Amino acid dipepetide frequency for Anopheles stephensi (Indo-Pakistan malaria mosquito)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.109AlaAla: 8.109 ± 0.087	1.163AlaCys: 1.163 ± 0.023	3.558AlaAsp: 3.558 ± 0.026	4.237AlaGlu: 4.237 ± 0.031	2.295AlaPhe: 2.295 ± 0.02	5.533AlaGly: 5.533 ± 0.047	1.628AlaHis: 1.628 ± 0.017	3.691AlaIle: 3.691 ± 0.03	3.87AlaLys: 3.87 ± 0.032	6.312AlaLeu: 6.312 ± 0.038	1.696AlaMet: 1.696 ± 0.018	3.324AlaAsn: 3.324 ± 0.022	3.795AlaPro: 3.795 ± 0.033	2.897AlaGln: 2.897 ± 0.024	3.513AlaArg: 3.513 ± 0.027	6.395AlaSer: 6.395 ± 0.046	5.036AlaThr: 5.036 ± 0.036	4.66AlaVal: 4.66 ± 0.032	0.578AlaTrp: 0.578 ± 0.01	1.824AlaTyr: 1.824 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	1.209CysAla: 1.209 ± 0.018	0.472CysCys: 0.472 ± 0.012	1.105CysAsp: 1.105 ± 0.021	1.078CysGlu: 1.078 ± 0.019	0.718CysPhe: 0.718 ± 0.012	1.312CysGly: 1.312 ± 0.026	0.459CysHis: 0.459 ± 0.009	0.966CysIle: 0.966 ± 0.019	0.872CysLys: 0.872 ± 0.017	1.66CysLeu: 1.66 ± 0.02	0.386CysMet: 0.386 ± 0.008	0.816CysAsn: 0.816 ± 0.018	1.009CysPro: 1.009 ± 0.026	0.685CysGln: 0.685 ± 0.016	1.092CysArg: 1.092 ± 0.026	1.518CysSer: 1.518 ± 0.028	1.075CysThr: 1.075 ± 0.021	1.222CysVal: 1.222 ± 0.023	0.221CysTrp: 0.221 ± 0.006	0.613CysTyr: 0.613 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.608AspAla: 3.608 ± 0.026	1.032AspCys: 1.032 ± 0.021	4.163AspAsp: 4.163 ± 0.043	4.428AspGlu: 4.428 ± 0.036	1.977AspPhe: 1.977 ± 0.019	3.981AspGly: 3.981 ± 0.032	1.215AspHis: 1.215 ± 0.015	2.709AspIle: 2.709 ± 0.024	2.436AspLys: 2.436 ± 0.026	4.479AspLeu: 4.479 ± 0.034	1.16AspMet: 1.16 ± 0.014	2.022AspAsn: 2.022 ± 0.02	2.63AspPro: 2.63 ± 0.027	1.879AspGln: 1.879 ± 0.017	3.195AspArg: 3.195 ± 0.029	4.025AspSer: 4.025 ± 0.033	2.793AspThr: 2.793 ± 0.021	3.366AspVal: 3.366 ± 0.026	0.586AspTrp: 0.586 ± 0.011	1.711AspTyr: 1.711 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	4.177GluAla: 4.177 ± 0.031	1.178GluCys: 1.178 ± 0.029	3.757GluAsp: 3.757 ± 0.034	5.608GluGlu: 5.608 ± 0.06	1.999GluPhe: 1.999 ± 0.019	2.993GluGly: 2.993 ± 0.025	1.559GluHis: 1.559 ± 0.016	3.087GluIle: 3.087 ± 0.027	3.897GluLys: 3.897 ± 0.043	6.077GluLeu: 6.077 ± 0.043	1.578GluMet: 1.578 ± 0.017	2.728GluAsn: 2.728 ± 0.024	2.939GluPro: 2.939 ± 0.038	3.351GluGln: 3.351 ± 0.033	4.139GluArg: 4.139 ± 0.035	4.233GluSer: 4.233 ± 0.031	3.386GluThr: 3.386 ± 0.03	3.388GluVal: 3.388 ± 0.028	0.595GluTrp: 0.595 ± 0.01	1.865GluTyr: 1.865 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.324PheAla: 2.324 ± 0.025	0.74PheCys: 0.74 ± 0.012	2.094PheAsp: 2.094 ± 0.018	2.068PheGlu: 2.068 ± 0.02	1.441PhePhe: 1.441 ± 0.02	2.512PheGly: 2.512 ± 0.028	0.937PheHis: 0.937 ± 0.013	1.8PheIle: 1.8 ± 0.022	1.632PheLys: 1.632 ± 0.018	3.362PheLeu: 3.362 ± 0.031	0.788PheMet: 0.788 ± 0.014	1.608PheAsn: 1.608 ± 0.017	1.609PhePro: 1.609 ± 0.02	1.369PheGln: 1.369 ± 0.016	2.087PheArg: 2.087 ± 0.021	2.584PheSer: 2.584 ± 0.024	1.935PheThr: 1.935 ± 0.021	2.503PheVal: 2.503 ± 0.024	0.413PheTrp: 0.413 ± 0.01	1.251PheTyr: 1.251 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	4.969GlyAla: 4.969 ± 0.044	1.075GlyCys: 1.075 ± 0.018	3.127GlyAsp: 3.127 ± 0.029	3.41GlyGlu: 3.41 ± 0.031	2.194GlyPhe: 2.194 ± 0.025	8.388GlyGly: 8.388 ± 0.12	1.602GlyHis: 1.602 ± 0.02	2.87GlyIle: 2.87 ± 0.024	3.367GlyLys: 3.367 ± 0.029	4.773GlyLeu: 4.773 ± 0.039	1.507GlyMet: 1.507 ± 0.021	2.938GlyAsn: 2.938 ± 0.025	3.036GlyPro: 3.036 ± 0.043	2.407GlyGln: 2.407 ± 0.025	3.392GlyArg: 3.392 ± 0.032	6.52GlySer: 6.52 ± 0.053	4.414GlyThr: 4.414 ± 0.03	3.855GlyVal: 3.855 ± 0.029	0.674GlyTrp: 0.674 ± 0.013	2.038GlyTyr: 2.038 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	1.542HisAla: 1.542 ± 0.018	0.556HisCys: 0.556 ± 0.01	1.174HisAsp: 1.174 ± 0.014	1.368HisGlu: 1.368 ± 0.015	0.971HisPhe: 0.971 ± 0.013	1.701HisGly: 1.701 ± 0.022	2.154HisHis: 2.154 ± 0.045	1.227HisIle: 1.227 ± 0.017	1.108HisLys: 1.108 ± 0.014	2.424HisLeu: 2.424 ± 0.022	0.584HisMet: 0.584 ± 0.012	1.107HisAsn: 1.107 ± 0.016	1.655HisPro: 1.655 ± 0.016	1.719HisGln: 1.719 ± 0.024	1.678HisArg: 1.678 ± 0.017	2.033HisSer: 2.033 ± 0.021	1.393HisThr: 1.393 ± 0.016	1.417HisVal: 1.417 ± 0.015	0.282HisTrp: 0.282 ± 0.006	0.928HisTyr: 0.928 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.682IleAla: 3.682 ± 0.026	1.032IleCys: 1.032 ± 0.019	2.973IleAsp: 2.973 ± 0.025	3.168IleGlu: 3.168 ± 0.029	1.837IlePhe: 1.837 ± 0.018	3.027IleGly: 3.027 ± 0.022	1.125IleHis: 1.125 ± 0.015	2.545IleIle: 2.545 ± 0.025	2.495IleLys: 2.495 ± 0.025	4.213IleLeu: 4.213 ± 0.034	1.056IleMet: 1.056 ± 0.012	2.26IleAsn: 2.26 ± 0.024	2.636IlePro: 2.636 ± 0.022	1.874IleGln: 1.874 ± 0.02	2.749IleArg: 2.749 ± 0.022	3.575IleSer: 3.575 ± 0.026	2.828IleThr: 2.828 ± 0.026	3.73IleVal: 3.73 ± 0.028	0.464IleTrp: 0.464 ± 0.01	1.487IleTyr: 1.487 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	3.452LysAla: 3.452 ± 0.029	0.967LysCys: 0.967 ± 0.019	2.737LysAsp: 2.737 ± 0.033	3.597LysGlu: 3.597 ± 0.035	1.721LysPhe: 1.721 ± 0.02	2.478LysGly: 2.478 ± 0.03	1.466LysHis: 1.466 ± 0.016	2.68LysIle: 2.68 ± 0.026	3.802LysLys: 3.802 ± 0.05	5.231LysLeu: 5.231 ± 0.037	1.314LysMet: 1.314 ± 0.015	2.149LysAsn: 2.149 ± 0.023	2.946LysPro: 2.946 ± 0.038	2.728LysGln: 2.728 ± 0.029	3.546LysArg: 3.546 ± 0.031	3.766LysSer: 3.766 ± 0.034	2.854LysThr: 2.854 ± 0.024	3.069LysVal: 3.069 ± 0.03	0.504LysTrp: 0.504 ± 0.01	1.688LysTyr: 1.688 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	6.609LeuAla: 6.609 ± 0.044	1.668LeuCys: 1.668 ± 0.021	4.748LeuAsp: 4.748 ± 0.035	5.804LeuGlu: 5.804 ± 0.044	3.237LeuPhe: 3.237 ± 0.034	4.708LeuGly: 4.708 ± 0.038	2.47LeuHis: 2.47 ± 0.023	4.075LeuIle: 4.075 ± 0.034	5.09LeuLys: 5.09 ± 0.038	9.151LeuLeu: 9.151 ± 0.068	2.03LeuMet: 2.03 ± 0.019	3.982LeuAsn: 3.982 ± 0.032	4.924LeuPro: 4.924 ± 0.033	4.679LeuGln: 4.679 ± 0.042	5.252LeuArg: 5.252 ± 0.04	6.646LeuSer: 6.646 ± 0.04	4.951LeuThr: 4.951 ± 0.028	5.47LeuVal: 5.47 ± 0.038	0.89LeuTrp: 0.89 ± 0.015	2.702LeuTyr: 2.702 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	1.86MetAla: 1.86 ± 0.017	0.392MetCys: 0.392 ± 0.009	1.288MetAsp: 1.288 ± 0.015	1.511MetGlu: 1.511 ± 0.016	0.869MetPhe: 0.869 ± 0.012	1.288MetGly: 1.288 ± 0.018	0.588MetHis: 0.588 ± 0.011	1.093MetIle: 1.093 ± 0.014	1.359MetLys: 1.359 ± 0.017	2.227MetLeu: 2.227 ± 0.023	0.716MetMet: 0.716 ± 0.012	1.026MetAsn: 1.026 ± 0.012	1.11MetPro: 1.11 ± 0.014	1.073MetGln: 1.073 ± 0.013	1.242MetArg: 1.242 ± 0.016	1.637MetSer: 1.637 ± 0.017	1.134MetThr: 1.134 ± 0.013	1.491MetVal: 1.491 ± 0.016	0.201MetTrp: 0.201 ± 0.005	0.69MetTyr: 0.69 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.209AsnAla: 3.209 ± 0.027	0.826AsnCys: 0.826 ± 0.016	2.387AsnAsp: 2.387 ± 0.02	2.697AsnGlu: 2.697 ± 0.025	1.589AsnPhe: 1.589 ± 0.019	3.847AsnGly: 3.847 ± 0.038	1.181AsnHis: 1.181 ± 0.017	2.267AsnIle: 2.267 ± 0.021	1.937AsnLys: 1.937 ± 0.022	3.874AsnLeu: 3.874 ± 0.031	0.972AsnMet: 0.972 ± 0.013	2.725AsnAsn: 2.725 ± 0.038	2.228AsnPro: 2.228 ± 0.026	1.78AsnGln: 1.78 ± 0.023	2.624AsnArg: 2.624 ± 0.022	3.568AsnSer: 3.568 ± 0.032	2.321AsnThr: 2.321 ± 0.022	3.107AsnVal: 3.107 ± 0.023	0.424AsnTrp: 0.424 ± 0.009	1.438AsnTyr: 1.438 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	4.54ProAla: 4.54 ± 0.038	0.749ProCys: 0.749 ± 0.031	2.705ProAsp: 2.705 ± 0.025	3.28ProGlu: 3.28 ± 0.034	1.726ProPhe: 1.726 ± 0.02	3.368ProGly: 3.368 ± 0.047	1.323ProHis: 1.323 ± 0.017	2.543ProIle: 2.543 ± 0.022	2.771ProLys: 2.771 ± 0.032	4.232ProLeu: 4.232 ± 0.032	1.146ProMet: 1.146 ± 0.015	2.39ProAsn: 2.39 ± 0.024	4.804ProPro: 4.804 ± 0.061	2.321ProGln: 2.321 ± 0.031	2.348ProArg: 2.348 ± 0.023	4.835ProSer: 4.835 ± 0.046	3.723ProThr: 3.723 ± 0.035	3.705ProVal: 3.705 ± 0.036	0.449ProTrp: 0.449 ± 0.009	1.563ProTyr: 1.563 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	2.803GlnAla: 2.803 ± 0.023	0.836GlnCys: 0.836 ± 0.018	1.856GlnAsp: 1.856 ± 0.019	2.703GlnGlu: 2.703 ± 0.028	1.492GlnPhe: 1.492 ± 0.017	1.871GlnGly: 1.871 ± 0.023	1.913GlnHis: 1.913 ± 0.028	2.083GlnIle: 2.083 ± 0.018	2.27GlnLys: 2.27 ± 0.024	4.63GlnLeu: 4.63 ± 0.033	1.119GlnMet: 1.119 ± 0.015	1.831GlnAsn: 1.831 ± 0.02	2.779GlnPro: 2.779 ± 0.036	7.063GlnGln: 7.063 ± 0.163	2.813GlnArg: 2.813 ± 0.024	3.226GlnSer: 3.226 ± 0.03	2.515GlnThr: 2.515 ± 0.021	2.121GlnVal: 2.121 ± 0.023	0.439GlnTrp: 0.439 ± 0.01	1.455GlnTyr: 1.455 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	3.487ArgAla: 3.487 ± 0.029	1.168ArgCys: 1.168 ± 0.021	2.906ArgAsp: 2.906 ± 0.029	3.571ArgGlu: 3.571 ± 0.032	2.181ArgPhe: 2.181 ± 0.022	2.816ArgGly: 2.816 ± 0.029	1.552ArgHis: 1.552 ± 0.017	2.812ArgIle: 2.812 ± 0.024	3.817ArgLys: 3.817 ± 0.033	5.078ArgLeu: 5.078 ± 0.037	1.372ArgMet: 1.372 ± 0.013	2.761ArgAsn: 2.761 ± 0.023	2.716ArgPro: 2.716 ± 0.027	2.568ArgGln: 2.568 ± 0.023	4.042ArgArg: 4.042 ± 0.042	4.441ArgSer: 4.441 ± 0.044	3.391ArgThr: 3.391 ± 0.028	2.999ArgVal: 2.999 ± 0.024	0.66ArgTrp: 0.66 ± 0.011	1.976ArgTyr: 1.976 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.107SerAla: 6.107 ± 0.04	1.39SerCys: 1.39 ± 0.027	4.092SerAsp: 4.092 ± 0.031	4.269SerGlu: 4.269 ± 0.036	2.846SerPhe: 2.846 ± 0.025	5.977SerGly: 5.977 ± 0.051	1.802SerHis: 1.802 ± 0.018	3.887SerIle: 3.887 ± 0.03	3.926SerLys: 3.926 ± 0.031	6.565SerLeu: 6.565 ± 0.039	1.74SerMet: 1.74 ± 0.017	4.065SerAsn: 4.065 ± 0.033	4.464SerPro: 4.464 ± 0.052	2.907SerGln: 2.907 ± 0.025	3.854SerArg: 3.854 ± 0.037	10.59SerSer: 10.59 ± 0.132	5.619SerThr: 5.619 ± 0.047	5.079SerVal: 5.079 ± 0.034	0.716SerTrp: 0.716 ± 0.011	2.254SerTyr: 2.254 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.029ThrAla: 5.029 ± 0.039	0.998ThrCys: 0.998 ± 0.019	2.991ThrAsp: 2.991 ± 0.021	3.384ThrGlu: 3.384 ± 0.03	2.063ThrPhe: 2.063 ± 0.02	4.173ThrGly: 4.173 ± 0.035	1.29ThrHis: 1.29 ± 0.016	3.416ThrIle: 3.416 ± 0.024	2.847ThrLys: 2.847 ± 0.025	5.26ThrLeu: 5.26 ± 0.033	1.336ThrMet: 1.336 ± 0.014	2.724ThrAsn: 2.724 ± 0.021	3.644ThrPro: 3.644 ± 0.035	2.004ThrGln: 2.004 ± 0.019	2.573ThrArg: 2.573 ± 0.023	5.003ThrSer: 5.003 ± 0.04	5.398ThrThr: 5.398 ± 0.097	4.711ThrVal: 4.711 ± 0.035	0.469ThrTrp: 0.469 ± 0.01	1.536ThrTyr: 1.536 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	4.946ValAla: 4.946 ± 0.031	1.349ValCys: 1.349 ± 0.023	3.294ValAsp: 3.294 ± 0.025	3.888ValGlu: 3.888 ± 0.038	2.241ValPhe: 2.241 ± 0.023	3.907ValGly: 3.907 ± 0.029	1.564ValHis: 1.564 ± 0.017	2.941ValIle: 2.941 ± 0.025	3.225ValLys: 3.225 ± 0.028	5.848ValLeu: 5.848 ± 0.031	1.359ValMet: 1.359 ± 0.016	2.556ValAsn: 2.556 ± 0.022	3.806ValPro: 3.806 ± 0.031	2.776ValGln: 2.776 ± 0.023	3.547ValArg: 3.547 ± 0.029	4.681ValSer: 4.681 ± 0.03	3.824ValThr: 3.824 ± 0.032	4.357ValVal: 4.357 ± 0.026	0.639ValTrp: 0.639 ± 0.011	1.882ValTyr: 1.882 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.527TrpAla: 0.527 ± 0.01	0.207TrpCys: 0.207 ± 0.006	0.536TrpAsp: 0.536 ± 0.011	0.549TrpGlu: 0.549 ± 0.009	0.403TrpPhe: 0.403 ± 0.008	0.508TrpGly: 0.508 ± 0.011	0.257TrpHis: 0.257 ± 0.006	0.599TrpIle: 0.599 ± 0.011	0.596TrpLys: 0.596 ± 0.012	0.973TrpLeu: 0.973 ± 0.015	0.284TrpMet: 0.284 ± 0.007	0.555TrpAsn: 0.555 ± 0.01	0.383TrpPro: 0.383 ± 0.008	0.412TrpGln: 0.412 ± 0.009	0.601TrpArg: 0.601 ± 0.011	0.711TrpSer: 0.711 ± 0.012	0.59TrpThr: 0.59 ± 0.01	0.513TrpVal: 0.513 ± 0.009	0.156TrpTrp: 0.156 ± 0.005	0.354TrpTyr: 0.354 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.923TyrAla: 1.923 ± 0.021	0.69TyrCys: 0.69 ± 0.012	1.905TyrAsp: 1.905 ± 0.021	1.917TyrGlu: 1.917 ± 0.02	1.252TyrPhe: 1.252 ± 0.018	2.172TyrGly: 2.172 ± 0.021	0.937TyrHis: 0.937 ± 0.012	1.464TyrIle: 1.464 ± 0.017	1.378TyrLys: 1.378 ± 0.019	2.716TyrLeu: 2.716 ± 0.024	0.653TyrMet: 0.653 ± 0.011	1.431TyrAsn: 1.431 ± 0.018	1.409TyrPro: 1.409 ± 0.019	1.326TyrGln: 1.326 ± 0.016	2.024TyrArg: 2.024 ± 0.022	2.166TyrSer: 2.166 ± 0.021	1.668TyrThr: 1.668 ± 0.021	1.868TyrVal: 1.868 ± 0.02	0.356TyrTrp: 0.356 ± 0.01	1.251TyrTyr: 1.251 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 11765 proteins (6746010 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---