Flavobacterium sp. HMWF030

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7JQY6|A0A2R7JQY6_9FLAO Cytochrome C oxidase Cbb3 (Fragment) OS=Flavobacterium sp. HMWF030 OX=2056864 GN=DBR27_24105 PE=4 SV=1
WW1 pKa = 5.48TTGTVNNGEE10 pKa = 4.25TQTLDD15 pKa = 2.54IYY17 pKa = 11.59AKK19 pKa = 10.6VNTPSGTANEE29 pKa = 4.33YY30 pKa = 9.93TNEE33 pKa = 4.09TEE35 pKa = 4.24IVASNMPDD43 pKa = 3.52PDD45 pKa = 3.7STPNNGIKK53 pKa = 9.56TEE55 pKa = 4.31DD56 pKa = 4.51DD57 pKa = 3.49YY58 pKa = 12.2DD59 pKa = 4.62SIQINVPIADD69 pKa = 4.22LSLDD73 pKa = 3.43KK74 pKa = 10.79TVSNKK79 pKa = 9.72NPNIDD84 pKa = 3.55EE85 pKa = 4.79VITFTIQLNNAGPSQATGVAIEE107 pKa = 4.94DD108 pKa = 4.62IIPLGYY114 pKa = 10.54RR115 pKa = 11.84NITNITNGGLFSKK128 pKa = 10.83NAIKK132 pKa = 9.68WVNLTVPVGGITLTYY147 pKa = 9.66QVTVVSPHH155 pKa = 6.22GLDD158 pKa = 3.55GYY160 pKa = 11.13DD161 pKa = 3.74YY162 pKa = 11.62LNIAQVTASDD172 pKa = 4.32QYY174 pKa = 12.08DD175 pKa = 3.9PDD177 pKa = 4.12SKK179 pKa = 11.15PNNDD183 pKa = 4.34NGDD186 pKa = 3.43QSEE189 pKa = 4.41DD190 pKa = 3.91DD191 pKa = 4.11EE192 pKa = 5.91DD193 pKa = 4.63SEE195 pKa = 5.61FINISATDD203 pKa = 3.12IGINKK208 pKa = 9.4EE209 pKa = 3.46VDD211 pKa = 3.6QINVPMYY218 pKa = 9.41STVTFTITAEE228 pKa = 4.02NLGNLTATNVEE239 pKa = 4.31VLDD242 pKa = 4.44ILPKK246 pKa = 10.5GYY248 pKa = 10.17QLDD251 pKa = 4.11TFTVSAGVYY260 pKa = 10.41NSTTGIWTIPTIASKK275 pKa = 10.86GSQTLAISAKK285 pKa = 9.9VVDD288 pKa = 4.93FNDD291 pKa = 3.73YY292 pKa = 11.26LNTAQFVKK300 pKa = 9.94MDD302 pKa = 4.11QIDD305 pKa = 4.17SNSSNNQDD313 pKa = 3.38SATVAPNCLKK323 pKa = 10.36IFNEE327 pKa = 4.76FSPNDD332 pKa = 3.76DD333 pKa = 3.65GQNDD337 pKa = 3.99TFYY340 pKa = 10.59IDD342 pKa = 5.71CISQYY347 pKa = 10.69PDD349 pKa = 2.91NQLQIFNRR357 pKa = 11.84WGNLVYY363 pKa = 10.65YY364 pKa = 10.42KK365 pKa = 10.36KK366 pKa = 10.72GYY368 pKa = 9.65NNTWDD373 pKa = 3.54GKK375 pKa = 10.9ADD377 pKa = 3.75GSAKK381 pKa = 9.56TLPEE385 pKa = 3.5GTYY388 pKa = 10.11FYY390 pKa = 10.74ILDD393 pKa = 4.72LGNGSPKK400 pKa = 9.9TSGWLYY406 pKa = 11.17LKK408 pKa = 10.73

Molecular weight: 44.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7L974|A0A2R7L974_9FLAO Peptide chain release factor 3 (Fragment) OS=Flavobacterium sp. HMWF030 OX=2056864 GN=prfC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4740

0

4740

1334036

16

2144

281.4

31.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.518 ± 0.038

0.761 ± 0.012

5.37 ± 0.027

6.209 ± 0.04

5.361 ± 0.029

6.232 ± 0.036

1.632 ± 0.015

8.154 ± 0.036

8.197 ± 0.041

9.181 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.019

6.425 ± 0.04

3.414 ± 0.02

3.419 ± 0.021

3.133 ± 0.021

6.629 ± 0.031

5.938 ± 0.043

6.13 ± 0.028

1.077 ± 0.013

4.115 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski