Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira interrogans; Leptospira interrogans serovar Lai

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8F876|Q8F876_LEPIN Uncharacterized protein OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=LA_0682 PE=4 SV=1
MM1 pKa = 7.42NIEE4 pKa = 4.13IGKK7 pKa = 9.07GLNGIYY13 pKa = 10.06FGMSIDD19 pKa = 3.79EE20 pKa = 4.51VKK22 pKa = 10.93SKK24 pKa = 10.83LGEE27 pKa = 3.98PDD29 pKa = 3.6EE30 pKa = 5.53IYY32 pKa = 10.61DD33 pKa = 3.63YY34 pKa = 10.78DD35 pKa = 4.33YY36 pKa = 11.56EE37 pKa = 4.72GTQSTGYY44 pKa = 10.06EE45 pKa = 4.19YY46 pKa = 10.65FSEE49 pKa = 4.18EE50 pKa = 3.85SEE52 pKa = 4.6YY53 pKa = 11.05EE54 pKa = 3.74FDD56 pKa = 4.5QEE58 pKa = 4.51EE59 pKa = 4.31DD60 pKa = 3.25NKK62 pKa = 10.87LYY64 pKa = 10.84SITTSNPSIQLFGKK78 pKa = 9.23PIIGEE83 pKa = 4.16SIEE86 pKa = 4.4TIRR89 pKa = 11.84EE90 pKa = 3.99LLNQNGIDD98 pKa = 4.27DD99 pKa = 4.65LEE101 pKa = 5.19EE102 pKa = 6.1DD103 pKa = 4.9DD104 pKa = 5.91EE105 pKa = 4.68EE106 pKa = 4.46EE107 pKa = 4.12HH108 pKa = 7.22DD109 pKa = 3.95QEE111 pKa = 4.61EE112 pKa = 4.57DD113 pKa = 3.31HH114 pKa = 7.3DD115 pKa = 4.44HH116 pKa = 6.3TGVTAFSDD124 pKa = 3.63KK125 pKa = 10.7INAVFQFEE133 pKa = 4.04EE134 pKa = 4.57DD135 pKa = 3.46EE136 pKa = 4.45LVFFGFSPLFKK147 pKa = 9.98DD148 pKa = 5.78DD149 pKa = 3.9QIDD152 pKa = 3.61WPKK155 pKa = 10.27YY156 pKa = 8.83

Molecular weight: 18.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8F1N8|Q8F1N8_LEPIN Uncharacterized protein OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=LA_3092 PE=4 SV=1
MM1 pKa = 7.66WVRR4 pKa = 11.84FLNRR8 pKa = 11.84SAKK11 pKa = 9.0PRR13 pKa = 11.84LVRR16 pKa = 11.84VPTFLNRR23 pKa = 11.84SVKK26 pKa = 9.44PRR28 pKa = 11.84LVRR31 pKa = 11.84VPTFLNRR38 pKa = 11.84SVKK41 pKa = 9.44PRR43 pKa = 11.84LVRR46 pKa = 11.84VPTFF50 pKa = 3.17

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3676

0

3676

1159747

37

3088

315.5

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.238 ± 0.034

0.88 ± 0.014

4.731 ± 0.029

7.146 ± 0.049

5.734 ± 0.05

6.462 ± 0.043

1.692 ± 0.017

8.077 ± 0.04

7.819 ± 0.041

10.386 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.017

5.068 ± 0.034

3.876 ± 0.024

3.298 ± 0.024

4.417 ± 0.029

7.841 ± 0.041

5.055 ± 0.039

5.675 ± 0.032

1.128 ± 0.016

3.577 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski