Streptomyces sp. HGB0020

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S2YFN9|S2YFN9_9ACTN IMP dehydrogenase OS=Streptomyces sp. HGB0020 OX=1078086 GN=HMPREF1211_04756 PE=3 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84SRR4 pKa = 11.84PARR7 pKa = 11.84HH8 pKa = 5.88RR9 pKa = 11.84LIGAVPAVTLALGAALAVPLTTAGSAAAATTPATASVNEE48 pKa = 4.16YY49 pKa = 10.49DD50 pKa = 3.11WQLTYY55 pKa = 10.68RR56 pKa = 11.84AAPGQANTAMVTASYY71 pKa = 8.9TADD74 pKa = 3.4RR75 pKa = 11.84TGFTYY80 pKa = 10.8VIDD83 pKa = 4.08DD84 pKa = 4.21VVPIDD89 pKa = 4.23AGHH92 pKa = 6.58GCTYY96 pKa = 10.96LDD98 pKa = 3.23TSDD101 pKa = 3.61RR102 pKa = 11.84TKK104 pKa = 10.85VSCTVADD111 pKa = 4.15VEE113 pKa = 4.85SQDD116 pKa = 3.92PYY118 pKa = 10.5PALVMDD124 pKa = 5.73LGDD127 pKa = 4.73GDD129 pKa = 5.89DD130 pKa = 3.53KK131 pKa = 11.55AAYY134 pKa = 10.13NNATGQVYY142 pKa = 10.05SYY144 pKa = 11.97AEE146 pKa = 3.84ISLGAGDD153 pKa = 4.91DD154 pKa = 3.82HH155 pKa = 8.96AIDD158 pKa = 3.58TGRR161 pKa = 11.84LDD163 pKa = 3.28GAYY166 pKa = 10.46VSGDD170 pKa = 3.51AGDD173 pKa = 3.87DD174 pKa = 4.07TITVGADD181 pKa = 3.13GFAWGGDD188 pKa = 3.71DD189 pKa = 5.23NDD191 pKa = 4.62TIFANGGDD199 pKa = 4.01NIVKK203 pKa = 10.44GGAGDD208 pKa = 4.49DD209 pKa = 3.96EE210 pKa = 4.82LHH212 pKa = 6.76GGDD215 pKa = 4.3GAQDD219 pKa = 3.7LSGDD223 pKa = 4.09DD224 pKa = 4.98GNDD227 pKa = 3.15TLYY230 pKa = 11.24GGTGDD235 pKa = 4.01DD236 pKa = 3.57SLYY239 pKa = 10.85GGKK242 pKa = 10.66GNDD245 pKa = 3.21VIYY248 pKa = 10.71GNSGNDD254 pKa = 3.36TLYY257 pKa = 11.29GNSGNDD263 pKa = 3.38TLYY266 pKa = 10.99GGPGTDD272 pKa = 3.65TLSGGPGTNVIHH284 pKa = 6.93QDD286 pKa = 2.99

Molecular weight: 28.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S2XUW4|S2XUW4_9ACTN NADH-quinone oxidoreductase subunit H OS=Streptomyces sp. HGB0020 OX=1078086 GN=nuoH PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8369

0

8369

2744775

20

2763

328.0

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.258 ± 0.034

0.784 ± 0.007

6.048 ± 0.024

5.641 ± 0.028

2.78 ± 0.014

9.318 ± 0.029

2.369 ± 0.014

3.242 ± 0.017

2.274 ± 0.022

10.283 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.011

1.841 ± 0.016

5.986 ± 0.026

2.893 ± 0.016

7.894 ± 0.035

5.201 ± 0.022

6.229 ± 0.023

8.49 ± 0.024

1.543 ± 0.01

2.166 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski