Firmicutes bacterium CAG:129

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1635 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5RXY5|R5RXY5_9FIRM CRM domain-containing protein OS=Firmicutes bacterium CAG:129 OX=1263003 GN=BN483_01176 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.51KK3 pKa = 10.24FFALLLSLAMVLALVACGQKK23 pKa = 10.67DD24 pKa = 3.99PTPDD28 pKa = 3.51TPDD31 pKa = 3.68TPDD34 pKa = 3.78DD35 pKa = 4.07PQTKK39 pKa = 9.54VEE41 pKa = 4.48YY42 pKa = 9.08KK43 pKa = 10.02VAMITDD49 pKa = 3.77YY50 pKa = 11.88GDD52 pKa = 3.15ITDD55 pKa = 3.56QSFNQTTYY63 pKa = 9.78EE64 pKa = 3.85ACKK67 pKa = 10.57AFAEE71 pKa = 4.56DD72 pKa = 3.5NSIEE76 pKa = 3.92FSYY79 pKa = 10.24FKK81 pKa = 10.48PAGDD85 pKa = 3.56NTADD89 pKa = 3.34RR90 pKa = 11.84VAMIEE95 pKa = 4.04KK96 pKa = 10.43AVDD99 pKa = 2.89EE100 pKa = 4.58GFNVIVMPGYY110 pKa = 10.78AFGGAIVEE118 pKa = 4.4AAPEE122 pKa = 4.04FPDD125 pKa = 3.52VKK127 pKa = 10.82FIALDD132 pKa = 3.55VAAGDD137 pKa = 4.03LLEE140 pKa = 4.33TAVAKK145 pKa = 10.76AGEE148 pKa = 4.49TYY150 pKa = 10.49DD151 pKa = 3.78YY152 pKa = 11.19TPEE155 pKa = 3.65NWDD158 pKa = 3.39LNKK161 pKa = 10.52YY162 pKa = 10.3VDD164 pKa = 3.84MSNVYY169 pKa = 9.5CAVYY173 pKa = 10.27QEE175 pKa = 4.43EE176 pKa = 4.89LCGYY180 pKa = 7.1MAGYY184 pKa = 10.11AAVKK188 pKa = 10.3LGYY191 pKa = 10.22KK192 pKa = 10.24NLGFLGGMSVPAVVRR207 pKa = 11.84YY208 pKa = 9.95GYY210 pKa = 11.28GFVQGVDD217 pKa = 3.08AAAADD222 pKa = 4.35LGLTDD227 pKa = 3.7VKK229 pKa = 11.23VNYY232 pKa = 9.77IYY234 pKa = 10.94GGQFFGDD241 pKa = 3.47ADD243 pKa = 3.44ITAVMDD249 pKa = 3.26TWYY252 pKa = 10.71QGGTEE257 pKa = 4.26VVFACGGGIYY267 pKa = 10.05TSAVDD272 pKa = 3.29AAKK275 pKa = 10.44KK276 pKa = 10.37VGAKK280 pKa = 10.24VIGVDD285 pKa = 3.23VDD287 pKa = 3.55QAGVIAKK294 pKa = 9.65YY295 pKa = 10.54AGVDD299 pKa = 3.38GMTVTSAMKK308 pKa = 10.1GLYY311 pKa = 8.55PATYY315 pKa = 7.84DD316 pKa = 3.29TLTDD320 pKa = 3.88VIVNGNWDD328 pKa = 3.63KK329 pKa = 11.49YY330 pKa = 8.69VGKK333 pKa = 10.12IATLGLVSGTDD344 pKa = 3.29PEE346 pKa = 4.38ANYY349 pKa = 10.28VQIPMGDD356 pKa = 3.39GTQWSDD362 pKa = 3.87SFTQDD367 pKa = 3.53DD368 pKa = 4.39YY369 pKa = 12.11KK370 pKa = 11.99AMVKK374 pKa = 10.73DD375 pKa = 3.64MFDD378 pKa = 3.36GKK380 pKa = 9.43ITVSNNTSSDD390 pKa = 3.01VSAADD395 pKa = 3.47FATVITVDD403 pKa = 3.66DD404 pKa = 3.67QGSIKK409 pKa = 10.7GG410 pKa = 3.59

Molecular weight: 43.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5RW71|R5RW71_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:129 OX=1263003 GN=BN483_00686 PE=4 SV=1
MM1 pKa = 7.31AAAVPALVLFAATTLLAVFFLAASYY26 pKa = 7.64PTLCAAAKK34 pKa = 10.05RR35 pKa = 11.84RR36 pKa = 11.84LSPRR40 pKa = 11.84AQQRR44 pKa = 11.84LRR46 pKa = 11.84FWRR49 pKa = 11.84DD50 pKa = 2.7GVTHH54 pKa = 7.06SLTRR58 pKa = 11.84WLRR61 pKa = 11.84AQAILCSLTFCQLLAGFWLLGEE83 pKa = 4.71RR84 pKa = 11.84YY85 pKa = 9.62SLLAAFLITLVDD97 pKa = 3.96ALPVFGTGTVLIPWALAEE115 pKa = 4.42LLLGSVPRR123 pKa = 11.84GAALVILYY131 pKa = 9.88LCTLTVRR138 pKa = 11.84SITEE142 pKa = 3.93PKK144 pKa = 10.19LVAAQAGLPPIASLMAMYY162 pKa = 10.53LGARR166 pKa = 11.84AFGVAGMVLFPLLLLLAAQMLRR188 pKa = 11.84TKK190 pKa = 10.71KK191 pKa = 9.99EE192 pKa = 3.57GSMM195 pKa = 3.99

Molecular weight: 20.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1635

0

1635

484243

29

1862

296.2

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.435 ± 0.08

1.697 ± 0.031

5.622 ± 0.053

6.968 ± 0.059

3.733 ± 0.046

7.958 ± 0.051

1.909 ± 0.037

5.372 ± 0.058

4.683 ± 0.061

10.174 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.03

3.094 ± 0.031

3.913 ± 0.039

2.878 ± 0.033

6.043 ± 0.061

5.272 ± 0.048

5.642 ± 0.061

7.415 ± 0.051

0.937 ± 0.024

3.436 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski