Crassostrea gigas (Pacific oyster) (Crassostrea angulata)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Bivalvia; Autobranchia; Pteriomorphia; Ostreida; Ostreoidea; Ostreidae; Crassostrea

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K1Q4D5|K1Q4D5_CRAGI Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10014019 PE=4 SV=1
MM1 pKa = 8.2RR2 pKa = 11.84DD3 pKa = 3.33TLYY6 pKa = 11.03LPSQGDD12 pKa = 3.62MCEE15 pKa = 4.0SSPILMPGQQVQHH28 pKa = 6.82PAMLGLSAFEE38 pKa = 3.8QAEE41 pKa = 4.46VVLTGYY47 pKa = 10.22RR48 pKa = 11.84DD49 pKa = 3.57CAAEE53 pKa = 4.47AIRR56 pKa = 11.84YY57 pKa = 8.14LVEE60 pKa = 3.89EE61 pKa = 4.66EE62 pKa = 4.41RR63 pKa = 11.84FAMDD67 pKa = 3.86DD68 pKa = 3.52PLVIGLRR75 pKa = 11.84KK76 pKa = 9.42HH77 pKa = 6.55LYY79 pKa = 7.64EE80 pKa = 3.87QQRR83 pKa = 11.84ALNIHH88 pKa = 7.08RR89 pKa = 11.84ILSNLEE95 pKa = 3.6NNPYY99 pKa = 10.35YY100 pKa = 10.47DD101 pKa = 4.99DD102 pKa = 5.9EE103 pKa = 6.09IDD105 pKa = 3.49NTYY108 pKa = 11.03LDD110 pKa = 4.21DD111 pKa = 4.42SGIGEE116 pKa = 4.24TSIGGNGSSMEE127 pKa = 3.91GSTPSEE133 pKa = 4.04TDD135 pKa = 2.73SDD137 pKa = 4.18FLPSGNISNVTPEE150 pKa = 3.79VDD152 pKa = 2.88RR153 pKa = 11.84DD154 pKa = 4.3AIVSLAEE161 pKa = 4.67EE162 pKa = 4.05ILSLIEE168 pKa = 4.41LGEE171 pKa = 4.16EE172 pKa = 4.32PEE174 pKa = 4.65EE175 pKa = 4.51DD176 pKa = 3.39TDD178 pKa = 3.82GCPPVDD184 pKa = 3.38TDD186 pKa = 5.06DD187 pKa = 5.48YY188 pKa = 11.78LMQQ191 pKa = 5.35

Molecular weight: 21.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K1RG26|K1RG26_CRAGI MFS_1_like domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10016820 PE=3 SV=1
MM1 pKa = 7.04GTATDD6 pKa = 4.35CSRR9 pKa = 11.84SSLTPTEE16 pKa = 4.26PSRR19 pKa = 11.84HH20 pKa = 5.61ILPAKK25 pKa = 9.18LNPYY29 pKa = 9.1IVQFSRR35 pKa = 11.84ATCMARR41 pKa = 11.84TEE43 pKa = 4.19EE44 pKa = 4.32KK45 pKa = 10.78YY46 pKa = 10.42EE47 pKa = 4.07CRR49 pKa = 11.84LCFPSLTFDD58 pKa = 3.6RR59 pKa = 11.84KK60 pKa = 10.19FLVKK64 pKa = 10.28RR65 pKa = 11.84HH66 pKa = 6.02IMDD69 pKa = 3.21QHH71 pKa = 7.82SGFAYY76 pKa = 9.3TCTGCQMIFPRR87 pKa = 11.84RR88 pKa = 11.84DD89 pKa = 2.9NHH91 pKa = 5.42TSCHH95 pKa = 5.78GKK97 pKa = 9.37HH98 pKa = 5.35SRR100 pKa = 11.84SRR102 pKa = 11.84RR103 pKa = 11.84MEE105 pKa = 3.73VVRR108 pKa = 11.84RR109 pKa = 11.84SDD111 pKa = 3.21GLRR114 pKa = 11.84GSRR117 pKa = 11.84AQEE120 pKa = 3.79EE121 pKa = 4.62LEE123 pKa = 4.44KK124 pKa = 9.58YY125 pKa = 8.28TSRR128 pKa = 11.84IEE130 pKa = 3.96DD131 pKa = 4.45FIWVVSQRR139 pKa = 11.84GFRR142 pKa = 11.84EE143 pKa = 4.11LSLHH147 pKa = 5.43PSGRR151 pKa = 11.84ISRR154 pKa = 11.84TPYY157 pKa = 9.57PVARR161 pKa = 11.84PDD163 pKa = 3.45RR164 pKa = 11.84GTVPPSPRR172 pKa = 11.84ASPEE176 pKa = 3.56PKK178 pKa = 8.34RR179 pKa = 11.84TRR181 pKa = 11.84RR182 pKa = 11.84SRR184 pKa = 11.84RR185 pKa = 11.84SHH187 pKa = 6.48SPVNPPQEE195 pKa = 4.24RR196 pKa = 11.84QSPSPVALPARR207 pKa = 11.84KK208 pKa = 8.32RR209 pKa = 11.84TLSVSSSSSSSGSSSCSVAPEE230 pKa = 3.72NPIRR234 pKa = 11.84SSVVKK239 pKa = 10.24EE240 pKa = 3.63ITVVPGLRR248 pKa = 11.84VLIDD252 pKa = 3.05VDD254 pKa = 3.89EE255 pKa = 4.95FKK257 pKa = 11.1KK258 pKa = 10.37HH259 pKa = 5.2LL260 pKa = 4.06

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25998

0

25998

11654340

49

16310

448.3

50.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.414 ± 0.012

2.388 ± 0.018

5.848 ± 0.012

6.695 ± 0.026

3.912 ± 0.011

5.963 ± 0.021

2.414 ± 0.008

5.507 ± 0.012

6.77 ± 0.02

8.389 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.008

4.981 ± 0.012

4.795 ± 0.017

4.241 ± 0.013

5.016 ± 0.013

8.274 ± 0.021

6.1 ± 0.016

6.407 ± 0.015

1.102 ± 0.006

3.271 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski