Peptoniphilus sp. ING2-D1G

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; unclassified Peptoniphilus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090I1X4|A0A090I1X4_9FIRM HAD-superfamily hydrolase subfamily IB OS=Peptoniphilus sp. ING2-D1G OX=1912856 GN=ING2D1G_0431 PE=4 SV=1
MM1 pKa = 7.88DD2 pKa = 5.02FDD4 pKa = 5.55EE5 pKa = 5.83SLQQYY10 pKa = 8.28TYY12 pKa = 10.72AYY14 pKa = 9.05EE15 pKa = 4.3DD16 pKa = 5.28LIFVWDD22 pKa = 4.4EE23 pKa = 3.81LPKK26 pKa = 10.9EE27 pKa = 4.12NFHH30 pKa = 7.25EE31 pKa = 4.32IVEE34 pKa = 4.33VLADD38 pKa = 3.78SYY40 pKa = 11.75HH41 pKa = 6.5EE42 pKa = 4.37NFDD45 pKa = 4.46GILDD49 pKa = 4.8FILPNLRR56 pKa = 11.84EE57 pKa = 3.91IYY59 pKa = 10.87GDD61 pKa = 3.39VTRR64 pKa = 11.84EE65 pKa = 3.82DD66 pKa = 3.79VEE68 pKa = 4.38NKK70 pKa = 9.35IGKK73 pKa = 9.03PIINPEE79 pKa = 4.12NEE81 pKa = 4.03TVTYY85 pKa = 10.1CDD87 pKa = 3.1QSFDD91 pKa = 3.11SWHH94 pKa = 6.31IFTFEE99 pKa = 3.76FSNNNFEE106 pKa = 4.38DD107 pKa = 3.71LNYY110 pKa = 10.5FSVDD114 pKa = 3.05GG115 pKa = 4.3

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090JMI5|A0A090JMI5_9FIRM Branched-chain amino acid transport system carrier protein OS=Peptoniphilus sp. ING2-D1G OX=1912856 GN=brnQ PE=3 SV=1
MM1 pKa = 7.48SKK3 pKa = 10.6NLIKK7 pKa = 10.82NLSRR11 pKa = 11.84KK12 pKa = 9.14IKK14 pKa = 9.46LAKK17 pKa = 9.55FARR20 pKa = 11.84KK21 pKa = 9.54FSISTVKK28 pKa = 10.4LGKK31 pKa = 9.78IAFAVSRR38 pKa = 11.84IQKK41 pKa = 9.53RR42 pKa = 11.84NKK44 pKa = 10.11SKK46 pKa = 11.15

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1528

0

1528

474248

30

1929

310.4

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.0 ± 0.075

0.853 ± 0.023

6.064 ± 0.055

7.944 ± 0.076

4.542 ± 0.051

6.465 ± 0.069

1.309 ± 0.021

9.528 ± 0.066

8.804 ± 0.076

9.073 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.029

5.733 ± 0.054

2.869 ± 0.035

2.288 ± 0.028

3.649 ± 0.04

6.282 ± 0.043

4.816 ± 0.044

6.427 ± 0.054

0.539 ± 0.018

4.196 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski