Gracilariopsis chorda

Taxonomy: cellular organisms; Eukaryota; Rhodophyta; Florideophyceae; Rhodymeniophycidae; Gracilariales; Gracilariaceae; Gracilariopsis

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3ICN1|A0A2V3ICN1_9FLOR Uncharacterized protein OS=Gracilariopsis chorda OX=448386 GN=BWQ96_10456 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.75GDD4 pKa = 4.72AEE6 pKa = 4.98GEE8 pKa = 4.11QCDD11 pKa = 4.5AISPAKK17 pKa = 9.13TQDD20 pKa = 3.17TASQDD25 pKa = 3.33AVVEE29 pKa = 4.1GDD31 pKa = 3.7TEE33 pKa = 4.82GEE35 pKa = 4.27HH36 pKa = 6.37CQDD39 pKa = 3.53AAMEE43 pKa = 4.39GDD45 pKa = 4.9AEE47 pKa = 4.95GEE49 pKa = 4.11QCDD52 pKa = 4.5AISPAKK58 pKa = 9.13TQDD61 pKa = 3.17TASQDD66 pKa = 3.33AVVEE70 pKa = 4.1GDD72 pKa = 3.7TEE74 pKa = 4.82GEE76 pKa = 4.27HH77 pKa = 6.37CQDD80 pKa = 3.53AAMEE84 pKa = 4.39GDD86 pKa = 4.9AEE88 pKa = 4.95GEE90 pKa = 4.11QCDD93 pKa = 4.5AISPAKK99 pKa = 9.13TQDD102 pKa = 3.17TASQDD107 pKa = 3.33AVVEE111 pKa = 4.1GDD113 pKa = 3.7TEE115 pKa = 4.82GEE117 pKa = 4.27HH118 pKa = 6.37CQDD121 pKa = 3.53AAMEE125 pKa = 4.39GDD127 pKa = 4.9AEE129 pKa = 4.95GEE131 pKa = 4.06QCDD134 pKa = 4.55AISLAKK140 pKa = 9.26TQDD143 pKa = 3.08TARR146 pKa = 11.84QDD148 pKa = 3.44AVVEE152 pKa = 4.09GDD154 pKa = 3.7TEE156 pKa = 4.96GEE158 pKa = 4.24HH159 pKa = 6.27CQNAAMEE166 pKa = 4.51GDD168 pKa = 4.26AEE170 pKa = 4.95GEE172 pKa = 4.11QCDD175 pKa = 4.5AISPAKK181 pKa = 9.13TQDD184 pKa = 3.17TASQDD189 pKa = 3.33AVVEE193 pKa = 4.1GDD195 pKa = 3.7TEE197 pKa = 4.82GEE199 pKa = 4.27HH200 pKa = 6.37CQDD203 pKa = 3.53AAMEE207 pKa = 4.39GDD209 pKa = 3.64AEE211 pKa = 4.74GEE213 pKa = 4.13QFTCLPCQNTSSQILFSFFRR233 pKa = 11.84VGGSAQRR240 pKa = 11.84MPFTKK245 pKa = 10.33HH246 pKa = 4.94

Molecular weight: 25.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3J4D4|A0A2V3J4D4_9FLOR Cytochrome c oxidase assembly protein COX20 mitochondrial OS=Gracilariopsis chorda OX=448386 GN=BWQ96_01028 PE=3 SV=1
MM1 pKa = 6.93STVAQSWSSPLCMEE15 pKa = 4.35TWHH18 pKa = 6.82RR19 pKa = 11.84ALSNAEE25 pKa = 3.91GRR27 pKa = 11.84DD28 pKa = 3.28KK29 pKa = 11.09LLRR32 pKa = 11.84LAQYY36 pKa = 10.03SCKK39 pKa = 10.01LLRR42 pKa = 11.84GIEE45 pKa = 4.02QNKK48 pKa = 9.09PFTSSSSRR56 pKa = 11.84TLLALEE62 pKa = 4.7SALSTSRR69 pKa = 11.84QIGRR73 pKa = 11.84LLKK76 pKa = 8.67WTSIYY81 pKa = 10.92AKK83 pKa = 9.95RR84 pKa = 11.84RR85 pKa = 11.84STSSPSAASSSQQTLNVLSDD105 pKa = 3.49VALFGYY111 pKa = 7.1YY112 pKa = 10.45LCDD115 pKa = 3.8NLTFLCKK122 pKa = 10.01TGVIGGDD129 pKa = 3.09VGRR132 pKa = 11.84ASRR135 pKa = 11.84RR136 pKa = 11.84AARR139 pKa = 11.84FWMASILTGMIGTLFSLLQIRR160 pKa = 11.84RR161 pKa = 11.84QAILLRR167 pKa = 11.84RR168 pKa = 11.84TLSQSDD174 pKa = 4.14DD175 pKa = 3.81KK176 pKa = 11.36QSSASRR182 pKa = 11.84VCEE185 pKa = 3.97RR186 pKa = 11.84NLRR189 pKa = 11.84TYY191 pKa = 10.9GALQRR196 pKa = 11.84TRR198 pKa = 11.84CVTLMKK204 pKa = 10.31QVADD208 pKa = 4.29VIISMSLSQEE218 pKa = 4.0KK219 pKa = 10.06PMHH222 pKa = 5.66NAITGTCGVVSSTIACSQIWPTSPSTRR249 pKa = 3.42

Molecular weight: 27.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10593

0

10593

4111427

24

5424

388.1

43.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.712 ± 0.024

1.612 ± 0.013

5.532 ± 0.017

6.209 ± 0.028

3.731 ± 0.013

6.012 ± 0.027

2.665 ± 0.014

4.572 ± 0.014

4.857 ± 0.022

9.108 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.01

3.758 ± 0.012

5.438 ± 0.025

3.86 ± 0.017

7.067 ± 0.019

8.454 ± 0.027

5.593 ± 0.016

6.98 ± 0.02

1.092 ± 0.008

2.464 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski