Pseudoruegeria aquimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pseudoruegeria

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5SJ97|A0A1Y5SJ97_9RHOB Paraquat-inducible protein A OS=Pseudoruegeria aquimaris OX=393663 GN=PSA7680_02097 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.48LRR4 pKa = 11.84TALAATAAAFLAAPLSAQSLDD25 pKa = 3.98DD26 pKa = 3.54FTGFYY31 pKa = 10.7AGAQLGYY38 pKa = 11.05GDD40 pKa = 4.7VGTNAPGVSGDD51 pKa = 3.49DD52 pKa = 3.58WIGGIILGYY61 pKa = 10.67DD62 pKa = 3.46YY63 pKa = 11.57DD64 pKa = 4.22FGDD67 pKa = 3.66WVIGGGIDD75 pKa = 4.98YY76 pKa = 10.34DD77 pKa = 3.45WADD80 pKa = 3.17INIAAGVDD88 pKa = 3.87VEE90 pKa = 4.81SVWRR94 pKa = 11.84IKK96 pKa = 10.93ARR98 pKa = 11.84GGYY101 pKa = 9.06NWDD104 pKa = 3.26NSLIYY109 pKa = 9.57ATAGYY114 pKa = 11.0ADD116 pKa = 4.62AGTNALGSSDD126 pKa = 3.15GWLAGIGYY134 pKa = 7.77EE135 pKa = 4.01YY136 pKa = 10.45RR137 pKa = 11.84VRR139 pKa = 11.84SDD141 pKa = 3.39LSLGAEE147 pKa = 4.14LLYY150 pKa = 11.07HH151 pKa = 6.66EE152 pKa = 5.67FDD154 pKa = 4.55DD155 pKa = 5.34FNGSGIDD162 pKa = 3.5VDD164 pKa = 3.56ATTVQFRR171 pKa = 11.84ASYY174 pKa = 10.58RR175 pKa = 11.84FF176 pKa = 3.25

Molecular weight: 18.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5TMK4|A0A1Y5TMK4_9RHOB Sulfite reductase [NADPH] flavoprotein alpha-component OS=Pseudoruegeria aquimaris OX=393663 GN=cysJ PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 3.26VRR4 pKa = 11.84ITIEE8 pKa = 3.95TTFDD12 pKa = 3.06NGEE15 pKa = 4.14KK16 pKa = 9.26RR17 pKa = 11.84THH19 pKa = 5.66QLDD22 pKa = 4.77GISRR26 pKa = 11.84PYY28 pKa = 10.47RR29 pKa = 11.84VTCPDD34 pKa = 4.3GIGLRR39 pKa = 11.84LEE41 pKa = 4.27DD42 pKa = 3.35GKK44 pKa = 11.2RR45 pKa = 11.84VVEE48 pKa = 4.09QIQRR52 pKa = 11.84AILCDD57 pKa = 3.41QVEE60 pKa = 4.41EE61 pKa = 4.57IIRR64 pKa = 11.84EE65 pKa = 4.09SRR67 pKa = 11.84VCPDD71 pKa = 3.56CASVRR76 pKa = 11.84AIHH79 pKa = 7.56DD80 pKa = 3.39YY81 pKa = 9.3RR82 pKa = 11.84TRR84 pKa = 11.84DD85 pKa = 3.24LDD87 pKa = 3.78TLFGRR92 pKa = 11.84VRR94 pKa = 11.84VKK96 pKa = 10.21AARR99 pKa = 11.84LRR101 pKa = 11.84RR102 pKa = 11.84CSCDD106 pKa = 3.03ARR108 pKa = 11.84SAAMPGGPISPLAYY122 pKa = 10.02FFPDD126 pKa = 3.02RR127 pKa = 11.84ATPEE131 pKa = 4.12LQRR134 pKa = 11.84LHH136 pKa = 7.1AEE138 pKa = 3.85LGSRR142 pKa = 11.84HH143 pKa = 5.63SFRR146 pKa = 11.84EE147 pKa = 3.57AARR150 pKa = 11.84LMKK153 pKa = 10.46SFLPCHH159 pKa = 6.43PPHH162 pKa = 6.48HH163 pKa = 5.67TTVRR167 pKa = 11.84DD168 pKa = 3.25RR169 pKa = 11.84LGRR172 pKa = 11.84VAAGLEE178 pKa = 3.9KK179 pKa = 10.48SRR181 pKa = 11.84RR182 pKa = 11.84ASGDD186 pKa = 3.25PAEE189 pKa = 4.76ALPKK193 pKa = 10.75GGLTVFLDD201 pKa = 4.46GAHH204 pKa = 5.95IRR206 pKa = 11.84CRR208 pKa = 11.84PEE210 pKa = 3.34YY211 pKa = 8.69QQRR214 pKa = 11.84HH215 pKa = 5.75LDD217 pKa = 3.71LVVGKK222 pKa = 10.23IEE224 pKa = 4.05SRR226 pKa = 11.84NMCRR230 pKa = 11.84RR231 pKa = 11.84FGLVANATASPGSRR245 pKa = 11.84MRR247 pKa = 11.84EE248 pKa = 3.94EE249 pKa = 4.71LSDD252 pKa = 4.3FGWKK256 pKa = 9.57PGRR259 pKa = 11.84LLTVISDD266 pKa = 4.24GEE268 pKa = 4.09LALPNLIRR276 pKa = 11.84NAMGGDD282 pKa = 3.57GQVKK286 pKa = 10.25HH287 pKa = 6.82ILDD290 pKa = 3.19WWHH293 pKa = 6.44ISMRR297 pKa = 11.84IRR299 pKa = 11.84HH300 pKa = 5.41VEE302 pKa = 3.66AAVQGLVQIPGFTGIPVLFQRR323 pKa = 11.84PAKK326 pKa = 9.75SLRR329 pKa = 11.84WWLWHH334 pKa = 4.56GRR336 pKa = 11.84ARR338 pKa = 11.84VAEE341 pKa = 4.41TYY343 pKa = 10.85LKK345 pKa = 11.14GLMHH349 pKa = 7.53DD350 pKa = 3.96CTRR353 pKa = 11.84LAEE356 pKa = 4.24EE357 pKa = 4.18PLAVRR362 pKa = 11.84TAAARR367 pKa = 11.84VQARR371 pKa = 11.84CGTLYY376 pKa = 10.5TYY378 pKa = 10.54LANNMEE384 pKa = 4.33SLVDD388 pKa = 3.08YY389 pKa = 10.45GRR391 pKa = 11.84RR392 pKa = 11.84YY393 pKa = 10.55RR394 pKa = 11.84NGLPISSSRR403 pKa = 11.84AEE405 pKa = 4.1GSVDD409 pKa = 3.98DD410 pKa = 4.45IANARR415 pKa = 11.84MGKK418 pKa = 8.48RR419 pKa = 11.84RR420 pKa = 11.84RR421 pKa = 11.84MRR423 pKa = 11.84WSPKK427 pKa = 7.73GAHH430 pKa = 5.52RR431 pKa = 11.84VAVTRR436 pKa = 11.84AAVLDD441 pKa = 3.7GRR443 pKa = 11.84LTVANRR449 pKa = 11.84KK450 pKa = 8.71RR451 pKa = 11.84AAA453 pKa = 3.39

Molecular weight: 50.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3590

0

3590

1108921

32

1812

308.9

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.171 ± 0.061

0.924 ± 0.012

5.522 ± 0.038

6.451 ± 0.038

3.79 ± 0.028

8.898 ± 0.04

2.024 ± 0.02

4.953 ± 0.031

3.152 ± 0.034

10.126 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.02

2.438 ± 0.021

5.314 ± 0.03

3.006 ± 0.021

6.875 ± 0.048

4.838 ± 0.025

5.139 ± 0.021

7.079 ± 0.033

1.397 ± 0.018

2.238 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski