Escherichia phage Mt1B1_P10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Vectrevirus; unclassified Vectrevirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H0XCA2|A0A7H0XCA2_9CAUD Uncharacterized protein OS=Escherichia phage Mt1B1_P10 OX=2743960 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.02YY9 pKa = 10.68NLLAATVEE17 pKa = 4.22AFNEE21 pKa = 4.26RR22 pKa = 11.84IKK24 pKa = 10.94YY25 pKa = 10.29DD26 pKa = 4.13EE27 pKa = 4.11IRR29 pKa = 11.84EE30 pKa = 4.15DD31 pKa = 4.41DD32 pKa = 4.57DD33 pKa = 5.3YY34 pKa = 12.04SDD36 pKa = 4.39ALHH39 pKa = 6.34EE40 pKa = 4.6VVDD43 pKa = 3.79SHH45 pKa = 7.75VPVYY49 pKa = 10.35YY50 pKa = 10.66SEE52 pKa = 4.53IFTVMAADD60 pKa = 5.26GIDD63 pKa = 3.82VEE65 pKa = 5.34FEE67 pKa = 4.13DD68 pKa = 5.51AGLIPDD74 pKa = 4.15TKK76 pKa = 10.54DD77 pKa = 2.9VTMILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.09ALYY91 pKa = 10.89NDD93 pKa = 4.1VPNDD97 pKa = 3.65SDD99 pKa = 4.27VVWYY103 pKa = 10.1EE104 pKa = 3.97DD105 pKa = 3.82EE106 pKa = 4.55EE107 pKa = 4.43EE108 pKa = 4.19EE109 pKa = 5.07KK110 pKa = 11.16

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H0XC98|A0A7H0XC98_9CAUD DNA-directed RNA polymerase OS=Escherichia phage Mt1B1_P10 OX=2743960 PE=3 SV=1
MM1 pKa = 7.19RR2 pKa = 11.84HH3 pKa = 5.3VYY5 pKa = 9.79VNGMLHH11 pKa = 5.92VVGKK15 pKa = 10.52RR16 pKa = 11.84KK17 pKa = 9.37GHH19 pKa = 4.96SRR21 pKa = 11.84RR22 pKa = 11.84NGLLILCEE30 pKa = 4.39AIVRR34 pKa = 11.84YY35 pKa = 9.5

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13507

29

1102

250.1

27.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.003 ± 0.445

1.096 ± 0.174

6.108 ± 0.208

6.885 ± 0.37

3.665 ± 0.136

7.833 ± 0.373

2.169 ± 0.239

5.271 ± 0.24

6.574 ± 0.302

7.863 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.006 ± 0.168

4.331 ± 0.253

3.606 ± 0.191

4.205 ± 0.245

5.434 ± 0.267

5.701 ± 0.264

5.397 ± 0.219

6.782 ± 0.279

1.466 ± 0.111

3.606 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski