Avon-Heathcote Estuary associated circular virus 15

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IB41|A0A0C5IB41_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 15 OX=1618238 PE=3 SV=1
MM1 pKa = 7.61HH2 pKa = 7.1NTTALQSSYY11 pKa = 10.8PNRR14 pKa = 11.84NSRR17 pKa = 11.84SPTANRR23 pKa = 11.84DD24 pKa = 3.09GAIRR28 pKa = 11.84NQSPRR33 pKa = 11.84TQQRR37 pKa = 11.84LSRR40 pKa = 11.84FVFTLNNWTEE50 pKa = 3.8AEE52 pKa = 4.21YY53 pKa = 10.93TDD55 pKa = 5.74IIASDD60 pKa = 4.0VKK62 pKa = 9.59WLVVGKK68 pKa = 10.15EE69 pKa = 3.88IGDD72 pKa = 3.59EE73 pKa = 4.32GTPHH77 pKa = 6.39LQGAVVIGKK86 pKa = 9.26QIAFSTVKK94 pKa = 9.82KK95 pKa = 9.71WPGFSRR101 pKa = 11.84AHH103 pKa = 5.99IEE105 pKa = 3.95NMRR108 pKa = 11.84GKK110 pKa = 9.71PFDD113 pKa = 3.55SLKK116 pKa = 10.9YY117 pKa = 9.23CTKK120 pKa = 10.17QDD122 pKa = 2.72TDD124 pKa = 3.85AFCKK128 pKa = 10.89GEE130 pKa = 4.0MPQEE134 pKa = 4.21GKK136 pKa = 10.82RR137 pKa = 11.84NDD139 pKa = 3.18LHH141 pKa = 7.36DD142 pKa = 5.73AINALKK148 pKa = 10.56QGGTLTSIVKK158 pKa = 10.36SDD160 pKa = 3.96DD161 pKa = 3.27VGLAAAVARR170 pKa = 11.84YY171 pKa = 9.68SKK173 pKa = 10.37GLAMVSSMLKK183 pKa = 9.68PRR185 pKa = 11.84RR186 pKa = 11.84SEE188 pKa = 3.84PPLVIWLSGEE198 pKa = 4.0TGVGKK203 pKa = 8.49TRR205 pKa = 11.84SAVALADD212 pKa = 5.22RR213 pKa = 11.84IAPDD217 pKa = 4.04NYY219 pKa = 9.27WISAGSLRR227 pKa = 11.84WFDD230 pKa = 4.57GYY232 pKa = 10.89DD233 pKa = 3.14GHH235 pKa = 8.5AIAIFDD241 pKa = 4.41DD242 pKa = 4.2LRR244 pKa = 11.84TKK246 pKa = 10.37HH247 pKa = 6.59AEE249 pKa = 3.69FSFLLRR255 pKa = 11.84LLDD258 pKa = 4.3RR259 pKa = 11.84YY260 pKa = 9.37PLRR263 pKa = 11.84VEE265 pKa = 4.48FKK267 pKa = 10.9GGFIDD272 pKa = 3.53WTPSVIVVTAPKK284 pKa = 10.33SPTHH288 pKa = 5.26MWSLRR293 pKa = 11.84NDD295 pKa = 3.27EE296 pKa = 5.6DD297 pKa = 3.61KK298 pKa = 11.24QQLEE302 pKa = 4.26RR303 pKa = 11.84RR304 pKa = 11.84IHH306 pKa = 5.73LAYY309 pKa = 10.36DD310 pKa = 3.44AEE312 pKa = 4.81EE313 pKa = 5.1GDD315 pKa = 3.94DD316 pKa = 4.22WEE318 pKa = 4.78LLLAKK323 pKa = 10.25SEE325 pKa = 4.59AIVAEE330 pKa = 4.54RR331 pKa = 11.84WPTGSYY337 pKa = 8.75PWKK340 pKa = 10.5HH341 pKa = 5.4YY342 pKa = 11.24DD343 pKa = 3.49EE344 pKa = 5.27VNVGSDD350 pKa = 2.93EE351 pKa = 4.17TTIPEE356 pKa = 4.19EE357 pKa = 4.36RR358 pKa = 11.84SCSLSTMGQEE368 pKa = 4.66PDD370 pKa = 3.32TSPSIIEE377 pKa = 4.15IEE379 pKa = 4.05SDD381 pKa = 3.35LSSEE385 pKa = 4.14EE386 pKa = 4.16LVISSSEE393 pKa = 4.07EE394 pKa = 3.53EE395 pKa = 4.18HH396 pKa = 7.74SYY398 pKa = 8.57EE399 pKa = 4.04TQRR402 pKa = 11.84EE403 pKa = 4.34LFSTQYY409 pKa = 11.18EE410 pKa = 4.53KK411 pKa = 10.7II412 pKa = 3.73

Molecular weight: 46.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IB41|A0A0C5IB41_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 15 OX=1618238 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.47RR4 pKa = 11.84SYY6 pKa = 11.59NFGTNPSQQYY16 pKa = 9.57GVSRR20 pKa = 11.84ATKK23 pKa = 9.61RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84NVNRR30 pKa = 11.84YY31 pKa = 7.75QNLPRR36 pKa = 11.84GMNQRR41 pKa = 11.84QIIRR45 pKa = 11.84PYY47 pKa = 10.33RR48 pKa = 11.84LYY50 pKa = 10.56TPRR53 pKa = 11.84TPGGQVIAEE62 pKa = 3.82RR63 pKa = 11.84KK64 pKa = 9.91YY65 pKa = 10.91FDD67 pKa = 3.25EE68 pKa = 4.16TLAAKK73 pKa = 9.98IVIAAVTDD81 pKa = 4.06FNDD84 pKa = 3.89AEE86 pKa = 4.11VDD88 pKa = 3.62PAVRR92 pKa = 11.84LCLFAPTQGNDD103 pKa = 2.48ISNRR107 pKa = 11.84EE108 pKa = 3.91SRR110 pKa = 11.84SCFVYY115 pKa = 10.63SITVRR120 pKa = 11.84GSIEE124 pKa = 3.88MLGQGQQTAIDD135 pKa = 3.81NNQIARR141 pKa = 11.84LVLVLDD147 pKa = 3.82KK148 pKa = 10.19QTNGTQMSSEE158 pKa = 4.49DD159 pKa = 4.05LLTTNSGEE167 pKa = 4.22SPMVYY172 pKa = 10.06AYY174 pKa = 11.04QNTANFGRR182 pKa = 11.84FQILKK187 pKa = 9.25DD188 pKa = 3.33QFIEE192 pKa = 4.96FEE194 pKa = 4.12PCNIGGVTGSFWQGGGRR211 pKa = 11.84KK212 pKa = 7.89MFKK215 pKa = 10.22LKK217 pKa = 9.19HH218 pKa = 5.02TFKK221 pKa = 11.06EE222 pKa = 4.37PIRR225 pKa = 11.84VNFGSTNGGTVADD238 pKa = 3.5IVDD241 pKa = 3.62NSFHH245 pKa = 6.97IICGTQNASITTVLNYY261 pKa = 9.91KK262 pKa = 10.18SRR264 pKa = 11.84VSFTGG269 pKa = 3.05

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

681

269

412

340.5

38.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.608 ± 0.56

1.028 ± 0.249

5.727 ± 0.89

6.314 ± 1.411

4.112 ± 0.796

7.195 ± 0.736

1.762 ± 0.553

6.167 ± 0.285

4.846 ± 0.411

6.755 ± 0.842

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.175

4.993 ± 1.529

4.405 ± 0.374

4.699 ± 1.082

6.902 ± 0.492

8.664 ± 0.869

7.048 ± 0.614

5.874 ± 0.444

1.762 ± 0.755

3.231 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski