Klebsiella phage Matisse

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Slopekvirus; Klebsiella virus Matisse

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 280 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LQC2|A0A0K1LQC2_9CAUD Head assembly protein OS=Klebsiella phage Matisse OX=1675607 GN=CPT_Matisse36 PE=4 SV=1
MM1 pKa = 7.7INLDD5 pKa = 3.44FCKK8 pKa = 10.88AEE10 pKa = 4.03MQADD14 pKa = 3.72ADD16 pKa = 3.98EE17 pKa = 4.99EE18 pKa = 4.45EE19 pKa = 4.65PKK21 pKa = 10.41FDD23 pKa = 4.2SPHH26 pKa = 5.84SSRR29 pKa = 11.84WIVAVDD35 pKa = 3.36DD36 pKa = 4.18EE37 pKa = 5.63GYY39 pKa = 8.7VTVLSRR45 pKa = 11.84PFIHH49 pKa = 7.68DD50 pKa = 3.38AFFEE54 pKa = 4.47CGRR57 pKa = 11.84SAEE60 pKa = 4.86DD61 pKa = 2.84IGLPDD66 pKa = 4.08SVEE69 pKa = 4.18MPPGVYY75 pKa = 9.52EE76 pKa = 4.23WICGFEE82 pKa = 4.0QTTDD86 pKa = 2.87WEE88 pKa = 4.3TGYY91 pKa = 11.05VDD93 pKa = 3.11GWEE96 pKa = 4.47FYY98 pKa = 10.33PNEE101 pKa = 4.04STLLYY106 pKa = 9.89SWEE109 pKa = 3.95NGKK112 pKa = 10.18VNEE115 pKa = 4.75SGNQEE120 pKa = 4.11TQSDD124 pKa = 4.0NGSGSLL130 pKa = 4.04

Molecular weight: 14.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LPL7|A0A0K1LPL7_9CAUD Uncharacterized protein OS=Klebsiella phage Matisse OX=1675607 GN=CPT_Matisse158 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.17RR3 pKa = 11.84SYY5 pKa = 11.65NMNEE9 pKa = 3.59LVFFKK14 pKa = 10.23TRR16 pKa = 11.84RR17 pKa = 11.84EE18 pKa = 3.6ARR20 pKa = 11.84AYY22 pKa = 10.51VLAAGKK28 pKa = 7.95NTSAAIDD35 pKa = 3.7MGTDD39 pKa = 3.15KK40 pKa = 11.32AVGARR45 pKa = 11.84WAAVIVIAAPVTPVKK60 pKa = 10.13TLVLGARR67 pKa = 11.84HH68 pKa = 6.69AEE70 pKa = 4.22TLNTAASGRR79 pKa = 11.84GHH81 pKa = 5.46EE82 pKa = 4.17VKK84 pKa = 10.57VFKK87 pKa = 10.58KK88 pKa = 10.43RR89 pKa = 11.84SYY91 pKa = 10.47LSCARR96 pKa = 11.84GG97 pKa = 3.1

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

280

0

280

55911

26

1349

199.7

22.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.154

1.225 ± 0.064

6.611 ± 0.103

6.915 ± 0.176

4.121 ± 0.089

6.283 ± 0.169

1.771 ± 0.08

6.736 ± 0.119

7.405 ± 0.195

7.453 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.079

5.48 ± 0.122

3.538 ± 0.069

3.255 ± 0.094

4.745 ± 0.11

6.051 ± 0.133

5.933 ± 0.189

6.966 ± 0.137

1.332 ± 0.045

4.516 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski