Ruminiclostridium herbifermentans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminiclostridium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3957 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U7JDP9|A0A4U7JDP9_9FIRM Primosomal protein N' OS=Ruminiclostridium herbifermentans OX=2488810 GN=priA PE=3 SV=1
MM1 pKa = 6.93ATEE4 pKa = 5.24LIVNGGFEE12 pKa = 4.44TGSFPPWGAVEE23 pKa = 3.99ATITSLFSHH32 pKa = 6.97TGTYY36 pKa = 9.87SAQLQNGTSVIYY48 pKa = 7.84QTVYY52 pKa = 11.17GDD54 pKa = 3.6FSQPVEE60 pKa = 4.14VSAYY64 pKa = 8.33LAKK67 pKa = 10.71VGASPNPIVSIVLSYY82 pKa = 10.99FDD84 pKa = 4.26NSFNFLGTGLVISIPEE100 pKa = 4.02SNIPDD105 pKa = 3.86VIGGDD110 pKa = 3.16WLKK113 pKa = 11.09AVGTSIPAPVGTDD126 pKa = 3.48FAILSISKK134 pKa = 8.39NTLAGGADD142 pKa = 3.6VLVDD146 pKa = 3.86DD147 pKa = 4.97VSILEE152 pKa = 4.67GPVSPTGPTGPSGPSGPTGPTGPTGPTGPTGPTGPTGPTGPGGGATGPTGPTGPTGPAGATGPTGPAGITGPTGPTGPAGATGPTGPVGPTGPGGGATGPTGPAGATGPTGPTGPAGVTGPTGPVGPTGPGGGATGPTGPAGATGPTGPAGTTGPTGPAGVTGPTGPGGGATGPTGPAGATGPTGPAGATGPTGPAGVTGPTGPIGPTGPGGGATGPTGPAGATGPTGPAGVTGPTGITGTTGPAGATGPTGPAGATGPTGPAGATGPTGPAGTTGPTGPAGVTGPTGADD442 pKa = 3.47GATGPTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPIGPTGPAGSTGPAGVTGATGPAGVTGPTGITGPTGPAGATGPTGPAGATGPTGPAGITGPTGPAGTTGPTGPAGTTGPAGLEE628 pKa = 4.08AFGGLYY634 pKa = 9.25STDD637 pKa = 3.49SQTLSIGIGGGVDD650 pKa = 3.43QIEE653 pKa = 4.51LAEE656 pKa = 4.38TMPSSDD662 pKa = 3.13VTLGTNNITILDD674 pKa = 3.8SGTYY678 pKa = 9.04EE679 pKa = 3.64IEE681 pKa = 4.15YY682 pKa = 8.85MVTVGTVVLAVNLSAGVRR700 pKa = 11.84VNGGTNFIPSTFQTRR715 pKa = 11.84LLSLTLTTLFEE726 pKa = 4.65GSTIVDD732 pKa = 4.36LAAGDD737 pKa = 4.31VIDD740 pKa = 5.71LAVQSTDD747 pKa = 3.16SVSASLTGGLTAYY760 pKa = 8.69LTVKK764 pKa = 10.26KK765 pKa = 10.63ISII768 pKa = 3.97

Molecular weight: 68.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U7JIE3|A0A4U7JIE3_9FIRM Iron ABC transporter permease OS=Ruminiclostridium herbifermentans OX=2488810 GN=EHE19_000535 PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.82QPKK8 pKa = 9.61KK9 pKa = 8.53RR10 pKa = 11.84HH11 pKa = 5.03RR12 pKa = 11.84QKK14 pKa = 10.33EE15 pKa = 3.73HH16 pKa = 5.99GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 7.55VLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 9.75GRR39 pKa = 11.84KK40 pKa = 9.04VISAA44 pKa = 4.05

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3957

0

3957

1336279

14

5773

337.7

37.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.321 ± 0.046

1.285 ± 0.016

5.629 ± 0.039

6.827 ± 0.045

4.279 ± 0.041

6.521 ± 0.111

1.384 ± 0.016

9.203 ± 0.058

7.957 ± 0.049

8.75 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.022

6.136 ± 0.043

3.147 ± 0.052

2.839 ± 0.021

3.508 ± 0.031

6.714 ± 0.042

5.545 ± 0.116

6.398 ± 0.035

0.852 ± 0.015

4.221 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski