Rhodococcus sp. B7740

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D5AAE5|A0A0D5AAE5_9NOCA Phosphomannomutase OS=Rhodococcus sp. B7740 OX=1564114 GN=NY08_1756 PE=3 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84DD3 pKa = 3.51QLPPGLPPDD12 pKa = 4.62PFAGDD17 pKa = 3.58PADD20 pKa = 4.15PSAALDD26 pKa = 4.6AIEE29 pKa = 5.17PGQPLDD35 pKa = 3.61PQEE38 pKa = 4.14RR39 pKa = 11.84LAVEE43 pKa = 4.17EE44 pKa = 4.75DD45 pKa = 3.54LADD48 pKa = 3.98LAVYY52 pKa = 8.37EE53 pKa = 4.5ALLGHH58 pKa = 6.61RR59 pKa = 11.84GIRR62 pKa = 11.84GLVVCCEE69 pKa = 4.59DD70 pKa = 3.72CQQDD74 pKa = 4.28HH75 pKa = 5.29YY76 pKa = 11.18HH77 pKa = 6.72DD78 pKa = 4.0WDD80 pKa = 3.57MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.32EE99 pKa = 4.33PAYY102 pKa = 10.81DD103 pKa = 4.06PVPDD107 pKa = 5.48AYY109 pKa = 10.2VTWDD113 pKa = 3.46YY114 pKa = 11.76CRR116 pKa = 11.84GFADD120 pKa = 4.63ASMNDD125 pKa = 3.52ALHH128 pKa = 7.08GDD130 pKa = 4.07GFDD133 pKa = 3.49SS134 pKa = 4.33

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D5AIY5|A0A0D5AIY5_9NOCA Uncharacterized protein OS=Rhodococcus sp. B7740 OX=1564114 GN=NY08_5073 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84NAKK5 pKa = 8.67QWIIAALAAVAGFALMNFGNIASSIVILVLAVGFVLALTTPVLYY49 pKa = 9.84PRR51 pKa = 11.84SLSDD55 pKa = 3.48DD56 pKa = 3.42ASRR59 pKa = 11.84TLATRR64 pKa = 11.84NSVPLVYY71 pKa = 9.71WRR73 pKa = 11.84PGCIFCLRR81 pKa = 11.84LRR83 pKa = 11.84VALLLRR89 pKa = 11.84GKK91 pKa = 10.09KK92 pKa = 9.92AVWVNIRR99 pKa = 11.84KK100 pKa = 9.7DD101 pKa = 3.44PAGVEE106 pKa = 3.78PAEE109 pKa = 4.3RR110 pKa = 11.84PFNAAAARR118 pKa = 11.84VRR120 pKa = 11.84SVNDD124 pKa = 3.4GNEE127 pKa = 4.0TVPTVFVGSEE137 pKa = 3.72HH138 pKa = 6.3RR139 pKa = 11.84TNPDD143 pKa = 3.39PSWVLAQFVV152 pKa = 3.42

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5193

0

5193

1625198

37

8921

313.0

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.486 ± 0.039

0.709 ± 0.009

6.48 ± 0.03

5.412 ± 0.028

3.127 ± 0.021

8.783 ± 0.035

2.078 ± 0.016

4.482 ± 0.022

2.211 ± 0.023

9.826 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.013

2.162 ± 0.015

5.313 ± 0.025

2.691 ± 0.018

6.966 ± 0.034

6.35 ± 0.025

6.398 ± 0.025

9.109 ± 0.034

1.396 ± 0.014

2.024 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski