Amino acid dipepetide frequency for Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.664AlaAla: 10.664 ± 0.178
1.324AlaCys: 1.324 ± 0.046
5.007AlaAsp: 5.007 ± 0.088
5.716AlaGlu: 5.716 ± 0.102
3.601AlaPhe: 3.601 ± 0.108
9.119AlaGly: 9.119 ± 0.133
1.628AlaHis: 1.628 ± 0.043
6.749AlaIle: 6.749 ± 0.119
4.082AlaLys: 4.082 ± 0.093
9.636AlaLeu: 9.636 ± 0.236
2.438AlaMet: 2.438 ± 0.058
2.73AlaAsn: 2.73 ± 0.07
3.66AlaPro: 3.66 ± 0.083
2.724AlaGln: 2.724 ± 0.074
5.014AlaArg: 5.014 ± 0.099
4.674AlaSer: 4.674 ± 0.082
5.202AlaThr: 5.202 ± 0.106
7.332AlaVal: 7.332 ± 0.115
0.917AlaTrp: 0.917 ± 0.045
2.42AlaTyr: 2.42 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
1.159CysAla: 1.159 ± 0.039
0.258CysCys: 0.258 ± 0.022
0.664CysAsp: 0.664 ± 0.033
0.668CysGlu: 0.668 ± 0.032
0.43CysPhe: 0.43 ± 0.027
1.593CysGly: 1.593 ± 0.057
0.32CysHis: 0.32 ± 0.02
0.927CysIle: 0.927 ± 0.041
0.379CysLys: 0.379 ± 0.025
0.98CysLeu: 0.98 ± 0.047
0.356CysMet: 0.356 ± 0.023
0.4CysAsn: 0.4 ± 0.025
0.913CysPro: 0.913 ± 0.048
0.329CysGln: 0.329 ± 0.021
0.973CysArg: 0.973 ± 0.041
0.85CysSer: 0.85 ± 0.041
1.081CysThr: 1.081 ± 0.044
0.852CysVal: 0.852 ± 0.038
0.129CysTrp: 0.129 ± 0.013
0.342CysTyr: 0.342 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
5.154AspAla: 5.154 ± 0.114
0.646AspCys: 0.646 ± 0.031
2.303AspAsp: 2.303 ± 0.062
3.524AspGlu: 3.524 ± 0.082
1.965AspPhe: 1.965 ± 0.062
3.691AspGly: 3.691 ± 0.079
1.2AspHis: 1.2 ± 0.045
3.987AspIle: 3.987 ± 0.086
2.013AspLys: 2.013 ± 0.064
5.637AspLeu: 5.637 ± 0.095
1.25AspMet: 1.25 ± 0.047
1.683AspAsn: 1.683 ± 0.056
3.458AspPro: 3.458 ± 0.078
1.45AspGln: 1.45 ± 0.044
3.445AspArg: 3.445 ± 0.075
2.45AspSer: 2.45 ± 0.059
3.177AspThr: 3.177 ± 0.067
3.653AspVal: 3.653 ± 0.086
0.547AspTrp: 0.547 ± 0.025
1.603AspTyr: 1.603 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
4.916GluAla: 4.916 ± 0.141
0.767GluCys: 0.767 ± 0.032
2.684GluAsp: 2.684 ± 0.076
4.398GluGlu: 4.398 ± 0.098
2.101GluPhe: 2.101 ± 0.057
3.962GluGly: 3.962 ± 0.079
1.316GluHis: 1.316 ± 0.047
5.38GluIle: 5.38 ± 0.107
4.479GluLys: 4.479 ± 0.09
5.085GluLeu: 5.085 ± 0.096
1.666GluMet: 1.666 ± 0.051
2.505GluAsn: 2.505 ± 0.059
2.374GluPro: 2.374 ± 0.063
2.215GluGln: 2.215 ± 0.055
3.865GluArg: 3.865 ± 0.081
3.165GluSer: 3.165 ± 0.072
3.36GluThr: 3.36 ± 0.075
3.935GluVal: 3.935 ± 0.085
0.506GluTrp: 0.506 ± 0.024
1.93GluTyr: 1.93 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.672PheAla: 3.672 ± 0.079
0.668PheCys: 0.668 ± 0.029
2.278PheAsp: 2.278 ± 0.072
2.18PheGlu: 2.18 ± 0.066
1.963PhePhe: 1.963 ± 0.07
3.235PheGly: 3.235 ± 0.075
0.704PheHis: 0.704 ± 0.03
2.355PheIle: 2.355 ± 0.06
1.133PheLys: 1.133 ± 0.045
3.826PheLeu: 3.826 ± 0.091
0.782PheMet: 0.782 ± 0.034
1.226PheAsn: 1.226 ± 0.061
1.758PhePro: 1.758 ± 0.046
0.819PheGln: 0.819 ± 0.034
1.967PheArg: 1.967 ± 0.058
3.209PheSer: 3.209 ± 0.075
2.56PheThr: 2.56 ± 0.067
3.238PheVal: 3.238 ± 0.069
0.47PheTrp: 0.47 ± 0.029
1.12PheTyr: 1.12 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
6.617GlyAla: 6.617 ± 0.121
1.252GlyCys: 1.252 ± 0.048
3.958GlyAsp: 3.958 ± 0.084
4.511GlyGlu: 4.511 ± 0.088
3.158GlyPhe: 3.158 ± 0.066
5.828GlyGly: 5.828 ± 0.119
1.483GlyHis: 1.483 ± 0.049
7.215GlyIle: 7.215 ± 0.12
4.777GlyLys: 4.777 ± 0.104
6.442GlyLeu: 6.442 ± 0.105
2.355GlyMet: 2.355 ± 0.062
2.912GlyAsn: 2.912 ± 0.093
2.64GlyPro: 2.64 ± 0.066
2.134GlyGln: 2.134 ± 0.057
4.141GlyArg: 4.141 ± 0.091
5.228GlySer: 5.228 ± 0.103
5.817GlyThr: 5.817 ± 0.098
5.488GlyVal: 5.488 ± 0.102
0.947GlyTrp: 0.947 ± 0.034
2.887GlyTyr: 2.887 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.921HisAla: 1.921 ± 0.049
0.329HisCys: 0.329 ± 0.024
1.136HisAsp: 1.136 ± 0.034
1.197HisGlu: 1.197 ± 0.038
0.756HisPhe: 0.756 ± 0.036
1.548HisGly: 1.548 ± 0.054
0.579HisHis: 0.579 ± 0.03
1.351HisIle: 1.351 ± 0.051
0.751HisLys: 0.751 ± 0.032
1.856HisLeu: 1.856 ± 0.054
0.437HisMet: 0.437 ± 0.024
0.628HisAsn: 0.628 ± 0.028
1.368HisPro: 1.368 ± 0.052
0.565HisGln: 0.565 ± 0.027
1.228HisArg: 1.228 ± 0.044
1.041HisSer: 1.041 ± 0.037
1.145HisThr: 1.145 ± 0.038
1.354HisVal: 1.354 ± 0.048
0.198HisTrp: 0.198 ± 0.017
0.58HisTyr: 0.58 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
7.463IleAla: 7.463 ± 0.107
0.942IleCys: 0.942 ± 0.035
3.951IleAsp: 3.951 ± 0.075
4.069IleGlu: 4.069 ± 0.097
2.84IlePhe: 2.84 ± 0.071
5.556IleGly: 5.556 ± 0.104
1.485IleHis: 1.485 ± 0.043
4.694IleIle: 4.694 ± 0.1
2.629IleLys: 2.629 ± 0.062
6.907IleLeu: 6.907 ± 0.129
1.362IleMet: 1.362 ± 0.044
2.099IleAsn: 2.099 ± 0.06
4.041IlePro: 4.041 ± 0.082
1.866IleGln: 1.866 ± 0.056
4.672IleArg: 4.672 ± 0.104
4.696IleSer: 4.696 ± 0.09
4.768IleThr: 4.768 ± 0.103
5.53IleVal: 5.53 ± 0.102
0.616IleTrp: 0.616 ± 0.03
1.717IleTyr: 1.717 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
3.772LysAla: 3.772 ± 0.087
0.536LysCys: 0.536 ± 0.026
2.571LysAsp: 2.571 ± 0.068
3.79LysGlu: 3.79 ± 0.084
1.401LysPhe: 1.401 ± 0.045
3.498LysGly: 3.498 ± 0.093
0.741LysHis: 0.741 ± 0.031
3.61LysIle: 3.61 ± 0.082
4.519LysLys: 4.519 ± 0.102
3.078LysLeu: 3.078 ± 0.072
1.313LysMet: 1.313 ± 0.044
2.447LysAsn: 2.447 ± 0.067
1.967LysPro: 1.967 ± 0.054
1.309LysGln: 1.309 ± 0.046
2.457LysArg: 2.457 ± 0.072
2.564LysSer: 2.564 ± 0.062
2.964LysThr: 2.964 ± 0.074
3.029LysVal: 3.029 ± 0.075
0.378LysTrp: 0.378 ± 0.021
1.483LysTyr: 1.483 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
9.797LeuAla: 9.797 ± 0.166
1.257LeuCys: 1.257 ± 0.045
4.984LeuAsp: 4.984 ± 0.094
5.11LeuGlu: 5.11 ± 0.103
4.111LeuPhe: 4.111 ± 0.1
6.698LeuGly: 6.698 ± 0.107
1.769LeuHis: 1.769 ± 0.045
6.22LeuIle: 6.22 ± 0.127
4.135LeuLys: 4.135 ± 0.089
8.971LeuLeu: 8.971 ± 0.146
2.123LeuMet: 2.123 ± 0.053
2.899LeuAsn: 2.899 ± 0.067
4.636LeuPro: 4.636 ± 0.092
2.33LeuGln: 2.33 ± 0.061
4.699LeuArg: 4.699 ± 0.103
6.431LeuSer: 6.431 ± 0.106
5.154LeuThr: 5.154 ± 0.097
7.287LeuVal: 7.287 ± 0.121
0.83LeuTrp: 0.83 ± 0.038
2.49LeuTyr: 2.49 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.341MetAla: 2.341 ± 0.056
0.253MetCys: 0.253 ± 0.019
1.404MetAsp: 1.404 ± 0.047
1.441MetGlu: 1.441 ± 0.046
0.734MetPhe: 0.734 ± 0.034
1.915MetGly: 1.915 ± 0.052
0.511MetHis: 0.511 ± 0.028
1.749MetIle: 1.749 ± 0.051
1.475MetLys: 1.475 ± 0.051
2.091MetLeu: 2.091 ± 0.059
0.683MetMet: 0.683 ± 0.032
0.898MetAsn: 0.898 ± 0.038
1.32MetPro: 1.32 ± 0.04
0.894MetGln: 0.894 ± 0.033
1.217MetArg: 1.217 ± 0.042
1.328MetSer: 1.328 ± 0.043
1.692MetThr: 1.692 ± 0.058
1.763MetVal: 1.763 ± 0.047
0.139MetTrp: 0.139 ± 0.014
0.642MetTyr: 0.642 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.133AsnAla: 3.133 ± 0.077
0.407AsnCys: 0.407 ± 0.026
1.637AsnAsp: 1.637 ± 0.05
1.819AsnGlu: 1.819 ± 0.052
1.211AsnPhe: 1.211 ± 0.041
2.836AsnGly: 2.836 ± 0.087
0.588AsnHis: 0.588 ± 0.029
2.295AsnIle: 2.295 ± 0.068
1.175AsnLys: 1.175 ± 0.045
3.15AsnLeu: 3.15 ± 0.074
0.745AsnMet: 0.745 ± 0.032
1.195AsnAsn: 1.195 ± 0.05
2.391AsnPro: 2.391 ± 0.065
1.057AsnGln: 1.057 ± 0.045
1.988AsnArg: 1.988 ± 0.054
1.752AsnSer: 1.752 ± 0.073
2.189AsnThr: 2.189 ± 0.075
2.443AsnVal: 2.443 ± 0.071
0.334AsnTrp: 0.334 ± 0.03
0.997AsnTyr: 0.997 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
5.514ProAla: 5.514 ± 0.095
0.558ProCys: 0.558 ± 0.035
3.399ProAsp: 3.399 ± 0.074
3.68ProGlu: 3.68 ± 0.083
1.984ProPhe: 1.984 ± 0.056
4.716ProGly: 4.716 ± 0.101
0.984ProHis: 0.984 ± 0.036
2.257ProIle: 2.257 ± 0.063
1.811ProLys: 1.811 ± 0.049
4.267ProLeu: 4.267 ± 0.089
1.012ProMet: 1.012 ± 0.039
1.133ProAsn: 1.133 ± 0.039
2.242ProPro: 2.242 ± 0.063
1.493ProGln: 1.493 ± 0.052
2.09ProArg: 2.09 ± 0.056
2.67ProSer: 2.67 ± 0.07
2.582ProThr: 2.582 ± 0.097
5.242ProVal: 5.242 ± 0.095
0.448ProTrp: 0.448 ± 0.025
1.342ProTyr: 1.342 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.587GlnAla: 2.587 ± 0.063
0.323GlnCys: 0.323 ± 0.022
1.545GlnAsp: 1.545 ± 0.044
1.932GlnGlu: 1.932 ± 0.047
1.152GlnPhe: 1.152 ± 0.039
1.905GlnGly: 1.905 ± 0.055
0.594GlnHis: 0.594 ± 0.032
2.235GlnIle: 2.235 ± 0.061
1.87GlnLys: 1.87 ± 0.056
2.26GlnLeu: 2.26 ± 0.056
0.938GlnMet: 0.938 ± 0.038
1.169GlnAsn: 1.169 ± 0.051
1.229GlnPro: 1.229 ± 0.046
1.18GlnGln: 1.18 ± 0.042
1.482GlnArg: 1.482 ± 0.052
1.58GlnSer: 1.58 ± 0.054
1.629GlnThr: 1.629 ± 0.055
2.255GlnVal: 2.255 ± 0.054
0.296GlnTrp: 0.296 ± 0.025
1.136GlnTyr: 1.136 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
4.504ArgAla: 4.504 ± 0.103
0.803ArgCys: 0.803 ± 0.033
3.374ArgAsp: 3.374 ± 0.079
4.545ArgGlu: 4.545 ± 0.094
2.33ArgPhe: 2.33 ± 0.056
3.455ArgGly: 3.455 ± 0.071
1.328ArgHis: 1.328 ± 0.04
4.362ArgIle: 4.362 ± 0.088
3.011ArgLys: 3.011 ± 0.074
4.739ArgLeu: 4.739 ± 0.098
1.593ArgMet: 1.593 ± 0.047
1.782ArgAsn: 1.782 ± 0.046
2.3ArgPro: 2.3 ± 0.067
1.963ArgGln: 1.963 ± 0.053
3.034ArgArg: 3.034 ± 0.071
3.008ArgSer: 3.008 ± 0.065
2.869ArgThr: 2.869 ± 0.066
3.752ArgVal: 3.752 ± 0.076
0.575ArgTrp: 0.575 ± 0.03
2.081ArgTyr: 2.081 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
5.457SerAla: 5.457 ± 0.114
0.806SerCys: 0.806 ± 0.034
3.099SerAsp: 3.099 ± 0.06
2.979SerGlu: 2.979 ± 0.069
2.407SerPhe: 2.407 ± 0.064
6.126SerGly: 6.126 ± 0.121
1.259SerHis: 1.259 ± 0.045
3.518SerIle: 3.518 ± 0.081
1.934SerLys: 1.934 ± 0.06
5.738SerLeu: 5.738 ± 0.101
1.442SerMet: 1.442 ± 0.051
1.633SerAsn: 1.633 ± 0.06
3.463SerPro: 3.463 ± 0.088
1.665SerGln: 1.665 ± 0.059
3.484SerArg: 3.484 ± 0.087
4.043SerSer: 4.043 ± 0.108
3.601SerThr: 3.601 ± 0.089
4.487SerVal: 4.487 ± 0.091
0.72SerTrp: 0.72 ± 0.036
1.706SerTyr: 1.706 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
5.704ThrAla: 5.704 ± 0.13
0.813ThrCys: 0.813 ± 0.039
3.157ThrAsp: 3.157 ± 0.075
3.063ThrGlu: 3.063 ± 0.075
2.293ThrPhe: 2.293 ± 0.058
6.452ThrGly: 6.452 ± 0.12
1.118ThrHis: 1.118 ± 0.045
4.318ThrIle: 4.318 ± 0.08
2.37ThrLys: 2.37 ± 0.068
6.049ThrLeu: 6.049 ± 0.115
1.221ThrMet: 1.221 ± 0.046
1.841ThrAsn: 1.841 ± 0.07
3.653ThrPro: 3.653 ± 0.092
1.47ThrGln: 1.47 ± 0.05
3.033ThrArg: 3.033 ± 0.064
3.665ThrSer: 3.665 ± 0.107
4.087ThrThr: 4.087 ± 0.136
4.905ThrVal: 4.905 ± 0.102
0.583ThrTrp: 0.583 ± 0.031
1.927ThrTyr: 1.927 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
6.772ValAla: 6.772 ± 0.116
1.039ValCys: 1.039 ± 0.043
3.539ValAsp: 3.539 ± 0.078
3.833ValGlu: 3.833 ± 0.082
3.115ValPhe: 3.115 ± 0.062
4.588ValGly: 4.588 ± 0.099
1.504ValHis: 1.504 ± 0.047
5.986ValIle: 5.986 ± 0.095
3.374ValLys: 3.374 ± 0.073
7.282ValLeu: 7.282 ± 0.119
1.923ValMet: 1.923 ± 0.055
2.625ValAsn: 2.625 ± 0.075
4.251ValPro: 4.251 ± 0.094
2.352ValGln: 2.352 ± 0.059
4.079ValArg: 4.079 ± 0.084
4.799ValSer: 4.799 ± 0.08
5.341ValThr: 5.341 ± 0.108
5.814ValVal: 5.814 ± 0.099
0.683ValTrp: 0.683 ± 0.033
2.12ValTyr: 2.12 ± 0.066
0.0ValXaa: 0.0 ± 0.0
Trp
0.697TrpAla: 0.697 ± 0.035
0.154TrpCys: 0.154 ± 0.014
0.549TrpAsp: 0.549 ± 0.03
0.549TrpGlu: 0.549 ± 0.025
0.415TrpPhe: 0.415 ± 0.025
0.641TrpGly: 0.641 ± 0.029
0.184TrpHis: 0.184 ± 0.015
0.781TrpIle: 0.781 ± 0.03
0.542TrpLys: 0.542 ± 0.03
0.906TrpLeu: 0.906 ± 0.036
0.287TrpMet: 0.287 ± 0.019
0.522TrpAsn: 0.522 ± 0.034
0.242TrpPro: 0.242 ± 0.018
0.441TrpGln: 0.441 ± 0.025
0.502TrpArg: 0.502 ± 0.027
0.593TrpSer: 0.593 ± 0.036
0.514TrpThr: 0.514 ± 0.025
0.687TrpVal: 0.687 ± 0.033
0.151TrpTrp: 0.151 ± 0.015
0.437TrpTyr: 0.437 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.791TyrAla: 2.791 ± 0.068
0.47TyrCys: 0.47 ± 0.027
1.599TyrAsp: 1.599 ± 0.057
1.603TyrGlu: 1.603 ± 0.051
1.197TyrPhe: 1.197 ± 0.044
2.429TyrGly: 2.429 ± 0.062
0.671TyrHis: 0.671 ± 0.031
1.914TyrIle: 1.914 ± 0.056
0.969TyrLys: 0.969 ± 0.034
3.029TyrLeu: 3.029 ± 0.068
0.561TyrMet: 0.561 ± 0.026
1.015TyrAsn: 1.015 ± 0.046
1.519TyrPro: 1.519 ± 0.047
1.041TyrGln: 1.041 ± 0.04
2.04TyrArg: 2.04 ± 0.06
1.823TyrSer: 1.823 ± 0.057
1.989TyrThr: 1.989 ± 0.061
1.976TyrVal: 1.976 ± 0.059
0.326TyrTrp: 0.326 ± 0.024
1.097TyrTyr: 1.097 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2450 proteins (727371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski