLGEE44 pKa = 4.04RR45 pKa = 11.84LLGLLRR51 pKa = 11.84LARR54 pKa = 11.84SEE56 pKa = 4.39PDD58 pKa = 3.63LDD60 pKa = 4.08PDD62 pKa = 3.86YY63 pKa = 11.56APAPAEE69 pKa = 4.36AEE71 pKa = 4.36GADD74 pKa = 3.97PAPAVDD80 pKa = 4.89PEE82 pKa = 4.82LLPEE86 pKa = 4.4PAPAPGDD93 pKa = 3.76TVDD96 pKa = 5.5PEE98 pKa = 4.26LADD101 pKa = 3.61YY102 pKa = 10.6LAANPRR108 pKa = 3.52

Molecular weight: 11.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3VAC0|A0A7G3VAC0_9CAUD RecT-like ssDNA binding protein OS=Arthrobacter phage Bumble OX=2743904 GN=35 PE=4 SV=1
MM1 pKa = 6.96LTVVFDD7 pKa = 4.1PRR9 pKa = 11.84SNLAAIPPLTLRR21 pKa = 11.84GAYY24 pKa = 9.22EE25 pKa = 3.93EE26 pKa = 4.44EE27 pKa = 4.34VVVRR31 pKa = 11.84HH32 pKa = 5.14VHH34 pKa = 4.33THH36 pKa = 6.63LKK38 pKa = 10.01PKK40 pKa = 9.9DD41 pKa = 3.62RR42 pKa = 11.84KK43 pKa = 10.04DD44 pKa = 3.47FSLARR49 pKa = 11.84VPGSRR54 pKa = 11.84GGVVVLRR61 pKa = 11.84RR62 pKa = 11.84GKK64 pKa = 8.76EE65 pKa = 3.71QISFRR70 pKa = 11.84IEE72 pKa = 3.57GTARR76 pKa = 11.84TPDD79 pKa = 3.29GHH81 pKa = 6.81PPRR84 pKa = 11.84DD85 pKa = 3.42PRR87 pKa = 11.84AIVPPSWGAGSS98 pKa = 3.35

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12559

49

869

220.3

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.648 ± 0.445

0.358 ± 0.08

6.028 ± 0.293

6.386 ± 0.436

2.333 ± 0.188

8.95 ± 0.44

1.481 ± 0.188

4.029 ± 0.267

3.169 ± 0.239

8.958 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.585 ± 0.158

2.572 ± 0.187

6.585 ± 0.363

2.198 ± 0.148

7.341 ± 0.408

6.37 ± 0.411

6.426 ± 0.353

7.532 ± 0.358

1.441 ± 0.164

2.612 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski