Klebsiella phage KPN U2874

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Yonseivirus; unclassified Yonseivirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286MMU1|A0A286MMU1_9CAUD Uncharacterized protein OS=Klebsiella phage KPN U2874 OX=2024241 GN=KPNU2874_10 PE=4 SV=1
MM1 pKa = 7.36ASVWFLVVTLCTVNPGALASDD22 pKa = 3.89CDD24 pKa = 4.44DD25 pKa = 3.71YY26 pKa = 12.11VIDD29 pKa = 5.25GDD31 pKa = 5.16LSYY34 pKa = 11.42SDD36 pKa = 5.1CIKK39 pKa = 10.82SIAQFPEE46 pKa = 3.79KK47 pKa = 10.37AALFAVSCKK56 pKa = 10.19RR57 pKa = 11.84GEE59 pKa = 4.12PLDD62 pKa = 4.02GVGGEE67 pKa = 4.2MM68 pKa = 5.09

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286MMY6|A0A286MMY6_9CAUD Uncharacterized protein OS=Klebsiella phage KPN U2874 OX=2024241 GN=KPNU2874_55 PE=4 SV=1
MM1 pKa = 7.39ASKK4 pKa = 10.48RR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.47SCEE13 pKa = 3.7GKK15 pKa = 9.58QRR17 pKa = 11.84YY18 pKa = 8.28PDD20 pKa = 3.4IPAALAGIRR29 pKa = 11.84YY30 pKa = 9.2IKK32 pKa = 9.46RR33 pKa = 11.84TYY35 pKa = 10.42GYY37 pKa = 9.23SGPMDD42 pKa = 5.03AYY44 pKa = 10.55HH45 pKa = 7.16CKK47 pKa = 10.54LCGKK51 pKa = 9.47IHH53 pKa = 6.95IGHH56 pKa = 5.92QKK58 pKa = 10.58GIGSHH63 pKa = 6.17RR64 pKa = 11.84PMGRR68 pKa = 11.84KK69 pKa = 8.69

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

18413

41

1437

230.2

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.319 ± 0.407

1.026 ± 0.125

6.561 ± 0.193

6.045 ± 0.3

3.546 ± 0.185

7.549 ± 0.265

1.526 ± 0.138

5.355 ± 0.166

5.398 ± 0.373

7.707 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.141

4.182 ± 0.193

4.339 ± 0.299

3.503 ± 0.351

6.191 ± 0.243

5.415 ± 0.272

5.947 ± 0.247

7.115 ± 0.192

1.923 ± 0.179

3.356 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski